<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dina M Eskander</style></author><author><style face="normal" font="default" size="100%">Ezzel -Din A El-Khrisy</style></author><author><style face="normal" font="default" size="100%">Mary H Grace</style></author><author><style face="normal" font="default" size="100%">Marian Nabil</style></author><author><style face="normal" font="default" size="100%">Mahmoud I Nassar</style></author><author><style face="normal" font="default" size="100%">Marwa M Mounier</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Investigation of Secondary Metabolites and Cytotoxicity of Jacquemontia pentantha (Jacq.)</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytotoxic activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Enzyme-Linked Immunosorbent Assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Jacquemontia pentantha</style></keyword><keyword><style  face="normal" font="default" size="100%">MTT Assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Sterols</style></keyword><keyword><style  face="normal" font="default" size="100%">Terpenes</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">July 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">718-723</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; The aim of this study is to isolate and identify sterols and terpenes from the chloroform/methanol extract (3:1) of aerial parts of &lt;em&gt;Jacquemontia pentantha&lt;/em&gt; (Jacq.) and evaluation of cytotoxic activity of crude extract and phytol for the first time from this plant. &lt;strong&gt;Methods:&lt;/strong&gt; Different chromatographic techniques for the aerial parts of &lt;em&gt;Jacquemontia pentantha&lt;/em&gt; extract were used resulting in isolation of eight compounds. Their structures were elucidated by spectroscopic methods including&lt;sup&gt; 1&lt;/sup&gt;HNMR, &lt;sup&gt;13&lt;/sup&gt;CNMR, EI/MS spectrometry and by comparing their data with those reported in the literature. The cytotoxicity was evaluated using MTT assay. The mode of action of the extract was predicted by using Enzyme-linked Immunosorbent Assay Kit for Tubulin beta (TUBb). &lt;strong&gt;Results: &lt;/strong&gt;Eight compounds for the first time from this plant were identified as Palmitic acid (1), Phytol (major) (2), Stigmast-4-en- 3-one (3), mixture of α-amyrin (4) and β–amyrin (5), 1,6,10,14,18,22-Tetracosahexaen-3- ol,2,6,10,15,19,23-hexamethyl (all-E) (6) and mixture of α– amyrin acetate (7) and β-amyrin acetate (8). The extract showed potent cytotoxic activity on MCF-7 breast carcinoma cell line as well as HCT-116 colon carcinoma cell line at different concentrations (100-6.25 ug/ml) with IC&lt;sub&gt;50&lt;/sub&gt; (21.8 ± 0.9) and (40.9 ± 1.3) respectively. Phytol showed potent cytotoxic activity on MCF-7 cell line at different concentrations (100-12.5 ug/ml) with IC&lt;sub&gt;50&lt;/sub&gt; (60 ± 2.4), while it had no cytotoxic effect on HCT-116 cell line. The extract showed significant TUBb polymerization inhibition activity. &lt;strong&gt;Conclusion: &lt;/strong&gt;The extract of aerial parts of &lt;em&gt;Jacquemontia pentantha &lt;/em&gt;(Jacq.) and also phytol compound has cytotoxic activity due to the presence of phytochemicals such as sterols and terpenes.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">718</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Dina M Eskander&lt;sup&gt;1,*&lt;/sup&gt;, Ezzel -Din A El-Khrisy&lt;sup&gt;1&lt;/sup&gt;, Mary H Grace&lt;sup&gt;2&lt;/sup&gt;, Marian Nabil&lt;sup&gt;1&lt;/sup&gt;, Mahmoud I Nassar&lt;sup&gt;1&lt;/sup&gt;, Marwa M Mounier&lt;sup&gt;3&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Chemistry of Natural Compounds Department, National Research Centre, Dokki, 12622 Cairo, EGYPT.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacognosy, Drug Bioassay-Cell Culture Laboratory, National Research Centre, Dokki, 12622 Cairo, EGYPT.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Marian Nabil</style></author><author><style face="normal" font="default" size="100%">Neveen S Ghaly</style></author><author><style face="normal" font="default" size="100%">Iman AA Kassem</style></author><author><style face="normal" font="default" size="100%">Mary H Grace</style></author><author><style face="normal" font="default" size="100%">Farouk R Melek</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Two Triterpenoid Saponins with alpha-glucosidase Inhibitory Activity from Harpullia pendula Seed Extract</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Harpullia pendula</style></keyword><keyword><style  face="normal" font="default" size="100%">Sapindaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Triterpenoid saponins</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">1386-1390</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;Harpullia pendula &lt;/em&gt;Planch (family Sapindaceae) is a small to medium rainforest tree native to Australia. &lt;strong&gt;Objective: &lt;/strong&gt;This study aims to isolate triterpenoid saponins from &lt;em&gt;H. pendula &lt;/em&gt;and test them as α-glucosidase inhibitors. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;The saponin compounds were obtained using variable chromatographic techniques and characterized by spectral analysis. &lt;strong&gt;Results:&lt;/strong&gt; Two new triterpenoid saponins were obtained as an inseparable mixture from &lt;em&gt;H. pendula&lt;/em&gt; methanolic seed extract. Their structures were determined as 3-O-β-D-glucopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-βD-glucuronopyranosyl22-OangeloylA1- barrigenol and 3-O-β-D-galactopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-β-Dglucuronopyranosyl 22-O-(2-methylbutyroyl)-A1 barrigenol, respectively. The triterpene part 22-O-(2-methyl butyroyl) A1-barrigenol has never been characterized before. The α- glucosidase inhibitory activity of the two saponin mixture was evaluated &lt;em&gt;invitro&lt;/em&gt; and proved to exhibit strong activity with IC&lt;sub&gt;50&lt;/sub&gt; value equals to 13.3 ± 5.0 ppm and IC&lt;sub&gt;90 &lt;/sub&gt;value equals to 21.5 ± 8.0 ppm. &lt;strong&gt;Conclusion: &lt;/strong&gt;Two new saponins were characterized from their mixture and found to exhibit α-glucosidase inhibitory activity.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1386</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Marian Nabil, Neveen S. Ghaly, Iman A.A. Kassem, Mary H. Grace and Farouk R. Melek* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Chemistry of Natural Compounds Department, National Research Centre, Dokki,12622, Giza, EGYPT.&lt;/p&gt;
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