<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sri Ningsih</style></author><author><style face="normal" font="default" size="100%">Fifit Juniarti</style></author><author><style face="normal" font="default" size="100%">Idah Rosidah</style></author><author><style face="normal" font="default" size="100%">Adam Arditya Fajriawan</style></author><author><style face="normal" font="default" size="100%">Kurnia Agustini</style></author><author><style face="normal" font="default" size="100%">Syofi Rosmalawati</style></author><author><style face="normal" font="default" size="100%">Agung Eru Wibowo</style></author><author><style face="normal" font="default" size="100%">Erliana Sasikirana</style></author><author><style face="normal" font="default" size="100%">Wahono Sumaryono</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Study of the Effect of Lampeni (Ardisia humilis Vahl.) Planting Condition toward the Alpha-glucosidase Inhibition Activity in vitro</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alpha-glucosidase</style></keyword><keyword><style  face="normal" font="default" size="100%">Ardisia humilis Vahl.</style></keyword><keyword><style  face="normal" font="default" size="100%">Lampeni</style></keyword><keyword><style  face="normal" font="default" size="100%">Open-air</style></keyword><keyword><style  face="normal" font="default" size="100%">Shedding house</style></keyword><keyword><style  face="normal" font="default" size="100%">total phenolic</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">377-385</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;The quality of a medicinal plant is influenced by agronomic conditions and harvesting time. &lt;strong&gt;Objective: &lt;/strong&gt;This study aimed to evaluate the effect of planting method (openair (OA) and shedding house (SH)) and harvesting time (2, 4, 6 months) of Lampeni (Ardisia humilis Vahl.) toward the inhibitory activity of alpha-glucosidase. &lt;strong&gt;Methods: &lt;/strong&gt;The Lampeni seedling were placed under controlled light conditions (SH) and on direct sun exposure (OA). Harvesting of the leaves was carried out at the age of 2, 4, and 6 months after plantation (2m, 4m, and 6m). Each leaves dry powder was refluxed with methanol 70% and followed by liquid-liquid partition using n-hexane, ethyl acetate (EtOAc), and water. All samples were evaluated toward inhibition of the alpha-glucosidase enzyme &lt;em&gt;in vitro&lt;/em&gt;. Total phenol levels were determined using Folin-Ciocalteu reagent. &lt;strong&gt;Results: &lt;/strong&gt;The results showed that EtOAc fractions of both plantation techniques exhibited the highest inhibition of alpha-glucosidase. The highest activity was demonstrated by the 4m-OA-EtOAc fraction (IC&lt;sub&gt;50&lt;/sub&gt;, 93.50 ppm) and followed by the 6m-OA-EtOAc fraction (IC&lt;sub&gt;50&lt;/sub&gt;, 98.13 ppm). Based on the kinetic study, the inhibition type of the two most active samples were categorized as a non-competitive type. Total phenolic contents were decreased in the following order: 6m-SH-EtOAc &amp;gt; 4m-OA-EtOAc &amp;gt; 6m-OAEtOAc &amp;gt; 4m-SH-EtOAc fraction. It was shown that there was no positive correlation between the strength of inhibition with total phenolic content. &lt;strong&gt;Conclusions:&lt;/strong&gt; This study concluded that Lampeni at open-air plantation harvested on fourth months demonstrated the highest alpha-glucosidase inhibitory activity, although there was no positive correlation between the inhibition activity and phenolic content.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">377</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Sri Ningsih&lt;sup&gt;1,&lt;/sup&gt;*, Fifit Juniarti&lt;sup&gt;1&lt;/sup&gt;, Idah Rosidah&lt;sup&gt;1&lt;/sup&gt;, Adam Arditya Fajriawan&lt;sup&gt;1&lt;/sup&gt;, Kurnia Agustini&lt;sup&gt;1&lt;/sup&gt;, Syofi Rosmalawati&lt;sup&gt;2&lt;/sup&gt;, Agung Eru Wibowo&lt;sup&gt;2&lt;/sup&gt;, Erliana Sasikirana&lt;sup&gt;3&lt;/sup&gt;, Wahono Sumaryono&lt;sup&gt;3&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Center for Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology. Laptiab building, Puspiptek Serpong Area, South Tangerang, Banten, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Laboratory of Biotechnology, Agency for the Assessment and Application of Technology, Puspiptek Serpong Area, South Tangerang, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Pharmacy, Pancasila University. Srengseng Sawah Street, South Jakarta, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Novi Yantih</style></author><author><style face="normal" font="default" size="100%">Alfadella Methananda</style></author><author><style face="normal" font="default" size="100%">Yahdiana Harahap</style></author><author><style face="normal" font="default" size="100%">Wahono Sumaryono</style></author><author><style face="normal" font="default" size="100%">Lestari Rahayu</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Validation of High-Performance Liquid Chromatography for Determination of Bromelain in Pineapple (Ananas comosus (L) Merr) Water</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bromelain</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Pineapple water</style></keyword><keyword><style  face="normal" font="default" size="100%">Storage</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">901-906</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective: &lt;/strong&gt;The aim of the present study was to validate HPLC method for analysis bromelain levels in pineapple water and application that method to determine the storage time of pineapple water. &lt;strong&gt;Methods:&lt;/strong&gt; The reversed phase of HPLC method was tested and optimized before it is validated. The composition and the flow rate of the mobile phase is the optimized parameter. The analytical parameters validated were detection limits, linearity, accuracy and precision. Pineapple water was stored for 8 h at 10ºC and bromelain was determined using the validated HPLC method.&lt;strong&gt; Result: &lt;/strong&gt;The optimum mobile phase composition was methanolwater (70:30) with a flow rate of 1 ml/min. The detector concentration-response was linear with coefficient of determination of 0.993. The accuracy of HPLC method at a recovery of 1 and 2% bromelain was 106.37 ± 1.94% and 98.12 ± 1.29% (n = 5), respectively. The precision, expressed as the coefficients of variation (CV), at 1 and 2% bromelain were 1.83 and 1.32% (n = 5), respectively. Bromelain level at zero time was 81.53%. After storage for 8 h at 10ºC, bromelain levels in pineapple juice appeared to decrease not statistically significant (p &amp;gt; 0.05), with to mean value of 78.46 ± 2.88%. &lt;strong&gt;Conclusion:&lt;/strong&gt; The HPLC method developed was valid to analyze accurately concentrations of bromelain in pineapple water and it can be used to study the shelf life of pineapple water based on bromelain content. The bromelain content in pineapple juice was not statistically significantly different after 8 h storage at 10°C.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">901</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Novi Yantih&lt;sup&gt;1,&lt;/sup&gt;*, Alfadella Methananda&lt;sup&gt;1&lt;/sup&gt;, Yahdiana Harahap&lt;sup&gt;2&lt;/sup&gt;, Wahono Sumaryono&lt;sup&gt;1&lt;/sup&gt;, Lestari Rahayu&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, INDONESIA.&lt;/p&gt;
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