<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tedjo Narko</style></author><author><style face="normal" font="default" size="100%">Marlia Singgih Wibowo</style></author><author><style face="normal" font="default" size="100%">Sophi Damayanti</style></author><author><style face="normal" font="default" size="100%">Indra Wibowo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Acute Toxicity Tests of Fermented Robusta Green Coffee Using Zebrafish Embryos (Danio rerio)</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Green coffee</style></keyword><keyword><style  face="normal" font="default" size="100%">Kombucha</style></keyword><keyword><style  face="normal" font="default" size="100%">Toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Zebrafish embryos</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">485-492 </style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Green coffee beans are coffee beans of coffee fruit that have not yet been roasted. The use of green coffee beans as a weight-loss agent has been widely used worldwide, but nowadays there is a new way to enjoy coffee by adding kombucha culture to it, or what is known as kombucha coffee. The development of this fermented product preparation still requires a more in-depth study, one of which is related to the toxicity of the kombucha green coffee preparation. &lt;strong&gt;Objective:&lt;/strong&gt; This research was aimed to determine LC&lt;sub&gt;50 &lt;/sub&gt;values for robusta green coffee fermented with kombucha culture using zebrafish (&lt;em&gt;Danio rerio&lt;/em&gt;) embryos using an &lt;em&gt;in vivo &lt;/em&gt;method. &lt;strong&gt;Methods: &lt;/strong&gt;This study observed the development of 20 zebrafish embryos administered one of five different concentrations of kombucha coffee preparation 24 hours up to 96 hours, with the experiment repeated three times. The percentage of embryo deaths was observed and analyzed using a probit model for LC&lt;sub&gt;50&lt;/sub&gt; concentration and analyzed using IBM SPSS Ver 23. &lt;strong&gt;Results:&lt;/strong&gt; An LC&lt;sub&gt;50&lt;/sub&gt; for kombucha green coffee of 1294.29 ppm was obtained. Statistical tests on the concentration of kombucha coffee extracts were not significant differences with p-values &amp;gt; 0.05.&lt;strong&gt; Conclusion&lt;/strong&gt;: The LC&lt;sub&gt;50&lt;/sub&gt; of robusta green coffee extract using zebrafish embryos of 1294.29 ppm included in the safe category.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">485 </style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Tedjo Narko&lt;sup&gt;1,2&lt;/sup&gt;, Marlia Singgih Wibowo&lt;sup&gt;1,&lt;/sup&gt;*, Sophi Damayanti&lt;sup&gt;1&lt;/sup&gt;, Indra Wibowo&lt;sup&gt;1 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Bandung Institute of Technology, Jalan Ganesha 10 Bandung, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Poltekkes TNI AU, Jalan Ciumbuleuit No.203 Bandun0g, INDONESIA.&lt;/p&gt;
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