<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Haviah Hafidhotul Ilmiah</style></author><author><style face="normal" font="default" size="100%">Tri Rini Nuringtyas</style></author><author><style face="normal" font="default" size="100%">Laurentius Hartanto Nugroho</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Accumulation of Potential Photo-Protective Compound Groups in Mangrove (Sonneratia caseolaris (L.) Engler.) Leaves</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Leaf anatomy</style></keyword><keyword><style  face="normal" font="default" size="100%">Photo-protective</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolite</style></keyword><keyword><style  face="normal" font="default" size="100%">Sonneratia caseolaris (L.) Engler</style></keyword><keyword><style  face="normal" font="default" size="100%">SPF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/527</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">576-580</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; To analyze the accumulation of potential photo-protective compound groups, include phenol, flavonoid, and tannin in the leaf tissues of &lt;em&gt;Sonneratia caseolaris&lt;/em&gt; (L.) Engler. &lt;strong&gt;Methods:&lt;/strong&gt; The research used &lt;em&gt;Sonneratia caseolaris&lt;/em&gt; (L.) Engler leaves as materials. The total of compound groups and photo-protective ability level of each tissue was measured using spectrophotometry, while detection of compound group accumulations in the leaf tissue were observed using histochemical assay. &lt;strong&gt;Results:&lt;/strong&gt; Epidermis extract contained the highest content of total phenolic compounds (phenol, flavonoid, and tannin), followed by those of mesophyll and whole leaf. Moreover, Sun Protection Factor (SPF) value of epidermis was also higher than those of mesophyll and whole leaf, considering that there were no significant difference of SPF value between mesophyll and whole leaf. Result of histochemical assay of &lt;em&gt;S. caseolaris&lt;/em&gt; fresh leaf sections showed that phenol, flavonoid, and tannin were highly accumulated in the epidermis and some parts of mesophyll. &lt;strong&gt;Conclusion:&lt;/strong&gt; Potential photo-protective compound groups of &lt;em&gt;S. caseolaris&lt;/em&gt; leaves were accumulated in epidermis and mesophyll. However, epidermis showed the highest level of potential photo-protective compound groups than those of other tissues. Photo-protective ability of epidermis also showed higher value than those of mesophyll.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">576</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Haviah Hafidhotul Ilmiah, Tri Rini Nuringtyas, Laurentius Hartanto Nugroho&lt;sup&gt;*&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, 55281, INDONESIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Haviah Hafidhotul Ilmiah</style></author><author><style face="normal" font="default" size="100%">Tri Rini Nuringtyas</style></author><author><style face="normal" font="default" size="100%">Laurentius Hartanto Nugroho</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Accumulation of Potential Photo-Protective Compound Groups in Mangrove (Sonneratia caseolaris (l.) Engler.) Leaves</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Leaf anatomy.</style></keyword><keyword><style  face="normal" font="default" size="100%">Photo-protective</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolite</style></keyword><keyword><style  face="normal" font="default" size="100%">Sonneratia caseolaris (L.) Engler</style></keyword><keyword><style  face="normal" font="default" size="100%">SPF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">x</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">XX-XX</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; To analyze the accumulation of potential photo-protective compound groups, include phenol, flavonoid, and tannin in the leaf tissues of &lt;em&gt;Sonneratia caseolaris&lt;/em&gt; (L.) Engler. &lt;strong&gt;Methods:&lt;/strong&gt; The research used &lt;em&gt;Sonneratia caseolaris&lt;/em&gt; (L.) Engler leaves as materials. The total of compound groups and photo-protective ability level of each tissue was measured using spectrophotometry, while detection of compound group accumulations in the leaf tissue were observed using histochemical assay. &lt;strong&gt;Results:&lt;/strong&gt; Epidermis extract contained the highest content of total phenolic compounds (phenol, flavonoid, and tannin), followed by those of mesophyll