<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kartini Kartini</style></author><author><style face="normal" font="default" size="100%">Ervina Rustiana Dewi</style></author><author><style face="normal" font="default" size="100%">Fandi Achmad</style></author><author><style face="normal" font="default" size="100%">Nikmatul Ikhrom Eka Jayani</style></author><author><style face="normal" font="default" size="100%">Mochammad Arbi Hadiyat</style></author><author><style face="normal" font="default" size="100%">Christina Avanti</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Thin Layer Chromatography Fingerprinting and Clustering of Orthosiphon stamineus Benth. from Different Origins</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Geographical origin</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbal medicine</style></keyword><keyword><style  face="normal" font="default" size="100%">Marker</style></keyword><keyword><style  face="normal" font="default" size="100%">Principal Component Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quality</style></keyword><keyword><style  face="normal" font="default" size="100%">TLC</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">79-87</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; has been widely used across Asian countries for the treatment of various diseases. The quality of herbal medicine determine its safety as well as efficacy; and geographical origin is important factor contributing on the quality of herb and its products. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Thin Layer Chromatography (TLC) method combined with chemometric, Principal Component Analysis (PCA), has been employed to evaluate the quality of &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; leaves collected from eleven origins in Indonesia.&lt;strong&gt; Results:&lt;/strong&gt; The results showed that mobile phase suitable for &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; was chloroform, dichloromethane, ethyl acetate (7:4:1). The method used has met the requirements of TLC system stability and precision. TLC-fingerprints analyzed with chemometrics showed an ability to discriminate &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; from various origins. PCA score plot of the first two principal components (PC) clearly distinguished 3 clusters of samples, whereas the loading plot of the first two PC showed that compounds with the Rf values of 0.0-0.1, 0.1-0.2, 0.2-0.3, and 0.9-1.0 are the most important compounds for clustering of samples. &lt;strong&gt;Conclusions:&lt;/strong&gt; TLCfingerprint combined with the PCA was able to discriminate among the leaves of &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; originated from various locations. TLC-fingerprints analyzed with chemometrics can be used as an alternative of marker-oriented method to evaluate the quality of &lt;em&gt;Orthosiphon stamineus.&lt;/em&gt;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">79</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Kartini Kartini&lt;sup&gt;1,&lt;/sup&gt;*, Ervina Rustiana Dewi&lt;sup&gt;1&lt;/sup&gt;, Fandi Achmad&lt;sup&gt;1&lt;/sup&gt;, Nikmatul Ikhrom Eka Jayani&lt;sup&gt;1&lt;/sup&gt;, Mochammad Arbi Hadiyat&lt;sup&gt;2&lt;/sup&gt;, Christina Avanti&lt;sup&gt;3&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Industrial Engineering, Faculty of Engineering, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmaceutic, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kartini Kartini</style></author><author><style face="normal" font="default" size="100%">Christina Avanti</style></author><author><style face="normal" font="default" size="100%">Chutima Phechkrajang</style></author><author><style face="normal" font="default" size="100%">Omboon Vallisuta</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antioxidant Activity, HPTLC Fingerprint and Discriminant Analysis of Plantago major Leaves from Diverse Origins in Indonesia</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chemometrics</style></keyword><keyword><style  face="normal" font="default" size="100%">Flavonoids</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbal medicines</style></keyword><keyword><style  face="normal" font="default" size="100%">Pattern-oriented</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenolics</style></keyword><keyword><style  face="normal" font="default" size="100%">PLSDA</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">1483-1489</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Plantago major &lt;/em&gt;L. (&lt;em&gt;Plantaginaceae&lt;/em&gt;) is a perennial herb having contribution to the folk medicine all around the world, including Indonesia with wide geographical distribution. Plant materials origin is one factor that significantly influences the quality of herbal medicines. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; In this paper, High-Performance Thin Layer Chromatography (HPTLC) method using pattern-oriented approach has been employed to evaluate the quality of &lt;em&gt;Plantago major&lt;/em&gt; leaves collected from seven origins in Indonesia. To differentiate the antioxidant capacities of those plant materials, the crude extracts were tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolics, and total flavonoids assay methods. &lt;strong&gt;Results:&lt;/strong&gt; The results showed that radical scavenging activity, total phenolics, and total flavonoids of plant material from seven origins were significantly different. Moreover, HPTLC fingerprints analyzed with chemometrics showed an ability to discriminate the leaves samples from various origins as well as detect chemicals responsible for discrimination. Two models using principal component analysis (PCA) and partial least squares (PLS-DA) were built in chemometrics test. The PCA model was able to describe the studied samples by using four principal components with a value of explained variance of 95%, whereas PLS-DA model accurately classified the leaves samples with prediction ability of 100%. In the PCA, loading plot of the first PC showed that peaks number 10 and 12 are the most important peaks for clustering of the samples. &lt;strong&gt;Conclusions: &lt;/strong&gt;&lt;em&gt;Plantago major &lt;/em&gt;collected from different origins revealed different radical scavenging activity and concentration of total phenolics as well as total flavonoids. HPTLC fingerprints coupled with chemometrics analysis can be used as an alternative to marker-oriented method for the quality control of &lt;em&gt;Plantago major&lt;/em&gt;.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1483</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Kartini Kartini&lt;sup&gt;1,&lt;/sup&gt;*, Christina Avanti&lt;sup&gt;2&lt;/sup&gt;, Chutima Phechkrajang&lt;sup&gt;3&lt;/sup&gt;, Omboon Vallisuta&lt;sup&gt;4&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmaceutic, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Road, Ratchathewi, Bangkok 10400, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Road, Ratchathewi, Bangkok 10400, THAILAND.&lt;/p&gt;
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