<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rifki Febriansah</style></author><author><style face="normal" font="default" size="100%">Titi Komalasari</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Co-Chemotherapeutic Effect of Ageratum conyzoides L. Chloroform Fraction and 5-Fluorouracil on Hela Cell Line</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ageratum conyzoides L.</style></keyword><keyword><style  face="normal" font="default" size="100%">Bcl-XL protein</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxic assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Nobiletin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">913-918</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; This study was to determine the co-chemotherapeutic effect of the chloroform fraction of bandotan (&lt;em&gt;Ageratum conyzoides&lt;/em&gt; L.) (CFB) and its combination with 5-Fluorouracil (5-FU) by&lt;em&gt; in vitro&lt;/em&gt; and&lt;em&gt; in silico &lt;/em&gt;assay. &lt;strong&gt;Methods:&lt;/strong&gt; Ethanolic extract of bandotan were fractionated with chloroform. Thin Layer Chromatography (TLC) used to identify active compound and &lt;em&gt;in vitro&lt;/em&gt; study with MTT Assay to determine the viability of HeLa cells after extract treatment.&lt;em&gt; Molecular docking&lt;/em&gt; used Autodock Vina for &lt;em&gt;in silico&lt;/em&gt; study to visualize molecular interaction and affinity between nobiletin and 5-FU with Bcl-XL protein. &lt;strong&gt;Results:&lt;/strong&gt; The result of TLC for CFB showed the Rf value of 0.75, it has the similar value with quersetin standard and indicated that CFB contains flavonoid compound. The &lt;em&gt;Molecular docking&lt;/em&gt; had ΔG for nobiletin and 5-FU were -8.0 and -4.7 kcal/mol, respectively. This result showed that the affinity of nobiletin with Bcl-XL protein higher than 5-FU. Single cytotoxic assay of CFB and 5-FU showed the IC&lt;sub&gt;50&lt;/sub&gt; value of 30 μg/ml and 45 μg/ml, respectively. Combination assay of CFB and 5-FU showed the CI value of 0.36, meaning the presence of synergistic effects. &lt;strong&gt;Conclusion:&lt;/strong&gt; CFB has a positive effect to inhibit viability of HeLa cervical cancer cells and potential to develop as co-chemotherapy agent with 5-FU.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">913</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Rifki Febriansah*, Titi Komalasari&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;School of Pharmacy, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta, INDONESIA.&lt;/p&gt;
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