<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Arfi Rabbani</style></author><author><style face="normal" font="default" size="100%">Anton Bahtiar</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Lince Dameria Nadapdap</style></author><author><style face="normal" font="default" size="100%">Ajeng Megawati Fajrin</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemistry, Antioxidant Activity and Cytotoxicity Evaluation of Black-White Fungus Auricularia sp. against Breast MCF-7 Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">01-07</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Cancer is a non-communicable disease with over 18.1 million new cases and 9.6 million deaths annually according to WHO. Breast cancer is the number two highest count type of cancer trailing behind lung cancer. Treating cancer is expensive and have various side effects. Active components found in plants or fungi that have antioxidant and cytotoxic activity towards cancer cells, could be an alternative for anticancer. One of the fungi that is potentially developed as an anticancer, are the genus of &lt;em&gt;Auricularia sp&lt;/em&gt;. also known as black-white fungus. This study aims to determine the phytochemicals components, antioxidant activity and cytotoxic effect of the &lt;em&gt;Auricularia sp&lt;/em&gt;. towards MCF-7 breast cancer cells. &lt;strong&gt;Methods&lt;/strong&gt;: Dried black-white fungus of&lt;em&gt; Auricularia sp&lt;/em&gt;. grinded into a fine powder. Then, multilevel maceration is done with the n-hexane, ethyl acetate, ethanol as solvents. The extracts of black-white fungus undergo phytochemical screening and thin layer chromatography (TLC), followed by measuring antioxidant and evaluating the cytotoxic activity towards MCF-7 breast cancer cells. &lt;strong&gt;Results&lt;/strong&gt;: black-white fungus of &lt;em&gt;Auricularia sp.&lt;/em&gt; contained secondary metabolites of flavonoids, alkaloids, and triterpenoids and a total of 17 other phytochemical components. Ethyl acetate extract of black-white fungus showed a weak antioxidant activity towards DPPH free radical with IC50 of 215.51 μg/mL and a very active cytotoxic activity on MCF-7 cells with IC&lt;sub&gt;50&lt;/sub&gt; of 0.21 μg/mL. On the other hand, ethanol and n-hexane extracts of black-white fungus are categorized with an active cytotoxic activity on MCF-7 cells with IC&lt;sub&gt;50 &lt;/sub&gt;of 29.28 μg/mL and 50.39 μg/mL, respectively.&lt;strong&gt; Conclusion: &lt;/strong&gt;Black-white fungus &lt;em&gt;Auricularia sp&lt;/em&gt;. that had anticancer activity towards breast MCF-7 cells should be considered as an alternative treatment for breast cancer therapy.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; Phytochemistry, Antioxidant, Cytotoxicity, Black-white fungus Auricularia sp., MCF-7 cells.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">01</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ade Arsianti&lt;sup&gt;1,2,*&lt;/sup&gt;, Arfi Rabbani&lt;sup&gt;3&lt;/sup&gt;, Anton Bahtiar&lt;sup&gt;4&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;2&lt;/sup&gt;, Lince Dameria Nadapdap&lt;sup&gt;2&lt;/sup&gt;, Ajeng Megawati Fajrin&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacology, Faculty of Pharmacy, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nadzila Anindya Tejaputri</style></author><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Fona Qorina</style></author><author><style face="normal" font="default" size="100%">Qotrunnada Fithrotunnisa</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Rista Putrianingsih</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anticancer Activity of Ruellia britoniana Flower on Cervical HeLa Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Cervical HeLa cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Ruellia brittoniana</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">29-34</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Cervical cancer ranks 4&lt;sup&gt;th&lt;/sup&gt; in terms of the mortality rates and incidence of all cancers in women (GLOBOCAN 2018). In last decade, there is a significance progress in cancer therapy followed by an increase in the cost of cancer treatment. Therefore, it is necessary to have therapeutic