<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tiwuk Susantiningsih</style></author><author><style face="normal" font="default" size="100%">Ichwan Baihaki</style></author><author><style face="normal" font="default" size="100%">Maria Selvester Thadeaus</style></author><author><style face="normal" font="default" size="100%">Yuni Setyaningsih</style></author><author><style face="normal" font="default" size="100%">Mila Citrawati</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In-Silico Study of Bioactive Compounds from Moringa oleifera Fruit as Anti Premature Senescence Agents in Cardiac Cells: A Study on the p53 Protein</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">cellular senescence</style></keyword><keyword><style  face="normal" font="default" size="100%">in silico</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Moringa oleifera</style></keyword><keyword><style  face="normal" font="default" size="100%">p53 protein</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">497-505</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Cellular senescence, characterized by irreversible cell cycle arrest, contributes significantly to the pathogenesis of cardiovascular diseases through mechanisms involving oxidative stress and activation of p53-mediated signaling. &lt;em&gt;Moringa oleifera&lt;/em&gt;, widely recognized for its antioxidant properties, has demonstrated anti-aging effects; however, the specific bioactive compounds within its fruit and their mechanisms of action remain poorly understood. &lt;strong&gt;Objective: &lt;/strong&gt;This study aimed to investigate the potential of &lt;em&gt;M. oleifera &lt;/em&gt;fruit-derived compounds as anti-premature senescence agents targeting the p53 protein using in-silico molecular docking approaches. Methods: Bioactive compounds from &lt;em&gt;M. oleifera&lt;/em&gt; fruit were screened via molecular docking against the human p53 protein, with Nutlin-3 used as a positive control. Binding affinities, hydrogen bonding, and hydrophobic interactions were analyzed to determine ligand– receptor interactions. &lt;strong&gt;Results: &lt;/strong&gt;Niacin and oxalic acid exhibited stronger binding affinities (–5.90 and –6.00 kcal/mol, respectively) compared to Nutlin-3 (–5.64 kcal/mol). Niacin formed stable hydrogen bonds and hydrophobic interactions with key residues within the p53 active site, suggesting a capacity to modulate p53 activity. Oxalic acid demonstrated the highest binding affinity but lacked hydrogen bonding, indicating potential instability despite strong interaction. These findings support previous studies highlighting &lt;em&gt;M. oleifera's &lt;/em&gt;role in ROS suppression and p53 modulation, pointing to its therapeutic relevance in mitigating cellular aging. &lt;strong&gt;Conclusion: &lt;/strong&gt;Niacin and oxalic acid from &lt;em&gt;M. oleifera&lt;/em&gt; exhibit promising binding characteristics as modulators of the p53 pathway. Their anti-senescence potential warrants further validation through molecular dynamics simulations and biological assays. This study supports the development of natural compound-based therapeutics for age-related cardiac degeneration.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">497</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Tiwuk Susantiningsih&lt;sup&gt;1,2&lt;/sup&gt;, Ichwan Baihaki&lt;sup&gt;1*&lt;/sup&gt;, Maria Selvester Thadeaus&lt;sup&gt;1,2&lt;/sup&gt;, Yuni Setyaningsih&lt;sup&gt;1,2&lt;/sup&gt;, Mila Citrawati&lt;sup&gt;1,2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, Jakarta, 12450, INDONESIA&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Research Centre for Moringa Oleifera, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, Jakarta, 12450, INDONESIA&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mila Citrawati</style></author><author><style face="normal" font="default" size="100%">Assyafiya Salwa</style></author><author><style face="normal" font="default" size="100%">Yuni Setyaningsih</style></author><author><style face="normal" font="default" size="100%">Cut Fauziah</style></author><author><style face="normal" font="default" size="100%">Tiwuk Susantiningsih</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Moringa oleifera Fruit Secondary Metabolites Role in Sarcopenic Obesity via Fat Mass and Obesity-Associated Protein: An In