<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tiwuk Susantiningsih</style></author><author><style face="normal" font="default" size="100%">Fadilah Fadilah</style></author><author><style face="normal" font="default" size="100%">Ani Retno Prijanti</style></author><author><style face="normal" font="default" size="100%">Novi Silvia Hardiany</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular Insights into Spirulina platensis Compounds Targeting TNFa and P21 in Delaying Cellular Senescence Mechanisms: An In Silico Approach</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">in silico</style></keyword><keyword><style  face="normal" font="default" size="100%">p21 expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Spirulina platensis</style></keyword><keyword><style  face="normal" font="default" size="100%">TNFa-induced cellular senescence</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">809-815</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;!-- x-tinymce/html --&gt;Spirulina platensis with bioactive compounds such as Phycocyanin, β-carotene, Genistein and α-glucan, have been shown to have anti-inflammatory properties. Our present study investigation utilizes an in silico methodology to examine the molecular interactions between Spirulina platensis chemicals compound with TNFa and p21. Utilizing computational techniques molecular docking, our goal is to clarify the possible pathways by which Spirulina platensis chemicals could affect these important regulators and postpone cellular senescence. Our research may shed important light on the creation of Spirulina platensis-based therapies for ageing and age-related illnesses. Our results imply that Spirulina platensis may contribute to overall cellular health and the mitigation of cellular senescence. Phycocyanin has the most negative ΔG value is -15.0 kcal/mol. Genistein has the lowest Ki value, namely 7.299 μM. The ΔG and Ki values of Genistein were lower than Quercetin. The potential chemical interactions between substances generated from Spirulina platensis and senescence pathways, including those involving TNFa and p21, are highly intriguing for the development of innovative therapeutic approaches targeted at ameliorating cellular senescence dysfunction associated with aging.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">809</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;!-- x-tinymce/html --&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Tiwuk Susantiningsih&lt;sup&gt;1,2&lt;/sup&gt;, Fadilah Fadilah&lt;sup&gt;3*&lt;/sup&gt;, Ani Retno Prijanti&lt;sup&gt;4&lt;/sup&gt;, Novi Silvia Hardiany&lt;sup&gt;5&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biochemistry Faculty of Medicine, UPN Veteran Jakarta, Jakarta, 12450, INDONESIA.&lt;sup&gt; &lt;/sup&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, Jakarta, 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Magister Program of Biomedical Science, Faculty of Medicine, University of Indonesia, Jakarta, 10430, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rangki Astiani</style></author><author><style face="normal" font="default" size="100%">Mohamad Sadikin</style></author><author><style face="normal" font="default" size="100%">Aprilita Rinayanti</style></author><author><style face="normal" font="default" size="100%">Wawaimuli Arozal</style></author><author><style face="normal" font="default" size="100%">Ani Retno Prijanti</style></author><author><style face="normal" font="default" size="100%">Fadilah Fadilah</style></author><author><style face="normal" font="default" size="100%">Firdayani Firdayani</style></author><author><style face="normal" font="default" size="100%">Piter Piter</style></author><author><style face="normal" font="default" size="100%">Guntoro Halim</style></author><author><style face="normal" font="default" size="100%">Franciscus D. Suyatna</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Study of Triterpene Saponin Compounds from Centella asitica as Renin Inhibitor with Pharmacophore Modeling, Molecular Docking and In-vitro Evaluation</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antihypertensive.</style></keyword><keyword><style  face="normal" font="default" size="100%">Asiaticoside</style></keyword><keyword><style  face="normal" font="default" size="100%">Centella asiatica</style></keyword><keyword><style  face="normal" font="default" size="100%">In-vitro</style></keyword><keyword><style  face="normal" font="default" size="100%">Madecasoside</style></keyword><keyword><style  face="normal" font="default" size="100%">Renin inhibitor</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">57-63</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Hypertension is a silent killer that causes kidney, heart, and stroke damage if not handled properly. In Indonesia, the prevalence of the population with high blood pressure is 34.11% with women 36.85% higher than men 31.34%, this shows a fairly high value so that special attention is needed on hypertension therapy. It is known that currently there are 6 types of pharmacological therapy for hypertension and one of the newest is the renin inhibitor class (Aliskiren). Indonesia has diverse natural wealth in the form of flora and fauna, with a wealth of more than 30,000 types of medicinal plants with 9500 potential herbal medicines that have not been utilized optimally, with the largest exporter of herbal medicines in the world. &lt;em&gt;Centella asiatica&lt;/em&gt; plants containing triterpenoid saponins have high renin inhibitor activity, namely the content of Asiaticoside and Madecasoside. The research method was carried out &lt;em&gt;in silico&lt;/em&gt; using molecular simulation and &lt;em&gt;in vitro &lt;/em&gt;with fluorometry (328/552 nm) to test the activity of asiaticoside and madecasoside compounds as well as a mixture of asiaticoside and madecasoside in &lt;em&gt;Centella asiatica&lt;/em&gt; plants. This is supported by the docking outcome. The docking results show that madecososide compounds have a gibbs energy close to the positive control aleskiren (-8.356 kcal/mol) and aleskiren (-9.44 kcal/mol). The experiment results showed that the triterpenoid saponin compound (madecassoside) contained an IC value of 0.71, at a concentration of 5 μg/μl, and absorbance of 1.35 A in the first minute. The strongest renin inhibition was Madecasoside compound with a concentration of 5 μg/μl with an average value of fluorescent adsorption and an average percent inhibition of 135% with the best renin inhibition at Madecasoside 5 ug/ul the first minute with absorbance values 1.19 A. Finally, the &lt;em&gt;in silico&lt;/em&gt; result corresponded to the &lt;em&gt;in vitro&lt;/em&gt; experiment. &lt;em&gt;Centella asiatica&lt;/em&gt; plants have renin inhibitor activity as antihypertensive, especially in secondary metabolites of triterpene saponins with pure madecasoside compounds compared with aliskiren as a renin inhibitor. So that the compound madecasoside has renin inhibitor activity as an antihypertensive.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article </style></work-type><section><style face="normal" font="default" size="100%">57</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Rangki Astiani&lt;sup&gt;1,3,*&lt;/sup&gt;, Mohamad Sadikin&lt;sup&gt;1,2&lt;/sup&gt;, Aprilita Rinayanti&lt;sup&gt;4&lt;/sup&gt;, Wawaimuli Arozal&lt;sup&gt;1,5&lt;/sup&gt;, Ani Retno Prijanti&lt;sup&gt;1,2&lt;/sup&gt;, Fadilah Fadilah&lt;sup&gt;1,6,7&lt;/sup&gt;, Firdayani Firdayani&lt;sup&gt;8&lt;/sup&gt;, Piter Piter&lt;sup&gt;3&lt;/sup&gt;, Guntoro Halim&lt;sup&gt;3&lt;/sup&gt;, Franciscus D. Suyatna&lt;sup&gt;1,5&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program in Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia 10320, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biology Molecular and Biochemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10320, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Pharmacy, Universitas 17 Agustus 1945 Jakarta, Indonesia 14350, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacy Faculty of Health Sciences, Universitas Esa Unggul, Jakarta 11510, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;7&lt;/sup&gt;Bioinformatics Core Facilities - IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;8&lt;/sup&gt;Technology Assesment and Application Agency (BPPT), Indonesian Science and Technology Research Center, INDONESIA.&lt;/p&gt;
</style></auth-address></record></records></xml>