<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Erlena Nor Asmira Abdul Rahim</style></author><author><style face="normal" font="default" size="100%">Muhammad Nor Omar</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antihypertensive Assay-Guided Fractionation of Syzygium polyanthum Leaves and Phenolics Profile Analysis Using LCQTOF/ MS</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antihypertensive</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioassay-guided</style></keyword><keyword><style  face="normal" font="default" size="100%">LCMS</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">Total phenol content (TPC)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1670-1692</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Syzygium polyanthum&lt;/em&gt; leaves extract that contains gallic acid as the major phenolic compound has shown significant antihypertensive effect, however the amount of gallic acid was inversely-related with magnitude of this effect. This study aimed to conduct bioassay-guided fractionation of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves with gallic acid as a reference compound, and to screen for other possible compounds responsible for the antihypertensive effect. &lt;strong&gt;Methods: &lt;/strong&gt;&lt;em&gt;S. polyanthum &lt;/em&gt;leaves were extracted using n-hexane, ethyl acetate, methanol, and water. The most active crude extract was fractionated using column chromatography and analyzed for total phenolic content (TPC) (n=3). Crude extracts and the derived fractions were intravenously administered into pentobarbital-anaesthetized Spontaneously Hypertensive rats (n=5) for recording of blood pressure parameters. Liquid Chromatography-Quadruple Time-Off-Flight/Mass Spectrometry was used for determination of chemical composition. One-way and two-way ANOVA were used for statistical analysis using GraphPad® PRISM Version 6. &lt;strong&gt;Results: &lt;/strong&gt;Fractionation of aqueous &lt;em&gt;S. polyanthum&lt;/em&gt; leaves extract (ASP) afforded nine fractions, later combined into three fractions (F1ASP, F2ASP, and F3ASP) based on the thinlayer chromatography profiles. ASP has the highest TPC while F2ASP has the lowest TPC. All fractions exhibited significant antihypertensive property, but F2ASP was the most active fraction. Few phenolics with related antihypertensive effects such as 1-galloyl glucose (a gallic acid-derivative majorly found in F2ASP and F3ASP), and other compounds such as polydatin, sesamol, brazilin, eugenol, ellagic acid, kukoamine A, and cyclocurcumin were found across all active fractions. &lt;strong&gt;Conclusion: &lt;/strong&gt;These phenolics may partly contribute to the antihypertensive effect of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves, thus further isolation study is recommended.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">167</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Azlini Ismail&lt;sup&gt;1,&lt;/sup&gt;*, Erlena Nor Asmira Abdul Rahim&lt;sup&gt;2&lt;/sup&gt;, Muhammad Nor Omar&lt;sup&gt;2&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kuliyyah of Dentistry, International Islamic University Malaysia, Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biotechnology, Kuliyyah of Science, International Islamic University Malaysia, Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, MALAYSIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Syzygium polyanthum (Wight) Walp: A Potential Phytomedicine</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chemical</style></keyword><keyword><style  face="normal" font="default" size="100%">Nutritional</style></keyword><keyword><style  face="normal" font="default" size="100%">Pharmacological</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">Toxicological</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">429-438</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Ethnomedicinal plant is one of the important sources for drug discovery. &lt;em&gt;Syzygium polyanthum&lt;/em&gt; (Wight) Walp is one of the ethnomedicinal plants that is currently gaining attention for its various pharmacological potentials. This review evaluates its traditional uses, chemical profiles, toxicological aspects, nutritional values, as well as its pharmacological properties. From our literature search in Science Direct, Scopus, and Google Scholar databases, it was found that &lt;em&gt;S. polyanthum&lt;/em&gt; has valuable therapeutic potentials including antidiabetic, antihypertensive, antimicrobial, antioxidant, anticancer, antitumor, antidiarrheal, acetylcholinesterase, inhibitory, and dental plaque inhibition properties. However, few research areas, especially its toxicological profiles, mechanism of actions, as well as identification of its bioactive compound, require more in-depth studies.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Review Article</style></work-type><section><style face="normal" font="default" size="100%">429</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Azlini Ismail&lt;sup&gt;1*&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan, Pahang,&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Nurul Syahida Ramli</style></author><author><style face="normal" font="default" size="100%">Mahaneem Mohamed</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Acute and Sub-Acute Antihypertensive Effects of Syzygium polyanthum Leaf Extracts with Determination of Gallic Acid using HPLC Analysis</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antihypertensive</style></keyword><keyword><style  face="normal" font="default" size="100%">Gallic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Hypertension</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/647</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">663-671</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Context:&lt;/strong&gt; Aqueous decoction of &lt;em&gt;Syzygium polyanthum&lt;/em&gt; (ADSP) leaf is one of Malay traditionalremedial- preparations for hypertension. Intravenous ADSP reduced blood pressure