<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Roohollah Sobhani</style></author><author><style face="normal" font="default" size="100%">Amarta Kumar Pal</style></author><author><style face="normal" font="default" size="100%">Atanu Bhattacharjee</style></author><author><style face="normal" font="default" size="100%">Sivaprasad Mitra</style></author><author><style face="normal" font="default" size="100%">Kripamoy Aguan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Screening Indigenous Medicinal Plants of Northeast India for Their Anti-Alzheimer’s Properties</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AChE-inhibition</style></keyword><keyword><style  face="normal" font="default" size="100%">Alzheimer’s</style></keyword><keyword><style  face="normal" font="default" size="100%">Ellman assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Kinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicinal plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2016</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">46-54</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Alzheimer&amp;rsquo;s disease (AD) is a progressive neurologic disease of the brain that affects intellectual abilities, reasoning and memory. Acetylcholine (ACh) is involved in the maintenance of cognitive process. Pathologically, ACh production is compromised in the brains of AD affected people. Presence of acetylcholinesterase (AChE) in the synaptic cleft, which hydrolyzes ACh, further decreases the ACh-levels, and thereby, additionally compromises cognition. The tribal people of North East India have been using indigenous plants as traditional medicine for brain disorders. We assayed whether the plants used in the traditional tribal knowledge for the treatment of brain disorders might contain better AChE-inhibitors. &lt;strong&gt;Methods: &lt;/strong&gt;We collected 10 traditional medicinal plants from Northeast India. A total of 39 plant extracts were prepared using three solvent systems. The Acetylcholinesterase (AChE) activity was measured with Ellman method. The experiment was done in triplicate for each level of inhibitor. The activity was measured at 412 nm wavelength using Plate Reader. The standard student t-test was used to show significant difference in IC&lt;sub&gt;50&lt;/sub&gt; values between extracts. &lt;strong&gt;Results:&lt;/strong&gt; The result are reported based on Km, Vmax, IC&lt;sub&gt;50&lt;/sub&gt; (&lt;em&gt;&amp;mu;&lt;/em&gt;g/&lt;em&gt;&amp;mu;&lt;/em&gt;l), percentage inhibition and inhibition pattern. Two extracts had competitive inhibition, 11 extracts had mixed inhibition, 2 extracts had non-competitive inhibition, 11 extracts had uncompetitive inhibition and 4 extracts did not provide any proper pattern. The IC&lt;sub&gt;50&lt;/sub&gt; for these plant extracts were at the range of 0.51-12.4 &lt;em&gt;&amp;mu;&lt;/em&gt;g/&lt;em&gt;&amp;mu;&lt;/em&gt;l. Notably, Cinnamomum camphora (leaf: chloroform), Litsea glutinosa (stem; chloroform), and Litsea glutinosa (stem; methanol) showed IC50 values of 0.51, 0.53 &amp;amp; 0.81 &lt;em&gt;&amp;mu;&lt;/em&gt;g/&lt;em&gt;&amp;mu;&lt;/em&gt;l, respectively.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">46</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Roohollah Sobhani&lt;sup&gt;1&lt;/sup&gt;, Amarta Kumar Pal&lt;sup&gt;2&lt;/sup&gt;, Atanu Bhattacharjee&lt;sup&gt;3&lt;/sup&gt;, Sivaprasad Mitra&lt;sup&gt;4&lt;/sup&gt;, Kripamoy Aguan&lt;sup&gt;5&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong-793022, INDIA.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Chemistry, North Eastern Hill University, Shillong-793022, INDIA.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong-793022, INDIA.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Chemistry, North Eastern Hill University, Shillong-793022, INDIA. &lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong-793022, INDIA.&lt;/p&gt;
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