Silico Analysis</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">and riboflavin</style></keyword><keyword><style  face="normal" font="default" size="100%">FTO protein</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Moringa oleifera fruit</style></keyword><keyword><style  face="normal" font="default" size="100%">sarcopenic obesity (SO)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">450-457</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Sarcopenic obesity (SO) refers to the coexistence of sarcopenia and obesity, pathogenic interaction between loss of skeletal muscle and function and fat-mass accumulation. Fat mass and obesityassociated (FTO) protein is one of the proteins that involved in pathophysiology of SO. &lt;em&gt;Moringa oleifera&lt;/em&gt; is one of potential drug candidates for degenerative diseases due to its various bioactive metabolites from most parts of this plant. &lt;strong&gt;Objective:&lt;/strong&gt; An&lt;em&gt; in silico&lt;/em&gt; study, employing computational methods to simulate molecular interactions through molecular docking, aims to investigate the potential of &lt;em&gt;Moringa oleifera&lt;/em&gt; fruit secondary metabolites to interact with FTO protein. &lt;strong&gt;Methodology:&lt;/strong&gt; This study was carried out the molecular docking analysis of &lt;em&gt;Moringa oleifera&lt;/em&gt; fruit secondary metabolites that was retrieved from database and have been screened for drug-likeness and toxicity for FTO protein inhibitor candidates. Molecular docking was using Pyrx v0.8, AutoDock 4.2.6 by AutoDockTools 1.5.7, and BIOVIA Discovery studio client 2025 as visualization tools. &lt;strong&gt;Results:&lt;/strong&gt; This study showed 9 bioactive compounds from &lt;em&gt;Moringa oleifera&lt;/em&gt; fruit is bioavailable and safe for oral drugs according to Lipinski Rule of 5 (RO5) and Oral Rat Acute Toxicity (LD50). Molecular docking results showed riboflavin is the most potential compound as FTO protein inhibitor as its strongest affinity and interaction in active site compared to FTO protein native ligands 3-methylthymidine (DT). &lt;strong&gt;Conclusion: &lt;/strong&gt;Therefore, &lt;em&gt;Moringa oleifera &lt;/em&gt;fruit is potential for SO therapy candidates through regulation of FTO protein activity.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">450</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Mila Citrawati&lt;sup&gt;1,2&lt;/sup&gt;, Assyafiya Salwa&lt;sup&gt;1*&lt;/sup&gt;, Yuni Setyaningsih&lt;sup&gt;1,2&lt;/sup&gt;, Cut Fauziah&lt;sup&gt;1&lt;/sup&gt;, Tiwuk Susantiningsih&lt;sup&gt;1,2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, Jakarta, 12450, INDONESIA&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Research Centre for Moringa Oleifera, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, Jakarta, 12450, INDONESIA&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Feda Makkiyah</style></author><author><style face="normal" font="default" size="100%">Eldiza Puji Rahmi</style></author><author><style face="normal" font="default" size="100%">Yuni Setyaningsih</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemical Test and Acute Safety Evaluation of Oral Purple Leaves (Graptophyllum Pictum L. Griff) Extract in Rats</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acute</style></keyword><keyword><style  face="normal" font="default" size="100%">purple leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Safety</style></keyword><keyword><style  face="normal" font="default" size="100%">Toxicity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">649-654</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Purple leaves (&lt;em&gt;Graptophyllum pictum&lt;/em&gt; (L) Griff) is a native plant from Indonesia (Polynesia) which is empirically common used to treat hemorrhoids, diabetes, and many other diseases. Despite its massive development globally, there are few studies have written about the toxicity of this herbal medicine. &lt;strong&gt;Aim: &lt;/strong&gt;The aim of this study is to describe the acute toxicity of this herbal medicine, as the basic ground of the further safe development of this medicine. &lt;strong&gt;Method&lt;/strong&gt;: Each purple leaf dry powder (2 kg) was extracted with 15 L of 70% ethanol and 15 L of hexane by maceration method for 3 days at room temperature and then