<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kartini Kartini</style></author><author><style face="normal" font="default" size="100%">Ervina Rustiana Dewi</style></author><author><style face="normal" font="default" size="100%">Fandi Achmad</style></author><author><style face="normal" font="default" size="100%">Nikmatul Ikhrom Eka Jayani</style></author><author><style face="normal" font="default" size="100%">Mochammad Arbi Hadiyat</style></author><author><style face="normal" font="default" size="100%">Christina Avanti</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Thin Layer Chromatography Fingerprinting and Clustering of Orthosiphon stamineus Benth. from Different Origins</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Geographical origin</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbal medicine</style></keyword><keyword><style  face="normal" font="default" size="100%">Marker</style></keyword><keyword><style  face="normal" font="default" size="100%">Principal Component Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quality</style></keyword><keyword><style  face="normal" font="default" size="100%">TLC</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February  2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">79-87</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; has been widely used across Asian countries for the treatment of various diseases. The quality of herbal medicine determine its safety as well as efficacy; and geographical origin is important factor contributing on the quality of herb and its products. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Thin Layer Chromatography (TLC) method combined with chemometric, Principal Component Analysis (PCA), has been employed to evaluate the quality of &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; leaves collected from eleven origins in Indonesia.&lt;strong&gt; Results:&lt;/strong&gt; The results showed that mobile phase suitable for &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; was chloroform, dichloromethane, ethyl acetate (7:4:1). The method used has met the requirements of TLC system stability and precision. TLC-fingerprints analyzed with chemometrics showed an ability to discriminate &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; from various origins. PCA score plot of the first two principal components (PC) clearly distinguished 3 clusters of samples, whereas the loading plot of the first two PC showed that compounds with the Rf values of 0.0-0.1, 0.1-0.2, 0.2-0.3, and 0.9-1.0 are the most important compounds for clustering of samples. &lt;strong&gt;Conclusions:&lt;/strong&gt; TLCfingerprint combined with the PCA was able to discriminate among the leaves of &lt;em&gt;Orthosiphon stamineus&lt;/em&gt; originated from various locations. TLC-fingerprints analyzed with chemometrics can be used as an alternative of marker-oriented method to evaluate the quality of &lt;em&gt;Orthosiphon stamineus.&lt;/em&gt;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">79</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Kartini Kartini&lt;sup&gt;1,&lt;/sup&gt;*, Ervina Rustiana Dewi&lt;sup&gt;1&lt;/sup&gt;, Fandi Achmad&lt;sup&gt;1&lt;/sup&gt;, Nikmatul Ikhrom Eka Jayani&lt;sup&gt;1&lt;/sup&gt;, Mochammad Arbi Hadiyat&lt;sup&gt;2&lt;/sup&gt;, Christina Avanti&lt;sup&gt;3&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Industrial Engineering, Faculty of Engineering, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmaceutic, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut Road, Surabaya 60293, INDONESIA.&lt;/p&gt;
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