<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Veshalini K</style></author><author><style face="normal" font="default" size="100%">Daryl Jesus Arapoc</style></author><author><style face="normal" font="default" size="100%">Zainah Adam</style></author><author><style face="normal" font="default" size="100%">Rosniza Razali</style></author><author><style face="normal" font="default" size="100%">Noor Azuin Suliman</style></author><author><style face="normal" font="default" size="100%">Noor Azlina Abu Bakar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemical Screening, In vitro Antioxidant Activities and Zebrafish Embryotoxicity of Abelmoschus esculentus Extracts</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Abelmoschus esculentus</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant Capacity</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemicals</style></keyword><keyword><style  face="normal" font="default" size="100%">Zebrafish embryotoxicity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">690-701</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;em&gt;Abelmoschus esculentus&lt;/em&gt; (L.) Moench (&lt;em&gt;A. esculentus&lt;/em&gt;) commonly known as okra is being used as a medicinal plant traditionally, due to its phytochemical content that exhibits significant biological activities. &lt;strong&gt;Objective: &lt;/strong&gt;The present study was undertaken to determine phytochemicals, antioxidant activity and embryotoxic effects of hexane extract (HE), chloroform extract (CE), methanol extract (ME), and aqueous extract (AE) of &lt;em&gt;A. esculentus &lt;/em&gt;cultivated in Malaysia. &lt;strong&gt;Materials and Methods&lt;/strong&gt;:&lt;em&gt; A. esculentus&lt;/em&gt; extracts were screened for the phytochemicals while&lt;em&gt; in vitro &lt;/em&gt;antioxidant activities were evaluated by performing 1,1-diphenyl-2-picrylhidrazyl (DPPH) assay and reducing power assay. Meanwhile, the embryotoxicity were accessed by exposing zebrafish embryos to the extracts and developmental endpoint recorded with median lethal concentration (LC&lt;sub&gt;50&lt;/sub&gt;)&lt;strong&gt;. Results: &lt;/strong&gt;The phytochemical screening showed the presence of flavonoids, tannins, phenols, saponins, anthraquinones, alkaloids and reducing sugars. The highest phenolic content was obtained in the CE at 143.85 μg (GAE)/mg extract. Whereas the HE depicted the highest flavonoid content at 63.76 μg (QE)/mg extract. At 1000 μg/mL, ME showed the highest free radical scavenging activity at 28.86 % and reducing power at 0.271, in both DPPH and reducing power assay, respectively. While the CE presented a significant embryotoxic effect on zebrafish with the LC&lt;sub&gt;50&lt;/sub&gt; at 236.07 μg/mL. The morphological malformation of embryos, scoliosis and pericardial oedema were observed at 500 μg/mL of HE and ME treatment. &lt;strong&gt;Conclusion:&lt;/strong&gt; As a result, with various ranges of phytochemical compounds, antioxidant capacities and lower toxic effects of&lt;em&gt; A. esculentus &lt;/em&gt;extracts may be efficient in reducing the inflammation precursors that drive chronic inflammatory illnesses.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><accession-num><style face="normal" font="default" size="100%">28</style></accession-num><section><style face="normal" font="default" size="100%">690</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Veshalini K&lt;sup&gt;1,2&lt;/sup&gt;, Daryl Jesus Arapoc&lt;sup&gt;2&lt;/sup&gt;, Zainah Adam2, Rosniza Razali&lt;sup&gt;2&lt;/sup&gt;, Noor Azuin Suliman&lt;sup&gt;1,*&lt;/sup&gt;, Noor Azlina Abu Bakar&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, 20400 Kuala Terengganu, Terengganu, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Medical Technology Group, Malaysian Nuclear agency, 43000 Kajang, Selangor, MALAYSIA&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">BiYun Gu</style></author><author><style face="normal" font="default" size="100%">Joseph Shalom</style></author><author><style face="normal" font="default" size="100%">Ian E. Cock</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anti-Proliferative Properties of Terminalia sericea Burch. Ex Dc Leaf Extracts Against Caco2 and HeLa Cancer Cell Lines</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant Capacity</style></keyword><keyword><style  face="normal" font="default" size="100%">Antiproliferative Activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Combretaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">DPPH</style></keyword><keyword><style  face="normal" font="default" size="100%">Silver Cluster Leaf</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/499</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">408-415</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Terminalia&lt;/em&gt; spp. are characterised by their high levels of antioxidant phytochemicals and several species have anticancer activity. This study examines the anti-proliferative activity of &lt;em&gt;T. sericea&lt;/em&gt; leaf extracts against Caco2 and HeLa carcinoma cell proliferation. &lt;strong&gt;Methods:&lt;/strong&gt; Solvent extracts were prepared from &lt;em&gt;T. sericea&lt;/em&gt; leaves and their antioxidant capacities were determined by the DPPH free radical scavenging assay. Anti-proliferative activities against Caco2 and HeLa cancer cells were determined by an MTS based cell proliferation assay. Toxicity was determined using the &lt;em&gt;Artemia franciscana&lt;/em&gt; nauplii bioassay. &lt;strong&gt;Results:&lt;/strong&gt; The methanolic and aqueous &lt;em&gt;T. sericea&lt;/em&gt; leaf extracts displayed high antioxidant capacities (equivalent to 150 and 340 mg of ascorbic acid per gram of plant material extracted respectively). In contrast, the ethyl acetate, chloroform and