innovations that are expected to reduce the cost of cervical cancer therapy. One therapeutic innovation that is currently being intensively carried out is herbal medicine. Some researchers have found that some plant extracts have anti-cancer properties that can be an alternative treatment for cancer, such as some plants with the genus &lt;em&gt;Ruellia&lt;/em&gt;, such as &lt;em&gt;Ruellia tuberosa &lt;/em&gt;and &lt;em&gt;Ruellia squarrosa&lt;/em&gt;. However, research on the anticancer activity of the species of &lt;em&gt;Ruellia brittoniana&lt;/em&gt;, especially the flowers, is still limited. &lt;strong&gt;Objective: &lt;/strong&gt;Aim of this study is to examine anti-cervical cancer activity of &lt;em&gt;R. brittoniana&lt;/em&gt; flower. &lt;strong&gt;Methods: &lt;/strong&gt;&lt;em&gt;R.brittoniana&lt;/em&gt; flowers were obtained from Depok, West Java, Indonesia. The flowers are extracted gradually with n-hexane, ethyl acetate, and ethanol solvents. The extracts were evaluated for anticancer activity by MTT method.&lt;strong&gt; Results: &lt;/strong&gt;IC&lt;sub&gt;50&lt;/sub&gt; values for ethanol extract, ethyl acetate extract and n-hexane extract of &lt;em&gt;R. brittoniana&lt;/em&gt; flowers are 116.55 ppm, 52.62 ppm, and 123.09 ppm, respectively, which indicating that ethanol extract has moderate anticancer activity, while ethyl acetate and n-hexane extract of &lt;em&gt;R. brittoniana&amp;nbsp;&lt;/em&gt;flowers have weak anticancer activity.&lt;strong&gt; Conclusion:&lt;/strong&gt; Ethanol, ethyl acetate, and n-hexane extract of &lt;em&gt;R.brittoniana&lt;/em&gt; flowers have a potential to become natural anti-cervical cancer.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">29</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Nadzila Anindya Tejaputri&lt;sup&gt;1&lt;/sup&gt;, Ade Arsianti&lt;sup&gt;2,3,&lt;/sup&gt;*, Fona Qorina&lt;sup&gt;1&lt;/sup&gt;, Qotrunnada Fithrotunnisa&lt;sup&gt;1&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;3&lt;/sup&gt;, Rista Putrianingsih&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Drug Development Research Cluster, Drug Discovery Division, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Surya Dwira</style></author><author><style face="normal" font="default" size="100%">Ariska TP</style></author><author><style face="normal" font="default" size="100%">Fadilah Fadilah</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Linda Erlina</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparison of Cytotoxicity between Ethyl Acetate and Ethanol Extract of White Turmeric (Kaempferia rotunda) Rhizome Extract Against HeLa Cervical Cancer Cell Activity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti cervical cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro</style></keyword><keyword><style  face="normal" font="default" size="100%">Kaempferia rotunda</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1297-1302</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Aim: &lt;/strong&gt;The aim of this study is to compare between ethanol and ethyl acetate rhizome extract of &lt;em&gt;K.rotunda &lt;/em&gt;against HeLa cervical cancer cell &lt;em&gt;in vitro. &lt;/em&gt;&lt;strong&gt;Material and Methods: &lt;/strong&gt;Methods used in this research are test the chemical compound of extracts using Thin Layer Chromatography (TLC) and phytochemical screening test, also cytotoxicity test using MTT assay. &lt;strong&gt;Result:&lt;/strong&gt; Ethyl acetate extract contains flavonoid, alkaloid, tannin, and triterpenoid, while ethanol extract have flavonoid, triterpenoid, and alkaloid. In addition, ethanol extract has strong cytotoxic activity (IC&lt;sub&gt;50&lt;/sub&gt; = 16,939 μg/ml) while ethyl acetate extract has moderate cytotoxic activity (IC&lt;sub&gt;50&lt;/sub&gt; = 127,9 μg/ml). Each of extracts showed significant results (p ≤ 0,05) although when compared between concentrations there are several concentrations that are not significant and also small coefficient of determinant values caused by various confounding factors. &lt;strong&gt;Conclusion:&lt;/strong&gt; The ethanol extract of &lt;em&gt;K.rotunda &lt;/em&gt;rhizome extract has the higher cytotoxicity activity compared to ethyl acetate extract of&lt;em&gt; K.rotunda&lt;/em&gt; rhizome extract against HeLa cervical cancer cell.