<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ali Mohammed Mohammed Ali Al-Samman</style></author><author><style face="normal" font="default" size="100%">Kahkashan</style></author><author><style face="normal" font="default" size="100%">Nadeem Ahmad Siddique</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Gas Chromatography–Mass Spectrometry (GC-MS/MS) Analysis, Ultrasonic Assisted Extraction, Antibacterial and Antifungal Activity of Emblica officinalis Fruit Extract</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antibacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Antifungal</style></keyword><keyword><style  face="normal" font="default" size="100%">Emblica officinalis</style></keyword><keyword><style  face="normal" font="default" size="100%">GC-MS/MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Ultrasonic assisted extraction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">315-323</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;!-- x-tinymce/html --&gt;&lt;strong&gt;Introduction&lt;/strong&gt;: &lt;em&gt;Emblica officinalis&lt;/em&gt; is one of the common plants being used traditionally in different ways to search for cures and relief from various diseases. Among these diseases,&lt;em&gt; Emblica officinalis&lt;/em&gt; had many of medicinal uses in treating a wide variety of bacterial and fungal infections. In this study, Ultrasonic assisted extraction (UAE) was efficiently used for the preparation of aqueous and methanolic extracts of &lt;em&gt;Embelica officinalis&lt;/em&gt; fruits (EOFE). The phytoconstituents was detected through GC-MS/MS analysis to confirm antimicrobial effect of EOFE. &lt;strong&gt;Materials and methods&lt;/strong&gt;: The extraction was carried out at 20ºC, for 20 min using a solid-to-solvent ratio of 1: 60 w/v. The extracts were subjected to GC-MS/MS analysis. The &lt;em&gt;in-vitro&lt;/em&gt; effect of extracts against twenty one microbial strains was investigated by an agar well diffusion method in different concentrations (25 μg/mL-1000 μg/mL). &lt;strong&gt;Results&lt;/strong&gt;: The percentage yield was found to be 41.33% w/v and 23.0 % w/v with water and methanol, respectively. Phenol,3,5-bis1,1 dimethylethyl, 2,4-di-tert-butylphenol and heptasiloxa ne1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethyl were confirmed by GC-MS/MS analysis. Effectiveness of extracts against Gram negative bacteria; &lt;em&gt;Providencia alcalifaciens&lt;/em&gt; and Gram positive bacteria; &lt;em&gt;Bacillus pumilis, Bacillus polymyxa and fungal strains; Neurospora crassa, Aspergillus brasileinsis and Cladosporium oxysporum&lt;/em&gt; are reported for the first time. Aqueous extract revealed excellent antibacterial activity at 50 μg/mL and antifungal activity at 100 μg/mL whereas methanolic extract showed antifungal activity at 250 μg/mL. &lt;strong&gt;Conclusion&lt;/strong&gt;: Results highlight the considerable inhibitory effect of EOFE against various microbial species was mainly due to the presence of phenolic compounds and other phytocompounds.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">315</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;!-- x-tinymce/html --&gt;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Ali Mohammed Mohammed Ali Al-Samman&lt;sup&gt;1&lt;/sup&gt;, Kahkashan&lt;sup&gt;2&lt;/sup&gt;, Nadeem Ahmad Siddique&lt;sup&gt;1,&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Bioactive Natural Compound Laboratory (BNCL); Department of Pharmacognosy and Phytochemistry, Glocal School of Pharmacy, Glocal University, Saharanpur- 247121, Uttar Pradesh, INDIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Computer Science and Bio Informatics, Faculty of Natural Science. Jamia Millia Islamia, New Delhi, Delhi- 110025, INDIA.&lt;/p&gt;
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