hexane extracts had relatively low antioxidant contents (&amp;le;5 mg of ascorbic acid equivalents per gram of plant material extracted). The antioxidant contents of the &lt;em&gt;T. sericea&lt;/em&gt; leaf extracts correlated with the ability of the extracts to inhibit proliferation of Caco2 and HeLa cancer cell lines. The high antioxidant methanolic and aqueous extracts were potent inhibitors of cell proliferation, with IC&lt;sub&gt;50&lt;/sub&gt; values 120-1400 &amp;mu;g/mL. The aqueous &lt;em&gt;T. sericea&lt;/em&gt; leaf extract was particularly effective, with IC&lt;sub&gt;50&lt;/sub&gt; values of 528 and 120 &amp;mu;g/mL against Caco2 and HeLa cells respectively. The methanolic extract also displayed good, albeit substantially less potent, antiproliferative activity against HeLa cells, with an IC&lt;sub&gt;50&lt;/sub&gt; of 1358 &amp;mu;g/mL. In contrast, the lower antioxidant content extracts generally did not inhibit cancer cell proliferation. Cell imaging studies detected morphological features consistent with apoptosis in Caco2 cells exposed to sub-lethal concentrations of the methanolic and aqueous T. sericea leaf extracts, indicating that these extracts are functioning by cytotoxic mechanisms. The aqueous &lt;em&gt;T. sericea&lt;/em&gt; leaf extract displayed low to moderate toxicity in the &lt;em&gt;Artemia franciscana&lt;/em&gt; bioassay, with an LC&lt;sub&gt;50&lt;/sub&gt; value of 737 &amp;mu;g/mL. All other extracts were nontoxic. &lt;strong&gt;Conclusion:&lt;/strong&gt; The antiproliferative activity and low toxicity of the &lt;em&gt;T. sericea &lt;/em&gt;methanolic and aqueous leaf extracts extracts against HeLa and Caco2 cancer cell lines indicates their potential in the treatment and prevention of some cancers.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">408</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;BiYun Gu&lt;sup&gt;1,2&lt;/sup&gt;, Joseph Shalom&lt;sup&gt;1,3&lt;/sup&gt;, Ian E. Cock&lt;sup&gt;1,3* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;School of Natural Sciences, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland 4111, AUSTRALIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, CHINA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland 4111, AUSTRALIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bosco Lawarence</style></author><author><style face="normal" font="default" size="100%">Murugan K</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comprehensive Evaluation of Antioxidant Potential of Selected Osbeckia species and their in vitro Culture, Purification and Fractionation</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anthocyanins</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant Capacity</style></keyword><keyword><style  face="normal" font="default" size="100%">Free Radicals</style></keyword><keyword><style  face="normal" font="default" size="100%">Osbeckia Spp.</style></keyword><keyword><style  face="normal" font="default" size="100%">Reactive Oxygen Species</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">July 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">/files/pj-9-5/10.5530pj.2017.5.107/index.html</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">674-682</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Health-benefit properties of natural pigments have been intensely studied, especially the anthocyanins. In the last few decades, research on anthocyanins has attracted biologists by the increasing evidence of their health beneficial effects. &lt;em&gt;Osbeckia,&lt;/em&gt; belongs to Melastomataceae and is well-known for colouring pigments and other bioactive compounds. In the present study, total anthocyanin and antioxidant capacity indicators were evaluated from 8 &lt;em&gt;Osbeckia&lt;/em&gt; spp. and anthocyanin was extracted from &lt;em&gt;in vitro&lt;/em&gt; cultures of &lt;em&gt;O. aspera&lt;/em&gt; and &lt;em&gt;O. reticulata&lt;/em&gt;. Materials and &lt;strong&gt;Methods:&lt;/strong&gt; The antioxidant effect was studied using ABTS (2, 2&amp;rsquo;-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid) radical cation decolourisation assay, the FRAP, the scavenging ability of hydroxyl radicals and the superoxide anion scavenging activity. Anthocyanin extracted from &lt;em&gt;in vitro&lt;/em&gt; cultures were purified and fractionated using column chromatography and LC-MS MS analysis. &lt;strong&gt;Results:&lt;/strong&gt; &lt;em&gt;In vitro&lt;/em&gt; cultures of &lt;em&gt;O. aspera&lt;/em&gt; was obtained in MS medium fortified with various combinations of Benzyl Adenine (BA), Naphthalene acetic acid (NAA) and 2, 4-D. The chromatograms of &lt;em&gt;O. aspera&lt;/em&gt; revealed the presence of malvidin-3 -diglucoside, peonidin, delphinidin and cyanindin whereas &lt;em&gt;O. reticulata &lt;/em&gt;cultures accumulated large amounts of malvidin, cyanindin and cyanidin aglycone. The purified anthocyanins of these species were evaluated for their antioxidant potential and was found more remarkable than the crude extracts. &lt;strong&gt;Conclusion&lt;/strong&gt;: &lt;em&gt;Osbeckia&lt;/em&gt; species are rich in anthocyanin and therefore display potential AOX power. &lt;em&gt;O. aspera&lt;/em&gt; and &lt;em&gt;O. reticulata&lt;/em&gt; callus was induced &lt;em&gt;in vitro&lt;/em&gt; production of anthocyanins. The pool of anthocyanins was purified and fractionated by LCMS/ MS and AOX assays were performed with the purified anthocyanin which showed higher level activities.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">674</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Bosco Lawarence and Murugan K &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Plant Biochemistry and Molecular Biology Laboratory, University College, Trivandrum, Kerala, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>