<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Silvia W Lestari</style></author><author><style face="normal" font="default" size="100%">Khairunnisa F Ilato</style></author><author><style face="normal" font="default" size="100%">M. Iqbal A Pratama</style></author><author><style face="normal" font="default" size="100%">Husna Qonitah</style></author><author><style face="normal" font="default" size="100%">Gito Wasian</style></author><author><style face="normal" font="default" size="100%">Amandanu Bramantya</style></author><author><style face="normal" font="default" size="100%">Firda A Husna</style></author><author><style face="normal" font="default" size="100%">Ria Margiana</style></author><author><style face="normal" font="default" size="100%">Gita Pratama</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Does Soybean (Glycine max) Supplementation Increase Oocyte Viability, Decrease Oocyte Apoptosis and Improve Embryo Development Quality? An Experimental Study</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">133-140</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; One of the important factors in Assisted Reproductive Technology (ART) is embryo quality that depends on oocyte quality. Maternal nutrition in form of soybean supplementation is thought to have benefits in oocyte quality. To determine the effect soybean supplementation to the embryo quality from oocyte side. &lt;strong&gt;Methods: &lt;/strong&gt;This experimental study involved female mice from Swiss strain aged 6 weeks that were divided into two groups, group with soybean (soybean group/SG) and group without soybeans (pellet group/PG). Follicles were collected and denuded to get oocytes. The oocytes were stained with MitoTracker for assessing the mitochondrial membrane potential and TUNEL for assessing the apoptotic level. Colour intensity was assessed using a confocal microscope and determined using ImageJ software. Sperms were extracted surgically from the epididymis-vas deferens and performed preparation prior to intra cytoplasmic sperm injection (ICSI) procedure. Then, the embryos were cultured and observed for the quality. &lt;strong&gt;Result&lt;/strong&gt;: In TUNEL test, the average colour intensity of the SG was lower compared to the PG, significantly (p=0.03). While in the Mito Tracker test, the average colour intensity for the SG was higher compared to PG, not significantly (p=1.08). In addition, the embryo development on Day-1 and Day-3 showed more good embryo quality of SG compared to PG, significantly (p=0.03). &lt;strong&gt;Conclusion&lt;/strong&gt;: Soybean supplementation improved embryo quality at the cleavage stage by decreasing apoptosis of the oocytes rather than increasing the viability the oocytes.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Key words&lt;/strong&gt;: Oocyte, Soybean, Apoptosis, Embryo development, Embryo quality.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">133</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Silvia W Lestari&lt;sup&gt;1,*&lt;/sup&gt;, Khairunnisa F Ilato&lt;sup&gt;2&lt;/sup&gt;, M. Iqbal A Pratama&lt;sup&gt;2&lt;/sup&gt;, Husna Qonitah&lt;sup&gt;3&lt;/sup&gt;, Gito Wasian&lt;sup&gt;1&lt;/sup&gt;, Amandanu Bramantya&lt;sup&gt;2&lt;/sup&gt;, Firda A Husna&lt;sup&gt;4&lt;/sup&gt;, Ria Margiana&lt;sup&gt;5&lt;/sup&gt;, Gita Pratama&lt;sup&gt;6&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Undergraduate Study of Medicine of Faculty Medicine, Universitas INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Undergraduate Study of Biology of Faculty Mathematics and Natural Science, Universitas INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Master’s programme in biomedical medicine of Faculty Medicine, Universitas INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Obstetry &amp;amp; Gynecology, Faculty of Medicine, Universitas Indonesia – Ciptomangunkusumo Hospital, Jakarta, INDONESIA.&lt;/p&gt;
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