filtered to obtain macerate liquid. The study was conducted on 36 male 6-8 weeks, Sprague–Dawley (SD) rats. The result was presented as the mean value ± standard deviation (SD). Data were evaluated for homogeneity using Saphiro Wilk. The comparisons between pre dan post treatment body weight were measured by paired student t test and hematological measurements were analyzed by ANOVA. P value less than 0.05 was considered significant. &lt;strong&gt;Results&lt;/strong&gt;: Acute toxicity of ethanol and hexane extract were assessed following a single dose administered by gavage at a dose of 50, 2000 and 3000 g/kg bw. The mean body weight of rats increased from 150 ± 2.22 g to 161 ± 6.68 g during the 14 days. The difference of body weight between before and at 14 days amongs groups were significantly different with P value &amp;lt;0.01. &lt;strong&gt;Conclusion:&lt;/strong&gt; The present study showed that hexane extract contains more valuable components for medical treatment purposes. The acute toxicity on 50, 2000,3000 kg/BW oral ethanol and hexane extract of &lt;em&gt;Graptophyllum pictum&lt;/em&gt; showed no significant influence on hematological blood parameters of rats. It is safe to administered orally ethanol or hexane extract of G. pictum below 3000mg/kgBW rats.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">649</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Feda Makkiyah*, Eldiza Puji Rahmi, Yuni Setyaningsih&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Faculty of Medicine, UPN Veteran Jakarta, Indonesia.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Feda Makkiyah</style></author><author><style face="normal" font="default" size="100%">Eldiza Puji Rahmi</style></author><author><style face="normal" font="default" size="100%">Rika Revina</style></author><author><style face="normal" font="default" size="100%">Tiwuk Susantiningsih</style></author><author><style face="normal" font="default" size="100%">Yuni Setyaningsih</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Graptophyllum pictum (L.) Griff. (Syn: Justicia picta Linn.) and its Effectiveness: A Well-Known Indonesian Plant</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Activities</style></keyword><keyword><style  face="normal" font="default" size="100%">G pictum</style></keyword><keyword><style  face="normal" font="default" size="100%">Indonesia</style></keyword><keyword><style  face="normal" font="default" size="100%">Pharmacological</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">835-838</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;em&gt;Graptophyllum pictum &lt;/em&gt;(L.) Griff., a shrubby stove plant, has been used traditionally to treat various diseases. This review summarizes the up-to-date data about its phytochemistry and pharmacological properties and provides a perspective and direction for future research. The phytochemical analysis of &lt;em&gt;G. pictum&lt;/em&gt; revealed the presence of flavonoids, steroids, glycoside, tannins, saponin, chlorophyll, nontoxic alkaloid, and anthocyanin. Meanwhile, the pharmacological analysis revealed that this plant possessed various activities such as antimicrobial, immunomodulatory, antioxidant, anti-inflammation, anti-analgetic, woundhealing, anti-hemorrhoid, anti-diabetic, and estrogenic effects. However, most of its pharmacological activities were based on the crude extracts and the bioactive compounds responsible for the bioactivities have not been well identified. A further detailed investigation in phytochemistry to identify the bioactive compounds and to investigate the mechanism action of its bioactivities is required. Moreover, a toxicology study is also needed to develop a standardized formulation of &lt;em&gt;G. pictum&lt;/em&gt; preparation and to fulfill the requirements of modern phytomedicine.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Review Article</style></work-type><section><style face="normal" font="default" size="100%">835</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Feda Makkiyah&lt;sup&gt;1,&lt;/sup&gt;*, Eldiza Puji Rahmi&lt;sup&gt;2&lt;/sup&gt;, Rika Revina2, Tiwuk Susantiningsih&lt;sup&gt;1&lt;/sup&gt;, Yuni Setyaningsih&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacy, Universitas Pembangunan Nasional Veteran Jakarta, INDONESIA.&lt;/p&gt;
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