<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Thanyathorn Tangsongcharoen</style></author><author><style face="normal" font="default" size="100%">Somchai Issaravanich</style></author><author><style face="normal" font="default" size="100%">Chanida Palanuvej</style></author><author><style face="normal" font="default" size="100%">Nijsiri Ruangrungsi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Quantitative Analysis of Hispidulin Content in Clerodendrum petasites Roots Distributed in Thailand</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ben-Cha-Lo-Ka-Wi-Chian remedy</style></keyword><keyword><style  face="normal" font="default" size="100%">Clerodendrum petasites</style></keyword><keyword><style  face="normal" font="default" size="100%">hispidulin</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC-PDA</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">1093-1099</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Clerodendrum petasites&lt;/em&gt; (Lour.) S. Moore (locally known as Mai-Thao-Yaai-Mom), belonging to the Verbenaceae family, is widely formulated into multi-herb remedy, Ben-Cha- Lo-Ka-Wi-Chian remedy, possessing antipyretic activity. &lt;em&gt;C. petasites&lt;/em&gt; exhibits many biological activities, such as antioxidant, anti-inflammatory, antipyretic, etc. The flavonoid hispidulin is one of the main active compounds present in &lt;em&gt;C. petasites&lt;/em&gt;, containing anti-atheromatous, antitumor and antispasmodic effects. &lt;strong&gt;Objective:&lt;/strong&gt; The present study aimed to determine the hispidulin content in the dried roots of &lt;em&gt;C. petasites &lt;/em&gt;using HPLC technique.&lt;strong&gt; Methods:&lt;/strong&gt; &lt;em&gt;C. petasites &lt;/em&gt;dried roots, collected from twelve different areas, were extracted with ethanol using Soxhlet apparatus, and then subjected to HPLC-PDA to quantify hispidulin content. The quantitative method using HPLC-PDA technique was validated.&lt;strong&gt; Results:&lt;/strong&gt; The optimized HPLC coupling with PDA detector (HPLC-PDA) was validated for the quantitative analysis of hispidulin content in &lt;em&gt;C. petasites&lt;/em&gt; roots in terms of linearity (y = 210,200,536.6667x – 448,756.2667; R&lt;sup&gt;2&lt;/sup&gt; = 0.9997), accuracy (88.82-107.69% recovery), precision (0.66% RSD for repeatability precision; 1.17% RSD for intermediate precision), limit of detection (2.30 μg/mL), limit of quantitation (7.00 μg/mL), specificity (peak purity index = 1.0000) and robustness (% RSD &amp;lt; 1). The amount of hispidulin content in the extracts of &lt;em&gt;C. petasites&lt;/em&gt; roots conducted from the validated method was found to be 0.0182 ± 0.0109 g/100 g crude drug. &lt;strong&gt;Conclusion: &lt;/strong&gt;The HPLC-PDA analysis was able to effectively determine hispidulin in &lt;em&gt;C. petasites&lt;/em&gt; roots. The hispidulin contents in &lt;em&gt;C. petasites&lt;/em&gt; dried roots from various areas in Thailand were revealed which could be used for the specification of this crude drug with reference to its chemical marker.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1093</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Thanyathorn Tangsongcharoen&lt;sup&gt;1&lt;/sup&gt;, Somchai Issaravanich&lt;sup&gt;1&lt;/sup&gt;, Chanida Palanuvej&lt;sup&gt;1,&lt;/sup&gt;* , Nijsiri Ruangrungsi&lt;sup&gt;1,2&lt;/sup&gt;&lt;/strong&gt;&lt;sup&gt; &lt;/sup&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Public Health Sciences Program, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, THAILAND.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacognosy, College of Pharmacy, Rangsit University, Pathum Thani 12000, THAILAND.&lt;/p&gt;
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