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1297</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Surya Dwira&lt;sup&gt;1&lt;/sup&gt;, Ariska TP&lt;sup&gt;2&lt;/sup&gt;, Fadilah Fadilah&lt;sup&gt;1,3,&lt;/sup&gt;*, Norma Nur Azizah&lt;sup&gt;3&lt;/sup&gt;, Linda Erlina&lt;sup&gt;1&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fona Qorina</style></author><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Qotrunnada Fithrotunnisa</style></author><author><style face="normal" font="default" size="100%">NadzilaAnindya Tejaputri</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Rista Putrianingsih</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxicity of Soursop Leaves (Annona muricata) against Cervical HeLa Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Annona muricata</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Soursop</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">20-24</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Cervical cancer is the cancer with highest prevalence and the leading cause of women death in Indonesia. Current treatments available for cervical cancer are chemotherapy, radiation, surgery, and nuclear therapy. Unfortunately, these treatments still have several limitations due to serious side effects, development of resistance, and very expensive price. Therefore, it is necessary to develop effective and low-cost therapy to treat cervical cancer. One of which is by utilizing natural sources available in Indonesia such as soursop (&lt;em&gt;Annona muricata&lt;/em&gt;) leaves which has been used in folk medicine as a treatment for various diseases, including cancer. However, studies about its cytotoxicity against cervical cancer in Indonesia are still limited. &lt;strong&gt;Objective: &lt;/strong&gt;The aim of this research is to analyze the potency of &lt;em&gt;A.muricataleaves&lt;/em&gt; extracts originated from Indonesia as a novel alternative treatment for cervical cancer. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;&lt;em&gt;A.muricata&lt;/em&gt; leaves obtained from Serpong, West Java, Indonesia were grounded and macerated in three different solvents with various polarity, namely ethanol (polar solvent), ethyl acetate (semipolar solvent) and hexane (non-polar solvent). Subsequently, the extracts were diluted into 8 various concentrations. Cytotoxicity of &lt;em&gt;A.muricataleaves &lt;/em&gt;extracts against HeLa cervical cancer cells were determined by MTT assay and expressed by IC&lt;sub&gt;50 &lt;/sub&gt;value.&lt;strong&gt; Results: &lt;/strong&gt;The results showed that three extracts of &lt;em&gt;A.muricata&lt;/em&gt; have strong cytotoxicity against cervical HeLa cells. The highest cytotoxic activity was shown by etanol extract with an IC&lt;sub&gt;50&lt;/sub&gt; of 35.51 μg/mL, followed by ethyl acetate (IC&lt;sub&gt;50&lt;/sub&gt;: 5.91 μg/mL), and hexane (IC&lt;sub&gt;50&lt;/sub&gt;: 8.39 μg/mL). &lt;strong&gt;Conclusion:&lt;/strong&gt; &lt;em&gt;A.muricata &lt;/em&gt;leaves extracts are potential to be developed as a novel alternative therapy for cervical cancer.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">20</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Fona Qorina&lt;sup&gt;1&lt;/sup&gt;, Ade Arsianti&lt;sup&gt;2,3,&lt;/sup&gt;*, Qotrunnada Fithrotunnisa&lt;sup&gt;1&lt;/sup&gt;, NadzilaAnindya Tejaputri&lt;sup&gt;1&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;3&lt;/sup&gt;, Rista Putrianingsih&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Drug Development Research Cluster, Drug Discovery Division, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Qotrunnada Fithrotunnisa</style></author><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Gerry Kurniawan</style></author><author><style face="normal" font="default" size="100%">Fona Qorina</style></author><author><style face="normal" font="default" size="100%">Nadzila Anindya Tejaputri</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Cytotoxicity of Hibiscus sabdariffa Linn Extracts on A549 Lung Cancer Cell Line</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549 Lung Cancer Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Hibiscus sabdariffa