<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Han-jing Yan</style></author><author><style face="normal" font="default" size="100%">Meng-ling He</style></author><author><style face="normal" font="default" size="100%">Wei-Jian Huang</style></author><author><style face="normal" font="default" size="100%">Dong-mei Li</style></author><author><style face="normal" font="default" size="100%">Xiao-fang Yu</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agrobacterium rhizogenes</style></keyword><keyword><style  face="normal" font="default" size="100%">ATCC15834.</style></keyword><keyword><style  face="normal" font="default" size="100%">C58C1</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant regeneration</style></keyword><keyword><style  face="normal" font="default" size="100%">Pogostemon cablin.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">09/2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">50-55</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;An efficient transformation system for the medicinal and aromatic plant, &lt;em&gt;Pogostemon cablin &lt;/em&gt;Benth was developed by using &lt;em&gt;Agrobacterium rhizogenes &lt;/em&gt;ATCC15834 and C58C1. Hairy roots formed directly from the cut edges of leaf explants after infection for 2 days. The highest frequency of leaf explant transformation by &lt;em&gt;A. rhizogenes&lt;/em&gt; ATCC15834 and C58C1 were 83.3% and 80.5% after pre culture about 2 days and infection by the bacterium containing 15 mg l&lt;sup&gt;-1 &lt;/sup&gt;acetosyringone about 25 min. The PCR ampliﬁcation showed that rolB genes of Ri plasmid of &lt;em&gt;A. rhizogenes&lt;/em&gt; were integrated and expressed into the genome of transformed hairy roots. The optimum medium for callus induction of hairy roots consisted of 2.0 mg l&lt;sup&gt;-1&lt;/sup&gt; benzyladenine (BA) and 0.1 mg l&lt;sup&gt;-1&lt;/sup&gt; a-naphthaleneacetic acid (NAA); while optimum medium for adventitious shoot regeneration from these cultures consisted of 0.1 mg l&lt;sup&gt;-1&lt;/sup&gt; BA and 0.1 mg l&lt;sup&gt;-1&lt;/sup&gt; NAA. Adventitious shoots could be rooted on 1/2MS. PCR analysis conﬁrmed that rol genes of TL-DNA of Ri plasmid was integrated with at least three copies into the genome of hairy roots-regenerated &lt;em&gt;P. cablin&lt;/em&gt; plants. The results presented provide a possibility for breeding of a new cultivar of &lt;em&gt;P. cablin.&lt;/em&gt;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">50</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Han-Jing Yan&lt;sup&gt;1*&lt;/sup&gt;, Meng-ling He&lt;sup&gt;1&lt;/sup&gt;, Wei-Jian Huang&lt;sup&gt;1&lt;/sup&gt;, Dong-mei Li&lt;sup&gt;2&lt;/sup&gt; and Xiao-fang Yu&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou Guangdong, 510006 China.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Shunde Polytechnic, Foshan 528300, China.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;School of foreign languages, Guangdong Pharmaceutical University, Guangzhou Guangdong, 510006 China.&lt;/p&gt;</style></auth-address></record></records></xml>