<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Desdiani Desdiani</style></author><author><style face="normal" font="default" size="100%">Iris Rengganis</style></author><author><style face="normal" font="default" size="100%">Samsuridjal Djauzi</style></author><author><style face="normal" font="default" size="100%">Agus Setiyono</style></author><author><style face="normal" font="default" size="100%">Mohamad Sadikin</style></author><author><style face="normal" font="default" size="100%">Sri Widia A Jusman</style></author><author><style face="normal" font="default" size="100%">Nuryati Chairani Siregar</style></author><author><style face="normal" font="default" size="100%">Suradi</style></author><author><style face="normal" font="default" size="100%">Putri C Eyanoer</style></author><author><style face="normal" font="default" size="100%">Fadilah Fadilah</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Assay and Study Interaction of Uncaria gambir (Hunter) Roxb. as Anti-fibrotic Activity Against A549 Cell Line</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Gambiriin compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">Inhibitor of p50 NF-κB</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Pulmonary fibrosis</style></keyword><keyword><style  face="normal" font="default" size="100%">TGF-β1 receptors</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1232-1240</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Aim: &lt;/strong&gt;The aim of this study is to finding inhibitor potential from several compounds in gambir plant by using&lt;em&gt; in vitro&lt;/em&gt; MTT assay and study interaction with molecular docking. The interaction of amino acids on the binding site with substances in the gambir plant was analyzed to determine its potential as a herbal-based therapy candidate for pulmonary fibrosis. &lt;strong&gt;Material and Methods:&lt;/strong&gt; Protein target using TGFβ1 and NF-κB and compounds from gambir plant ((+)-Catechin. Epigallocatechin gallate, (+)-Epicatechin, Gambiriin A1, Gambiriin A2, Gambiriin B1, Gambiriin B2, Gambiriin C, Procyanidin B1, Procyanidin B3). &lt;strong&gt;Result:&lt;/strong&gt; The results from docking analysis observed that compounds from gambir fruit contain anti-fibrotic activity which act by inhibiting DNA transcription of NF-κB and TGF-β1receptors. The compound Procyanidin B3, an essential amino acid, contains a hydrogen bond with the greatest NF-κB inhibitory activity on Gly214 and Lys337. Compounds from&lt;em&gt; Uncaria gambir &lt;/em&gt;(Hunter) Roxb. can be an inhibitor to TGFβ1, all the compounds are on the active site of TGFβ1, and use native ligand which is an inhibitor of TGFβ1 (Naphtyridine). The positive compound catechin has the highest inhibitory activity. Gambiriin B1 and Gambiriin A2 are the most identical compounds with similar affinity binding value. &lt;em&gt;Uncaria gambir&lt;/em&gt; (Hunter) Roxb. is already a proven antifibrotic which is further confirmed by (IC&lt;sub&gt;50&lt;/sub&gt;: 19,255 ± 1.08 μg/ml, p &amp;lt; 0.05) in A549 cell line. &lt;strong&gt;Conclusion: &lt;/strong&gt;The results demonstrated that Gambiriin have cytotoxic effects and was found potentially as anti-fibrotic by MTT assay and in silico evaluation.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1232</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Desdiani Desdiani&lt;sup&gt;1,&lt;/sup&gt;*, Iris Rengganis&lt;sup&gt;2&lt;/sup&gt;, Samsuridjal Djauzi&lt;sup&gt;2&lt;/sup&gt;, Agus Setiyono&lt;sup&gt;3&lt;/sup&gt;, Mohamad Sadikin&lt;sup&gt;4&lt;/sup&gt;, Sri Widia A. Jusman&lt;sup&gt;4&lt;/sup&gt;, Nuryati Chairani Siregar&lt;sup&gt;5&lt;/sup&gt;, Suradi&lt;sup&gt;6&lt;/sup&gt;, Putri C. Eyanoer&lt;sup&gt;7&lt;/sup&gt;, Fadilah Fadilah&lt;sup&gt;8,&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Medicine, Universitas Sultan Ageng Tirtayasa, Cilegon, Banten, Indonesia&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Veterinary Medicine IPB, Bogor, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department Anatomical Pathology, Faculty of medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Pulmonology and Respiratory Medicine, Faculty of medicine, Universitas Sebelas Maret, Surakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;7&lt;/sup&gt;Department of Community and Preventive Medicine, Universitas Sumatera Utara&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;8&lt;/sup&gt;Department of Medical Chemistry, Faculty of medicine, University of Indonesia; Bioinformatics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Jakarta, INDONESIA.&lt;/p&gt;
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