Linn</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">14-19</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Lung cancer is the one of the leading causes of cancer death. However, current treatments for lung cancer are expensive and show negative side effects. Therefore, the study concerning natural anticancer from plants has intensified. &lt;em&gt;Hibiscus sabdariffa&lt;/em&gt; Linn are Indonesian herb plants which have been consumed as a drink, are known to have anticancer activity against several cancer cell lines. However, its potential cytotoxic activity on A549 lung cancer cell line is still unclear. &lt;strong&gt;Objective:&lt;/strong&gt; This study aimed to identify cytotoxic activity of &lt;em&gt;Hibiscus sabdariffa&lt;/em&gt; Linn extracts on A549 lung cancer cell line. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;&lt;em&gt;Hibiscus sabdariffa&lt;/em&gt; Linn flowers from Tangerang, province of Banten, Indonesia, were macerated in three different solvents: ethyl acetate, ethanol, and n-hexane. Afterwards, cytotoxic activity of &lt;em&gt;Hibiscus sabdariffa &lt;/em&gt;Linn extracts on A549 lung cancer cell line were evaluated using MTT assay. There were eight variety of concentration of the extracts, the experiment has been done triplicate for each concentration. The anticancer activity is expressed by IC&lt;sub&gt;50&lt;/sub&gt; value. &lt;strong&gt;Results: &lt;/strong&gt;&lt;em&gt;Hibiscus sabdariffa&lt;/em&gt; Linn extracts in ethanol, ethyl acetate, and n-hexane showed IC&lt;sub&gt;50&lt;/sub&gt; value of 374.01 μg/mL, 719.28 μg/mL, and 906.57 μg/mL respectively, in which indicated weak cytotoxic activity on A549 lung cancer cell line. &lt;strong&gt;Conclusion:&lt;/strong&gt; Ethanol, ethyl acetate, and n-hexane extracts of &lt;em&gt;Hibiscus sabdariffa&lt;/em&gt; Linn are potential to be further developed as natural anticancer agents.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">14</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Qotrunnada Fithrotunnisa&lt;sup&gt;1&lt;/sup&gt;, Ade Arsianti&lt;sup&gt;2,3,&lt;/sup&gt;*, Gerry Kurniawan&lt;sup&gt;1&lt;/sup&gt;, Fona Qorina&lt;sup&gt;1&lt;/sup&gt;, Nadzila Anindya Tejaputri&lt;sup&gt;1&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Medical Student, Department of Medical Chemistry, Faculty of Medicine University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Drug Development Research Cluster, Drug Discovery Division, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Anton Bahtiar</style></author><author><style face="normal" font="default" size="100%">Vincent Kharisma Wangsaputra</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Wilzar Fachri</style></author><author><style face="normal" font="default" size="100%">Lince Dameria Nadapdap</style></author><author><style face="normal" font="default" size="100%">Ajeng Megawati Fajrin</style></author><author><style face="normal" font="default" size="100%">Hiroki Tanimoto</style></author><author><style face="normal" font="default" size="100%">Kiyomi Kakiuchi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemical Composition and Evaluation of Marine Algal Sargassum polycystum for Antioxidant Activity and In Vitro Cytotoxicity on Hela Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cells</style></keyword><keyword><style  face="normal" font="default" size="100%">phytochemisty</style></keyword><keyword><style  face="normal" font="default" size="100%">Sargassum polycystum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">88-94</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;&lt;em&gt;Sargassum polycystum&lt;/em&gt; is one of marine algal which has a potent antioxidant anticancer activities. This research aims to investigate phytochemical composition, antioxidant activity and &lt;em&gt;in vitro &lt;/em&gt;cytotoxicity of marine algal &lt;em&gt;Sargassum polycystum &lt;/em&gt;on cervical HeLa cancer.