<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ashwani Kumar</style></author><author><style face="normal" font="default" size="100%">Prikshat Kumar Angra</style></author><author><style face="normal" font="default" size="100%">Suresh Chandra Akula</style></author><author><style face="normal" font="default" size="100%">Pritpal Singh</style></author><author><style face="normal" font="default" size="100%">Anuj Sharma</style></author><author><style face="normal" font="default" size="100%">Anup Sharma</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Efficacy of Standardized Withania Somnifera as Neuro Agent in Tension-Type Headache: A Comparative Trial of Data in Transit</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biomarker Integrity</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemical Standardization</style></keyword><keyword><style  face="normal" font="default" size="100%">Sustainable Research Framework</style></keyword><keyword><style  face="normal" font="default" size="100%">Tension-Type Headache</style></keyword><keyword><style  face="normal" font="default" size="100%">Withania somnifera</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">378-385</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; To evaluate the role of withania somnifera as a neuro-psychiatric agent in the treatment of Tension-Type Headache (TTH), there is a need to substantiate the therapy using solid therapeutic evidence. Although using traditional medicine has a great potential, it is difficult to demonstrate the efficacy of a particular phytochemical composition regarding the frequency of and physiological stress markers of the chronic Tension-Type Headache in standardized research. &lt;strong&gt;Objective: &lt;/strong&gt;The purpose of the study is to assess the therapeutic efficacy of a standardized Withania somnifera extract in reducing the frequency of and physiological stress markers of the chronic Tension-Type Headache. Methods: We used the study as a double-blind and randomized controlled trial (RCT). The subjects were randomly selected to be given a standardized extract of the root of W. somnifera, that has been titrated to contain 5 percent withanolides, or a placebo dose of 60 days. The paper ensured a rigorous &quot;molecular chain of custody&quot; with a batch of withanolides linked in clinical outcome reported where the mean frequency of headaches in the treatment group reduced by 42% (p [Less than] 0.005). &lt;strong&gt;Results:&lt;/strong&gt; Clinical outcome showed that there was a significant decline in the frequency of headaches in the treatment group by 42% (p [Less than] 0.005). Moreover, the Ashwagandha group was showing a significant drop in salivary cortisol levels, which means that there was a decrease in the level of physiological stress response to chronic TTH. &lt;strong&gt;Conclusion:&lt;/strong&gt; Standardized natural products should be integrated into clinical trials as it is the most beneficial to the development of sustainable pharmacognosy. This experiment can offer an unambiguous evidence base, a clear and reproducible basis of evidence regarding the use of Ashwagandha in the current neurological practice by showing the clear efficacy in reducing the frequency of Tension-Type Headaches and biological stress markers.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">378</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ashwani Kumar&lt;sup&gt;2*&lt;/sup&gt;, Prikshat Kumar Angra&lt;sup&gt;2&lt;/sup&gt;, Suresh Chandra Akula&lt;sup&gt;1&lt;/sup&gt;, Pritpal Singh&lt;sup&gt;1&lt;/sup&gt;, Anuj Sharma&lt;sup&gt;2&lt;/sup&gt;, Anup Sharma&lt;sup&gt;1&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Mittal School of Business, Lovely Professional University, Phagwara, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;School of Computer Applications, Lovely Professional University, Phagwara, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jitender Singh</style></author><author><style face="normal" font="default" size="100%">Ashwani Kumar</style></author><author><style face="normal" font="default" size="100%">Anupam Sharma</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bioactivity Guided Fractionation of Ethanol Extract of Caesalpinia digyna Rottler Roots</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antianxiety</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioactivity-guided fractionation</style></keyword><keyword><style  face="normal" font="default" size="100%">Caesalpinia digyna</style></keyword><keyword><style  face="normal" font="default" size="100%">Elevated plus-maze.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">165-167</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Authors have reported earlier that ethanol extract of &lt;em&gt;Caesalpinia digyna&lt;/em&gt; Rottler roots exhibits significant antianxiety activity at 400 mg/kg, po, in mice using elevated plus-maze (EPM).&lt;strong&gt; Objective&lt;/strong&gt;: Aim of the study was to isolate antianxiety principle(s) from ethanol extract of &lt;em&gt;C. digyna&lt;/em&gt; roots following bioactivity guided fractionation approach. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Bioactive ethanol extract was partitioned with ethyl acetate to get ethyl acetate soluble (EASF) and ethyl acetate insoluble (EAIF) fractions. A compound (CD&lt;sub&gt;1&lt;/sub&gt;) precipitated from EASF. The two fractions and CD&lt;sub&gt;1&lt;/sub&gt; were evaluated for antianxiety activity in mice. Column chromatography of EASF yielded 5 fractions (F&lt;sub&gt;1&lt;/sub&gt;-F&lt;sub&gt;5&lt;/sub&gt;), all of which were evaluated for antianxiety activity using EPM.&lt;strong&gt; Results:&lt;/strong&gt; Present study revealed that EASF (80 mg/kg) and CD&lt;sub&gt;1&lt;/sub&gt; (40 mg/kg) exhibited significant antianxiety activity, while EAIF does not. Among the five fractions, only F4 (40 mg/kg, po), exhibited significant antianxiety activity, which was statistically comparable to that of diazepam (2 mg/kg). &lt;strong&gt;Conclusion: &lt;/strong&gt;Present investigation reveals that EASF obtained by partitioning of ethanol extract of &lt;em&gt;C. digyna &lt;/em&gt;roots with ethyl acetate, and a compound CD&lt;sub&gt;1&lt;/sub&gt;, isolated from EASF, exhibit significant antianxiety activity. Among 5 fractions (F&lt;sub&gt;1&lt;/sub&gt;-F&lt;sub&gt;5&lt;/sub&gt;) obtained from column chromatography of EASF, only F4 exhibited significant antianxiety activity. F4 is being processed further to isolate the anxiolytic constituent(s), and CD&lt;sub&gt;1&lt;/sub&gt; is being characterized.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">165</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;&lt;strong&gt;Jitender Singh, Ashwani Kumar*, Anupam Sharma&lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align:justify&quot;&gt;Department of Pharmacognosy, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160014, INDIA.&lt;/p&gt;

&lt;p style=&quot;text-align:justify&quot;&gt;&amp;nbsp;&lt;/p&gt;
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