<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Srikalyani V</style></author><author><style face="normal" font="default" size="100%">Ilango K</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Chemical Fingerprint by HPLC-DAD-ESI-MS, GC-MS Analysis and Anti-Oxidant Activity of Manasamitra Vatakam: A Herbomineral Formulation</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Classical formulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Diffusion and dilution methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy metals</style></keyword><keyword><style  face="normal" font="default" size="100%">MIC</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemicals</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">115-123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Manasamitra Vatakam is a classical ayurvedic herbo mineral formulation used for the treatment of neurodegerative properties and epileptic disorders. The wide range mixture of herbal extracts and minerals were used in the formulation. &lt;strong&gt;Aim:&lt;/strong&gt; The aim of the study implies in performing the chemo-profiling, chromatographic fingerprint analysis by HPLC-DAD-ESI-MS for the selected formulations of Manasamitra Vatakam followed by the identification of bioactive compounds by Gas Chromatography – Mass Spectrometric (GC-MS) analysis, to evaluate the diffusion and dilution methods for the determination of anti-bacterial activity in the methanolic extracts of Manasamitra Vatakam (MMV). &lt;strong&gt;Materials and Methods: &lt;/strong&gt;The antibacterial activity was performed by both diffusion and dilution methods whereas the antioxidant activity was performed by free radical scavenging of 2,2-diphenyl-1-picrylhydrazy and hydrogen peroxide scavenging assay method. &lt;strong&gt;Results:&lt;/strong&gt; The estimation of bioactive constituents showed positive results by qualitative analysis. Antibacterial activity of MMV was evaluated against two-gram positive &lt;em&gt;Staphylococcus aureus&lt;/em&gt; and &lt;em&gt;Bacillus cereus&lt;/em&gt;, two gram negative &lt;em&gt;Escherichia coli &lt;/em&gt;and&lt;em&gt; Klebsiella pneumonia &lt;/em&gt;by disk diffusion (0.078-10μg mL&lt;sup&gt;-1&lt;/sup&gt;), broth dilution (0.078-10μg mL&lt;sup&gt;-1&lt;/sup&gt;) and broth micro dilution method (0.39-50μg mL&lt;sup&gt;-1&lt;/sup&gt;) respectively. The bioactive constituents were analysed by GC-MS analysis for the methanolic extract of the formulation. &lt;strong&gt;Conclusion:&lt;/strong&gt; To conclude, the formulation was found abundant with phenolic and flavonoid compounds by HPLC-ESI-MS analysis, the bioactive compounds identified are responsible for the anti-bacterial activity. The broth microdilution method performed by resazurin method was observed as the fast screening, sensitive and accurate method for the quantitative determination of antibacterial activity.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">115</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Srikalyani V&lt;sup&gt;1&lt;/sup&gt;, Ilango K&lt;sup&gt;1,2,&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Division of Analytical Chemistry, Interdisciplinary Institute of Indian System of Medicine (IIISM), SRM Institute of Science and Technology, Kattankulathur- 603 203, Kancheepuram (Dt), Tamil Nadu, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur-603 203, Kancheepuram (Dt), Tamil Nadu, INDIA.&lt;/p&gt;
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