&lt;strong&gt; Methods:&lt;/strong&gt; &lt;em&gt;Sargassum polycystum&lt;/em&gt; collected from Dompu beach, Lombok, Nusa Tenggara Barat Province, Indonesia, were extracted into organic solvent of n-hexane, ethylacetate, chloroform and ethanol, respectively. Subsequently, &lt;em&gt;Sargassum polycystum&lt;/em&gt; extracts were applied for Thin Layer Chromatography (TLC) analysis, phytochemistry test, total phenolic and total flavonoid contents, as well as for antioxidant activity test by DPPH (2,2-diphenyl-1-picrylhydrazyl) method, and in vitro cytotoxicity evaluation on HeLa cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide) assay. &lt;strong&gt;Results: &lt;/strong&gt;Phytochemical analysis of&lt;em&gt; S. polycystum&lt;/em&gt; extracts are positive for metabolites of flavonoid, steroid, tannin and glycoside. TLC analysis revealed that &lt;em&gt;S. polycystum&lt;/em&gt; extracts containing four phytochemical components. Ethylacetate extract of&lt;em&gt; S. polycystum&lt;/em&gt; showed the highest total phenolic content, and exhibited greater antioxidant activity than ethanol extract. Total phenolic and total flavonoid content in ethylacetate extract are 548.61 μg/mL and 40.06 μg /mL, respectively. Ethylacetate extract of &lt;em&gt;S. polycystum&lt;/em&gt; with IC&lt;sub&gt;50&lt;/sub&gt; value of 298.3 μg/mL is assigned to have a weak antioxidant activity against DPPH free radical. The results indicate that antioxidant activity of ethylacetate extracts of &lt;em&gt;S. polycystum&lt;/em&gt; is directly correlated with its total phenolic and flavonoid content. Moreover, &lt;em&gt;S. polycystum&lt;/em&gt; extracts demonstrated a strong anticancer activity on cervical HeLa cells with IC&lt;sub&gt;50 &lt;/sub&gt;ranging from 38.3 μg/mL to 112.8 μg/mL. &lt;strong&gt;Conclusion:&lt;/strong&gt; This work confirmed that S.polycystum are promising natural antioxidant and anti-cervical cancer agents.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">88</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ade Arsianti&lt;sup&gt;1,2,&lt;/sup&gt;*, Anton Bahtiar&lt;sup&gt;3&lt;/sup&gt;, Vincent Kharisma Wangsaputra&lt;sup&gt;4&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;2&lt;/sup&gt;, Wilzar Fachri&lt;sup&gt;5,2&lt;/sup&gt;, Lince Dameria Nadapdap&lt;sup&gt;2&lt;/sup&gt;, Ajeng Megawati Fajrin1, Hiroki Tanimoto&lt;sup&gt;6&lt;/sup&gt;, Kiyomi Kakiuchi&lt;sup&gt;6&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacology, Faculty of Pharmacy, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Medical Pharmacy, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama-cho, Ikoma, Nara, JAPAN.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Gerry Kurniawan</style></author><author><style face="normal" font="default" size="100%">Nadzila Anindya Tejaputri</style></author><author><style face="normal" font="default" size="100%">Fona Qorina</style></author><author><style face="normal" font="default" size="100%">Qotrunnada Fithrotunnisa</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Ajeng Megawati Fajrin</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemical Profile, Antioxidant Activity and Cell Line Study of Marine Red Macroalgae Eucheuma cottonii on Lung A-549 Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell line study</style></keyword><keyword><style  face="normal" font="default" size="100%">Eucheuma cottonii</style></keyword><keyword><style  face="normal" font="default" size="100%">Lung A-549 cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemical</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">276-281</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;In this century, cancer has increased in incidence and become one of the deadliest disease in the world. However, to date, lung cancer treatments are still not fully effective, quite expensive and very exhaustive for the patient. &lt;em&gt;Eucheuma cottonii&lt;/em&gt; is an abundant marine red macroalgae in Indonesia which have a potential anti-lung cancer properties. Aim of this research is to determine phytochemical profile of &lt;em&gt;Eucheuma cottonii &lt;/em&gt;extracts, as well as to evaluate its antioxidant and cytotoxic effects on Lung A-549 cancer cells.