<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nanthakarn Woottisin</style></author><author><style face="normal" font="default" size="100%">Sumet Kongkiatpaiboon</style></author><author><style face="normal" font="default" size="100%">Sophida Sukprasert</style></author><author><style face="normal" font="default" size="100%">Korbtham Sathirakul</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development and Validation of Stability Indicating HPLC Method for Determination of Caffeic Acid, Vitexin and Rosmarinic Acid in Thunbergia laurifolia Leaf Extract</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Force degradation</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenolic compound</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemical screening</style></keyword><keyword><style  face="normal" font="default" size="100%">Stabilityindicating method</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">611-618</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;em&gt;Thunbergia laurifolia &lt;/em&gt;has been a popular herb used in Thai traditional medicine for detoxification and as antipyretic. It contains rosmarinic acid (RA), caffeic acid (CA) and vitexin as major compounds. In order to control the herbal quality, the stability indicating high-performance liquid chromatography (HPLC) was developed and validated. The stability study of compounds in &lt;em&gt;T. laurifolia&lt;/em&gt; leaf extract was investigated. The chromatographic separation was performed using a reversed-phase C18 column and mobile phase consisted of 0.5% acetic acid and methanol using a gradient elution with 1.0 mL/min flow rate. The detection wavelength was set at 330 nm. The method was validated for its linearity, precision, accuracy, limit of detection and limit of quantitation. Forced degradation of three compounds in extract showed that they were stable in oxidative condition, but highly labile under alkaline hydrolytic conditions. All three compounds in &lt;em&gt;T. laurifolia &lt;/em&gt;leaf extract were stable at room temperature at least 3 months while a remarkable decrease of RA, vitexin and CA in the extract were found in accelerated condition. This finding could be applied for predicting the storage recommendation and expiry of&lt;em&gt; T. laurifolia&lt;/em&gt; extract and its related pharmaceutical products.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">611</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Nanthakarn Woottisin&lt;sup&gt;1&lt;/sup&gt;, Sumet Kongkiatpaiboon&lt;sup&gt;2&lt;/sup&gt;, Sophida Sukprasert&lt;sup&gt;1,3,&lt;/sup&gt;*, Korbtham Sathirakul&lt;sup&gt;4 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Division of Integrative Medicine, Chulabhorn International College of Medicine, Thammasat University (Rangsit Campus), Pathum Thani 12120, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathum Thani 12120, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen 40002, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, THAILAND.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sumet Kongkiatpaiboon</style></author><author><style face="normal" font="default" size="100%">Piyanuch Rojsanga,</style></author><author><style face="normal" font="default" size="100%">Virote Pattarajinda,</style></author><author><style face="normal" font="default" size="100%">Wandee Gritsanapan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Acetylcholinesterase inhibitory activity of didehydrostemofoline, stemofoline alkaloids and extracts from Stemona collinsiae Craib roots</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetylcholinesterase inhibition</style></keyword><keyword><style  face="normal" font="default" size="100%">Didehydrostemofoline</style></keyword><keyword><style  face="normal" font="default" size="100%">Stemofoline</style></keyword><keyword><style  face="normal" font="default" size="100%">Stemona collinsiae</style></keyword><keyword><style  face="normal" font="default" size="100%">Stemonaceae</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><volume><style face="normal" font="default" size="100%">5</style></volume><pages><style face="normal" font="default" size="100%">56-59</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Acetylcholinesterase (AChE) inhibition is one of insect toxicity mechanisms found in many plants. &lt;em&gt;Stemona collinsiae&lt;/em&gt; Craib, a traditional insecticidal plant, has been of interest for its AChE inhibitory activity.&lt;strong&gt; Methods:&lt;/strong&gt; Powdered roots of &lt;em&gt;S. collinsiae&lt;/em&gt; were successively extracted with hexane, dichloromethane, and methanol. The contents of major active insecticidal components, didehydrostemofoline and stemofoline alkaloids, were analyzed by HPLC. Anti-AChE activity was evaluated using Ellman&amp;rsquo;s colorimetric method and TLC-bioautography. &lt;strong&gt;Results:&lt;/strong&gt; The contents of didehydrostemofoline and stemofoline alkaloids in the hexane, dichloromethane, and methanol extracts were 3.59 and 0.18, 40.78 and 0.74, and 1.43 and 0.09% w/w, respectively. TLC fingerprints of each extract showed major spots of didehydrostemofoline and stemofoline of which TLC bioassays indicated active AChE inhibitory activity. IC&lt;sub&gt;50&lt;/sub&gt; values on anti-AChE activities of hexane, dichloromethane and methanol extracts were 126.72, 73.78, and &amp;gt;1000 mg/ml, respectively while those of didehydrostemofoline, stemofoline, and standard galanthamine were 131.3, 102.1, and 1.30 mM, respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; The AChE inhibitory activity of hexane, dichloromethane and methanol extracts from &lt;em&gt;S. collinsiae&lt;/em&gt; roots and their major alkaloids, didehydrostemofoline and stemofoline, were determined. The data support the traditional utilization of this plant as a natural insecticide.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; Acetylcholinesterase inhibition, Didehydrostemofoline, Stemofoline, &lt;em&gt;Stemona collinsiae&lt;/em&gt;, Stemonaceae.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;strong&gt;Sumet Kongkiatpaiboon&lt;sup&gt;a&lt;/sup&gt;, Piyanuch Rojsanga&lt;sup&gt;b&lt;/sup&gt;, Virote Pattarajinda&lt;sup&gt;c&lt;/sup&gt;, Wandee Gritsanapan&lt;sup&gt;a,*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;sup&gt;a&lt;/sup&gt;Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayutthaya Rd, Ratchathewi, Bangkok 10400, Thailand&lt;/p&gt;&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;sup&gt;b&lt;/sup&gt;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand&lt;/p&gt;&lt;p style=&quot;text-align: justify; &quot;&gt;&lt;sup&gt;c&lt;/sup&gt;Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand&lt;/p&gt;</style></auth-address></record></records></xml>