<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Boualem HARFI</style></author><author><style face="normal" font="default" size="100%">Lakhdar KHELIFI</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Hairy Root Technology: Effect of Etiolation on Datura sp. Transgenic Root Induction and Hyoscyamine Production</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Datura sp.</style></keyword><keyword><style  face="normal" font="default" size="100%">Etiolation</style></keyword><keyword><style  face="normal" font="default" size="100%">Gas chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">Hairy roots</style></keyword><keyword><style  face="normal" font="default" size="100%">Hyoscyamine</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">991-995</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Hyoscyamine, because of its medicinal properties, is an important tropane alkaloid. In order to implement a biotechnological process for its production, hairy roots of&lt;em&gt; Datura &lt;/em&gt;species resulting from genetic transformation by &lt;em&gt;Agrobacterium rhizogenes&lt;/em&gt; A4 strain have been the subject of this work. In the &lt;em&gt;in vitro &lt;/em&gt;alkaloids production programs, optimization of the transformation is a crucial step for obtaining a large number of root lines allowing the selection of efficient lines. &lt;strong&gt;Methods:&lt;/strong&gt; The conditions for hairy roots induction were explored by studying the effect of &lt;em&gt;Datura&lt;/em&gt; sp. &lt;em&gt;in vitro&lt;/em&gt; seedling etiolation on the genetic transformation. The first step was the establishment of &lt;em&gt;Datura&lt;/em&gt; &lt;em&gt;in vitro&lt;/em&gt; plantlet cultures followed by the hairy roots induction with A4 strain of &lt;em&gt;A. rhizogenes&lt;/em&gt;. The confirmation of the genetic transformation was performed by polymerase chain reaction (PCR) analysis of rolB gene in the roots. After hyoscyamine extraction, it was analyzed (quantitatively and qualitatively) by gas chromatography. Results: 343 root lines were obtained in total, 96 root lines from non-etiolated &lt;em&gt;Datura in vitro&lt;/em&gt; seedlings and 247 root lines from etiolated &lt;em&gt;in vitro&lt;/em&gt; plantlets. After the selection of six transgenic root lines, tow root lines from each species (&lt;em&gt;D. tramonium, D. tatula &lt;/em&gt;and&lt;em&gt; D. innoxia&lt;/em&gt;), one from non-etiolated &lt;em&gt;in vitro &lt;/em&gt;plantlet and one from etiolated &lt;em&gt;in vitro&lt;/em&gt; plantlet, the most hyoscyamine content was 8.43 mg/g D.W. obtained with &lt;em&gt;D. tatula&lt;/em&gt; etiolated &lt;em&gt;in vitro&lt;/em&gt; seedling. &lt;strong&gt;Conclusion: &lt;/strong&gt;The etiolated&lt;em&gt; in vitro&lt;/em&gt; plantlets seem more favorable to hairy roots induction.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">991</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Boualem HARFI&lt;sup&gt;1,2,&lt;/sup&gt;*, Lakhdar KHELIFI&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Biotechnology Research Center (C.R.Bt), Constantine, ALGERIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;National Higher School of Agronomy (ENSA – El-Harrach), Algiers, ALGERIA.&lt;/p&gt;
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