&lt;strong&gt; Methods:&lt;/strong&gt; &lt;em&gt;Eucheuma cottonii&lt;/em&gt; obtained from Sorong beach, West Papua Province, Indonesia, were extracted with three different solvents, that is ethanol, ethylacetate, and n-hexane. These three &lt;em&gt;Eucheuma cottonii &lt;/em&gt;extracts were identified for its phytochemical profiles, antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, and cytotoxic activity on lung A-549 cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. &lt;strong&gt;Results:&lt;/strong&gt; Phytochemical analysis revealed that &lt;em&gt;Eucheuma cottonii&lt;/em&gt; contains metabolites of triterpenoid and alkaloid. Antioxidant activity evaluation showed ethanol extract of &lt;em&gt;Eucheuma cottonii &lt;/em&gt;has IC&lt;sub&gt;50&lt;/sub&gt; value of 559.76 μg/mL against DPPH free radical. Whereas cytotoxicity evaluation showed that ethanol extract and ethylacetate extract of &lt;em&gt;Eucheuma cottonii&lt;/em&gt; have cytotoxic effects on Lung A-549 cancer cells, with IC&lt;sub&gt;50 &lt;/sub&gt;value of 251.73 μg/ mL and 261.41 μg/mL, respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; These results suggesting that &lt;em&gt;Eucheuma cottonii &lt;/em&gt;extract could be further developed as a natural anti-lung cancer agent.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">276</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ade Arsianti&lt;sup&gt;1,2,&lt;/sup&gt;*, Gerry Kurniawan&lt;sup&gt;3&lt;/sup&gt;, Nadzila Anindya Tejaputri&lt;sup&gt;3&lt;/sup&gt;, Fona Qorina&lt;sup&gt;3&lt;/sup&gt;, Qotrunnada Fithrotunnisa&lt;sup&gt;3&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;2&lt;/sup&gt;, Ajeng Megawati Fajrin&lt;sup&gt;1&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Anton Bahtiar</style></author><author><style face="normal" font="default" size="100%">Fadilah Fadilah</style></author><author><style face="normal" font="default" size="100%">Vincent Kharisma Wangsaputra</style></author><author><style face="normal" font="default" size="100%">Rafika Indah Paramita</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Lince Dameria Nadapdap</style></author><author><style face="normal" font="default" size="100%">Ajeng Megawati Fajrin</style></author><author><style face="normal" font="default" size="100%">Hiroki Tanimoto</style></author><author><style face="normal" font="default" size="100%">Kiyomi Kakiuchi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Synthesis, Characterization, and Cytotoxicity Evaluation of Gallic Acid Nanoparticles Towards Breast T47D Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Gallic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Nanoparticle</style></keyword><keyword><style  face="normal" font="default" size="100%">Synthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">T47D cells</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">321-327</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Gallic acid is a naturally polyphenolic acid which shows cytotoxicity against several cancer cells, as well as it displays chemo-preventive activity which is attributed to its strong apoptosis- inducing and antioxidant effects. Thus, gallic acid has become an attractive substance to be further developed due to its strong cytotoxic activity. This study aimed to synthesize gallic acid nanoparticle coating with alginate-chitosan, and evaluate its cytotoxicity against breast T47D cancer cells.&lt;strong&gt; Methods: &lt;/strong&gt;Gallic acid nanoparticle was synthesized using ionic gelation method. The yield, size and morphology of the nanoparticles were determined by UV-Vis Spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared (FTIR) spectroscopy. Cytotoxicity evaluation of gallic acid nanoparticle towards breast T47D cancer cell is carried out by MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. &lt;strong&gt;Results:&lt;/strong&gt; Spherical nanoparticles of gallic acid with the size of 100-200 nm has been successfully synthesized in 96% of yield. Compared to gallic acid (IC&lt;sub&gt;50&lt;/sub&gt;: 20.86 μg/mL) and alginate-chitosan nanoparticle (IC&lt;sub&gt;50&lt;/sub&gt;: 38.46 μg/mL), gallic acid coating with alginate-chitosan nanoparticles demonstrated higher cytotoxicity towards breast T47D cancer cells with IC&lt;sub&gt;50 &lt;/sub&gt;value of 9.03μg/mL. &lt;strong&gt;Conclusion:&lt;/strong&gt; Our results clearly confirmed that gallic acid nanoparticles coating with alginate-chitosan showed a strong cytotoxicity towards breast T47D cancer cells, which is potential to be developed as a candidate for new anti-breast cancer agent.