of anesthetized Wistar-Kyoto (WKY) and Spontaneously-Hypertensive rats (SHR); however, acute and sub-acute effects of oral ADSP on conscious rats and its bioactive compound(s) are not comprehensively studied. This study aims to examine the acute and sub-acute effects of ADSP in comparison to methanol extract (MESP) on systolic blood pressure (SBP) of conscious WKY and SHR rats, as well as to identify their major phenolic-compound using highperformance- liquid-chromatography (HPLC). &lt;strong&gt;Methodology:&lt;/strong&gt; For acute study, SHR and WKY rats were gavaged with single-dose of ADSP or MESP (2.00, 2.50 or 3.00 g/kg), vehicles, or losartan (0.01 g/kg). SBP was measured after 1, 3, 5, 6 and 24 hr-post-administration. For subacute experiment, SHR rats were gavaged once-daily with ADSP or MESP (2.50 g/kg/day), vehicles, or losartan (0.01 g/kg/day) for 3-week and their SBP was weekly-measured. Phenolic compounds were screened using ferric-chloride test, then gallic acid was determined using HPLC. &lt;strong&gt;Results:&lt;/strong&gt; Acute administration of ADSP (2.50 to 3.00 g/kg) and MESP (2.00 to 3.00 g/ kg) significantly reduced SBP of SHR, but not of WKY rats. Repeated-daily-dose administration of MESP (2.5 g/kg/day) significantly reduced SBP of SHR after 2-week and sustained until 3-week, while ADSP (2.5 g/kg/day) significantly reduced SBP only after 3-week. Gallic acid was the major phenolic-compound in ADSP (157.09 &amp;plusmn; 4.18 ppm) and MESP (134.04 &amp;plusmn; 10.30 ppm). &lt;strong&gt;Conclusion:&lt;/strong&gt; Oral ADSP and MESP that contain gallic acid possess acute and sub-acute antihypertensive effects in SHR, but MESP&amp;rsquo;s effect was more prominent than ADSP&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">663</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Azlini Ismail&lt;sup&gt;1*&lt;/sup&gt;, Nurul Syahida Ramli&lt;sup&gt;2&lt;/sup&gt;, Mahaneem Mohamed&lt;sup&gt;3&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan Campus, Indera Mahkota, Kuantan, Pahang, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Biomedicine Program, School of Health Sciences, Health Campus, University Sains Malaysia, Kubang Kerian, Kelantan, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Physiology, School of Medical Sciences, Health Campus, University Sains Malaysia, Kubang Kerian, Kelantan, MALAYSIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Erlena Nor Asmira Abd Rahim</style></author><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Muhammad Nor Omar</style></author><author><style face="normal" font="default" size="100%">Umi Nadhirah Rahmat</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">GC-MS Analysis of Phytochemical Compounds in Syzygium polyanthum Leaves Extracted using Ultrasound-Assisted Method</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bioactive compound</style></keyword><keyword><style  face="normal" font="default" size="100%">GC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Sonication</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">Ultra-sound-assisted</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/406</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">110-119</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Context:&lt;/strong&gt; &lt;em&gt;Syzygium polyanthum&lt;/em&gt; has been traditionally formulated by the folklore for the treatment of diseases including diarrhea, rheumatism, diabetes mellitus, hypercholesterolemia, hypertension, gastritis and hyperuricemia. Normally, its phytochemicals are always extracted using solvent, maceration and steam distillation methods, but the use of ultrasound-assisted extraction (UAE) method is still not well documented. &lt;strong&gt;Aims:&lt;/strong&gt; This study aims to extract the phytochemical compounds present in &lt;em&gt;S. polyanthum&lt;/em&gt; leaves using UAE and to identify them by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. &lt;strong&gt;Methodology:&lt;/strong&gt; The leaves were consecutively soaked with n-hexane, ethyl acetate and methanol in a bath sonicator to derive n-hexane (HSP), ethyl acetate (EASP), and methanol (MSP) extracts of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves and then the extracts were subjected to GC-MS analysis. Mass-spectral databases of peaks were compared with database from Wiley, NIST and FNSCC libraries for compound identification. &lt;strong&gt;Results:&lt;/strong&gt; GC-MS analyses of HSP, EASP and MSP showed the presence of 21, 27, and 31 peaks, respectively. The major compound for HSP (31.912%), EASP (27.042%), and MSP (22.386%) were unknown compounds which were detected at retention time between 61.980 and 62.29 min, thus requires further characterization. Squalene and phytol were among the other major compounds present in all three extracts. Several identified compounds in the extracts such as squalene, phytol, hentriacontane, palmitic acid, &amp;alpha;-pinene, nerolidol, linalool, &amp;alpha;-tocopherol and &amp;beta;-tocopherol were known bioactive compounds. &lt;strong&gt;Conclusion:&lt;/strong&gt; GC-MS analyses of n-hexane (HSP), ethyl acetate (EASP), and methanol (MSP) of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves extracts have revealed the presence of some known bioactive compounds with therapeutic importance.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">110</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Erlena Nor Asmira Abd Rahim&lt;sup&gt;1&lt;/sup&gt;, Azlini Ismail&lt;sup&gt;2&lt;/sup&gt;*, Muhammad Nor Omar&lt;sup&gt;1&lt;/sup&gt;, Umi Nadhirah Rahmat&lt;sup&gt;3&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biotechnology, Kuliyyah of Science, Kuantan Campus, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt; 2&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kuliyyah of Dentistry, Kuantan Campus, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;School of Health Sciences, Health Campus, UniversitiSains Malaysia, 16150 KubangKerian, Kelantan, MALAYSIA.&lt;/p&gt;</style></auth-address></record></records></xml>