and whole leaf. Moreover, Sun Protection Factor (SPF) value of epidermis was also higher than those of mesophyll and whole leaf, considering that there were no significant difference of SPF value between mesophyll and whole leaf. Result of histochemical assay of &lt;em&gt;S. caseolaris&lt;/em&gt; fresh leaf sections showed that phenol, flavonoid, and tannin were highly accumulated in the epidermis and some parts of mesophyll. &lt;strong&gt;Conclusion:&lt;/strong&gt; Potential photo-protective compound groups of &lt;em&gt;S. caseolaris&lt;/em&gt; leaves were accumulated in epidermis and mesophyll. However, epidermis showed the highest level of potential photo-protective compound groups than those of other tissues. Photo-protective ability of epidermis also showed higher value than those of mesophyll.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; &lt;em&gt;Sonneratia caseolaris&lt;/em&gt; (L.) Engler, Secondary metabolite, Photo-protective, SPF, Leaf anatomy.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2s</style></issue><work-type><style face="normal" font="default" size="100%">x</style></work-type><section><style face="normal" font="default" size="100%">x</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Haviah Hafidhotul Ilmiah, Tri Rini Nuringtyas, Laurentius Hartanto Nugroho&lt;sup&gt;* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, 55281, INDONESIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rinto Muhammad Nur</style></author><author><style face="normal" font="default" size="100%">Laurentius Hartanto Nugroho</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic Activities of Fractions from Dioscorea bulbifera L. Chloroform and Methanol Extracts on T47D Breast Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">D. bulbifera</style></keyword><keyword><style  face="normal" font="default" size="100%">MTT Assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword><keyword><style  face="normal" font="default" size="100%">T47D</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/362</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">33-38</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; To elucidate cytotoxic activity of fractions from chloroform and methanol extracts of &lt;em&gt;D. bulbifera &lt;/em&gt;organs on T47D breast cancer cells. &lt;strong&gt;Method:&lt;/strong&gt; The vegetative organs of &lt;em&gt;D. bulbifera&lt;/em&gt; were extracted gradually using chloroform and methanol. Cytotoxicity tested on T47D cells using MTT Assay. The most toxic extract was fractioned by vacuum liquid chromatography (VLC) followed by thin layer chromatography (TLC). The extract and fractions potential were tested on the Vero cells using the same method as cancer cells. The most toxic fraction was analyzed using TLC followed by the application of various spray reagents for the identification of active compound. &lt;strong&gt;Results:&lt;/strong&gt; The chloroform extract of the &lt;em&gt;D. bulbifera &lt;/em&gt;leaves was the highest cytotoxic on T47D cells (IC&lt;sub&gt;50&lt;/sub&gt; 115.63&amp;plusmn;86.01 &amp;mu;g/mL). Moreover, the cytotoxicity test on the combined fractions of leaves chloroform extract showed that fraction 5 (F5) and fraction 6 (F6) were the most toxic fractions compared to those of other fractions. The IC&lt;sub&gt;50&lt;/sub&gt; of both fractions were 14.55&amp;plusmn;8.62 and 7.12&amp;plusmn;4.43 &amp;mu;g/mL respectively. However, Its were very weak compared to those of cancer medicine (Doxorubicin) with the IC&lt;sub&gt;50&lt;/sub&gt; was 0.04&amp;plusmn;0.02 &amp;mu;g/mL. Potential fractions were not toxic against Vero cells with IS&amp;gt;10. The active compounds in those fractions were alkaloid and terpenoid. &lt;strong&gt;Conclusion:&lt;/strong&gt; Chloroform extract of the &lt;em&gt;D. bulbifera&lt;/em&gt; leaves had the highest cytotoxic effect on T47D cells. Potential fractions were not toxic against Vero cells. The active compounds in those fractions were alkaloid and terpenoid.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">33</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Rinto Muhammad Nur&lt;sup&gt;1&lt;/sup&gt;, Laurentius Hartanto Nugroho&lt;sup&gt;2&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Fisheries and Marine Science, Universitas Pasifik Morotai, Jln. Siswa Darame, Kec. Morotae Selatan, Kab. Morotae, Maluku Utara, INDONESIA.&lt;/p&gt;
&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan, Sekip Utara, Yogyakarta, INDONESIA.&lt;/p&gt;</style></auth-address></record></records></xml>