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">321</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ade Arsianti&lt;sup&gt;1,2,&lt;/sup&gt;*, Anton Bahtiar&lt;sup&gt;3&lt;/sup&gt;, Fadilah Fadilah&lt;sup&gt;1,2&lt;/sup&gt;, Vincent Kharisma Wangsaputra&lt;sup&gt;4&lt;/sup&gt;, Rafika Indah Paramita&lt;sup&gt;1&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;2&lt;/sup&gt;, Lince Dameria Nadapdap&lt;sup&gt;2&lt;/sup&gt;, Ajeng Megawati Fajrin&lt;sup&gt;1&lt;/sup&gt;, Hiroki Tanimoto&lt;sup&gt;5&lt;/sup&gt;, Kiyomi Kakiuchi&lt;sup&gt;5 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacology, Faculty of Pharmacy, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama-cho, Ikoma, Nara, JAPAN.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Micheylla Kusumaning Dewi</style></author><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Cut Raisya Zahira Zagloel</style></author><author><style face="normal" font="default" size="100%">Yully Astika Nugrahayning Aziza</style></author><author><style face="normal" font="default" size="100%">Kartika Dwi Kurniasari</style></author><author><style face="normal" font="default" size="100%">Baiq Kirana Dyahningrum Mandasari</style></author><author><style face="normal" font="default" size="100%">Riathul Masita</style></author><author><style face="normal" font="default" size="100%">Futihati Ruhama Zulfa</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Rista Putrianingsih</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Gracillaria verrucosa</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cervical cancer cells</style></keyword><keyword><style  face="normal" font="default" size="100%">IC50 value</style></keyword><keyword><style  face="normal" font="default" size="100%">MTT Assay</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2018</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">1007-1011</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Seaweed macroalgae of &lt;em&gt;Gracilaria verrucosa &lt;/em&gt;has been known to have a potent anticancer activity, however the cytotoxicity against cervical cancer has not been explored further. &lt;strong&gt;Objective:&lt;/strong&gt; This study aims to utilize Indonesia&amp;rsquo;s marine resource which is focused on seaweed macroalgae G. verrucosa as a future anti-cervical cancer agent. &lt;strong&gt;Materials and Method:&lt;/strong&gt; Seaweed &lt;em&gt;G. verrucosa&lt;/em&gt; originated from Labuan Aji beach, Nusa Tenggara Barat, Indonesia, extracted, macerated, and fractionated into four organic solvents of different polarity, consisting of hexane, ethyl acetate, chloroform and ethanol. Then, the macroalgae extracts are diluted into 8 different concentrations. Afterwards,&lt;em&gt; in vitro&lt;/em&gt; anticancer activity evaluation of hexane, ethyl acetate, chloroform and ethanol extracts of &lt;em&gt;G. verrucosa&lt;/em&gt; against cervical HeLa cells were conducted by MTT cell proliferation assay. Triplo mechanism is also applied in this study to increase the accuracy of the results. The anticancer activity is measured using IC&lt;sub&gt;50&lt;/sub&gt; value. &lt;strong&gt;Results:&lt;/strong&gt; The four concentrated extracts &lt;em&gt;G. verrucosa &lt;/em&gt;showed cytotoxicity against cervical HeLa cells. The greatest anticancer activity is depicted by hexane extract with an IC&lt;sub&gt;50&lt;/sub&gt; of 14.94 &amp;mu;g/mL, followed by chloroform (IC&lt;sub&gt;50&lt;/sub&gt; 15.74 &amp;mu;g/mL), ethyl acetate (IC&lt;sub&gt;50&lt;/sub&gt; 16.18 &amp;mu;g/mL), and ethanol (IC&lt;sub&gt;50&lt;/sub&gt; 19.43 &amp;mu;g/mL). &lt;strong&gt;Conclusion:&lt;/strong&gt; Our results clearly indicate that hexane, ethanol, chloroform, and ethyl acetate extracts of seaweed &lt;em&gt;G. verrucosa&lt;/em&gt; can be further developed to be anti-cervical cancer agents, with hexane extract displaying the greatest cytotoxic effect.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1007</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Micheylla Kusumaning Dewi&lt;sup&gt;1&lt;/sup&gt;, Ade Arsianti&lt;sup&gt;2,3*&lt;/sup&gt;, Cut Raisya Zahira Zagloel&lt;sup&gt;1&lt;/sup&gt;, Yully Astika Nugrahayning Aziza&lt;sup&gt;1&lt;/sup&gt;, Kartika Dwi Kurniasari&lt;sup&gt;1&lt;/sup&gt;, Baiq Kirana Dyahningrum Mandasari&lt;sup&gt;1&lt;/sup&gt;, Riathul Masita&lt;sup&gt;1&lt;/sup&gt;, Futihati Ruhama Zulfa&lt;sup&gt;1&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;3&lt;/sup&gt;, Rista Putrianingsih&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Medical Student, Department of Medical Chemistry, Faculty of Medicine University of Indonesia, Depok, INDONESIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Drug Development Research Cluster, Drug Discovery Division, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ade Arsianti</style></author><author><style face="normal" font="default" size="100%">Yully Astika Nugrahayning Aziza</style></author><author><style face="normal" font="default" size="100%">Kartika Dwi Kurniasari</style></author><author><style face="normal" font="default" size="100%">Baiq Kirana Dyahningrum Mandasari</style></author><author><style face="normal" font="default" size="100%">Riathul Masita</style></author><author><style face="normal" font="default" size="100%">Futihati Ruhama Zulfa</style></author><author><style face="normal" font="default" size="100%">Micheylla Kusumaning Dewi</style></author><author><style face="normal" font="default" size="100%">Cut Raisya Zahira Zagloel</style></author><author><style face="normal" font="default" size="100%">Norma Nur Azizah</style></author><author><style face="normal" font="default" size="100%">Rista Putrianingsih</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemical Test and Cytotoxic Activity of Macroalgae Eucheuma cottonii against Cervical HeLa Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-cervical cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Eucheuma cottonii</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cell lines</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemical test</style></keyword><keyword><style  face="normal" font="default" size="100%">Seaweed</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2018</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">1012-1017</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Intoduction:&lt;/strong&gt; Marine resource of macroalgae &lt;em&gt;Eucheuma cottonii&lt;/em&gt; from East Lombok, Nusa Tenggara Barat, Indonesia, have potential as anti-cervical cancer agent. &lt;strong&gt;Objectives:&lt;/strong&gt; Finding cytotoxic activity of &lt;em&gt;Eucheuma cottonii&lt;/em&gt; hexane, ethylacetate, chloroform and ethanol extracts against cervical HeLa cells by MTT cell proliferation assay. &lt;strong&gt;Methods:&lt;/strong&gt; The extracts was tested in phytochemical and cytotoxic activity test. Phytochemical test to identify composition of secondary metabolite such as flavonoid, alkaloid, saponins, tannin, triterpenoid, steroid and glycoside. The amount of substances contained in the extract sample was analyzed by Thin Layer Chromatography (TLC). Cytotoxic activity using HeLa cells. Results: Phytochemical test of &lt;em&gt;E. cottonii&lt;/em&gt; extracts showed the positive result for metabolite of flavonoid, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, n-hexane, chloroform, and ethyl acetate extracts of &lt;em&gt;E. cottonii&lt;/em&gt; exhibited a strong cytotoxic activity against cervical HeLa cells with IC&lt;sub&gt;50&lt;/sub&gt; of 7.54 &amp;mu;g/mL, 5.73 &amp;mu;g /mL, 4.82 &amp;mu;g /mL and 4.34 &amp;mu;g / mL, respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; The results suggest that macroalgae &lt;em&gt;Eucheuma cottonii&lt;/em&gt; could be used as a new anti-cervical cancer&amp;rsquo;s candidate.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1012</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Ade Arsianti&lt;sup&gt;1,2,*&lt;/sup&gt;, Yully Astika Nugrahayning Aziza&lt;sup&gt;3&lt;/sup&gt;, Kartika Dwi Kurniasari&lt;sup&gt;3&lt;/sup&gt;, Baiq Kirana Dyahningrum Mandasari&lt;sup&gt;3&lt;/sup&gt;, Riathul Masita&lt;sup&gt;3&lt;/sup&gt;, Futihati Ruhama Zulfa&lt;sup&gt;3&lt;/sup&gt;, Micheylla Kusumaning Dewi&lt;sup&gt;3&lt;/sup&gt;, Cut Raisya Zahira Zagloel&lt;sup&gt;3&lt;/sup&gt;, Norma Nur Azizah&lt;sup&gt;2&lt;/sup&gt;, Rista Putrianingsih&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6 Jakarta 10430, INDONESIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Medical Student, Faculty of Medicine University of Indonesia, Depok, INDONESIA.&lt;/p&gt;</style></auth-address></record></records></xml>