<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Carla Y. Requejo-Rodríguez</style></author><author><style face="normal" font="default" size="100%">Elmer M. Roncal-Alayo</style></author><author><style face="normal" font="default" size="100%">Carmen R. Silva-Correa</style></author><author><style face="normal" font="default" size="100%">Víctor E. Villarreal-La Torre</style></author><author><style face="normal" font="default" size="100%">William A. Sagástegui-Guarniz</style></author><author><style face="normal" font="default" size="100%">William A. Sagástegui-Guarniz</style></author><author><style face="normal" font="default" size="100%">Walter E. Janampa-Castillo</style></author><author><style face="normal" font="default" size="100%">José E. Alvarez- Trujillo</style></author><author><style face="normal" font="default" size="100%">Glenda J. Vela-Urbina</style></author><author><style face="normal" font="default" size="100%">Abhel A. Calderón-Peña</style></author><author><style face="normal" font="default" size="100%">Cinthya L. Aspajo-Villalaz</style></author><author><style face="normal" font="default" size="100%">María E. Cotrina-León</style></author><author><style face="normal" font="default" size="100%">Julio A. Castañeda-Carranza</style></author><author><style face="normal" font="default" size="100%">Deivy Y. Dionicio-Rosado</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Juglans regia L.: Source of Bioactive Compounds with Potential Anticancer Activity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Angiogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Antitumor</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Juglans regia L.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">998-1003</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;em&gt;Juglans regia&lt;/em&gt; L., commonly known as “walnut”, belongs to the Juglandaceae family, with antioxidant, anti-inflammatory, and hypoglycemic medicinal properties. &lt;strong&gt;Objective:&lt;/strong&gt; Describe the anticancer potential of the bioactive compounds present in &lt;em&gt;Juglans regia&lt;/em&gt; L.&lt;strong&gt; Method:&lt;/strong&gt; Recent scientific studies were reviewed on the effects of bioactive compounds from &lt;em&gt;Juglans regia&lt;/em&gt; L. on inhibiting tumor growth and cancer development in several experimental models. To do this, a scientific literature search was carried out, using databases such as PubMed, Scopus, and Science Direct. &lt;strong&gt;Results:&lt;/strong&gt; Regarding the selected articles, it was found that some bioactive compounds from&lt;em&gt; Juglans regia &lt;/em&gt;L. exhibit mechanisms of anticancer action, among which the following stand out: induction of apoptosis, suppression of angiogenesis, and modulation of cell signaling pathways related to cell proliferation and survival. &lt;strong&gt;Conclusion: &lt;/strong&gt;It is concluded that &lt;em&gt;Juglans regia&lt;/em&gt; L. contains active metabolites with potential anticancer effects.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">998</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Carla Y. Requejo-Rodríguez¹, Elmer M. Roncal-Alayo¹, Carmen R. Silva-Correa&lt;sup&gt;2,*&lt;/sup&gt;, Víctor E. Villarreal-La Torre&lt;sup&gt;2&lt;/sup&gt;, William A. Sagástegui-Guarniz&lt;sup&gt;2&lt;/sup&gt;, César D. Gamarra-Sánchez&lt;sup&gt;2&lt;/sup&gt;, Walter E. Janampa-Castillo&lt;sup&gt;3&lt;/sup&gt;, José E. Alvarez-Trujillo&lt;sup&gt;3&lt;/sup&gt;, Glenda J. Vela-Urbina&lt;sup&gt;3&lt;/sup&gt;, Abhel A. Calderón- Peña&lt;sup&gt;4&lt;/sup&gt;, Cinthya L. Aspajo- Villalaz&lt;sup&gt;4&lt;/sup&gt;, María E. Cotrina-León&lt;sup&gt;5&lt;/sup&gt;, Julio A. Castañeda-Carranza&lt;sup&gt;5&lt;/sup&gt;, Deivy Y. Dionicio-Rosado&lt;sup&gt;6&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Escuela de Posgrado, Universidad Nacional de Trujillo, Perú&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo, Perú&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Facultad de Educación y Ciencias de la Comunicación, Universidad Nacional de Trujillo, Perú&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo, Perú.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Facultad de Ciencias Físicas y Matemáticas, Universidad Nacional de Trujillo, Perú.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Facultad de Ciencias Sociales y Humanidades, Universidad Nacional Ciro Alegría, Perú&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Eko Fuji Ariyanto</style></author><author><style face="normal" font="default" size="100%">Hermin Aminah Usman</style></author><author><style face="normal" font="default" size="100%">Ristaniah Rose Effendy</style></author><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The effect of Sinensetin and Imperatorin on A-549 lung cancer cell viability in vitro</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell viability</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperatorin</style></keyword><keyword><style  face="normal" font="default" size="100%">Sinensetin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">38-46</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Lung cancer remains the leading cause of cancer death worldwide, so research is ongoing to discover new therapeutics, such as plant-derived bioactive compounds. For example, Sinensetin, a plant-derived polymethoxylated flavonoid, and Imperatorin, a natural furanocoumarin, have anti-cancer properties. This study assessed the effects of sinensetin and imperatorin separately and in combination on A-549 lung cancer cell viability. &lt;strong&gt;Method: &lt;/strong&gt;The A-549 lung cancer cell line was treated with sinensetin (60 μM), imperatorin (30 M), or a combination of both compounds (Sin:Imp 30:30 μM; 50:50 μM and 60:30 μM) for 48 hours. Cell viability was then assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and apoptosis was determined using fluorescein isothiocyanate (FITC) Annexin-V/Propidium iodide staining. &lt;strong&gt;Results: &lt;/strong&gt;The combination treatment of Sin:Imp 50:50 and Sin:Imp 60:30 μM reduced cell viability more than the individual treatment of sinensetin and imperatorin, with the lowest cell viability observed for the combination treatment of Sin:Imp 50:50 μM. Likewise, the combination treatment of Sin:Imp 50:50 μM induced the most apoptosis compared to individual treatment. &lt;strong&gt;Conclusion:&lt;/strong&gt; Sinensetin and imperatorin can decrease A-549 lung cancer cell viability and are potent apoptotic inducers, especially when they are used in combination, therefore they are potential lung cancer therapeutics.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article </style></work-type><section><style face="normal" font="default" size="100%">38</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Raden Anita Indriyanti&lt;sup&gt;1,2,*&lt;/sup&gt;, Eko Fuji Ariyanto&lt;sup&gt;3&lt;/sup&gt;, Hermin Aminah Usman&lt;sup&gt;4&lt;/sup&gt;, Ristaniah Rose Effendy&lt;sup&gt;5&lt;/sup&gt;, Diah Dhianawaty&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Bandung Islamic University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Doctoral Program in Medical Science, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department Pathology Anatomy, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Radiology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Amelia Shinta Prasetya</style></author><author><style face="normal" font="default" size="100%">Evelyn Komaratih</style></author><author><style face="normal" font="default" size="100%">Wimbo Sasono</style></author><author><style face="normal" font="default" size="100%">Mercia Chrysanti</style></author><author><style face="normal" font="default" size="100%">Maria Debora Niken Larasati</style></author><author><style face="normal" font="default" size="100%">I Ketut Sudiana</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Intravitreal Resveratrol as Anti Apoptotic Agent Against Retinal  Ganglion Cell Loss in Ischemic Reperfusion Injury</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Glaucoma</style></keyword><keyword><style  face="normal" font="default" size="100%">Ischemic-reperfusion injury</style></keyword><keyword><style  face="normal" font="default" size="100%">Neuroprotective</style></keyword><keyword><style  face="normal" font="default" size="100%">Resveratrol</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">1207-1212</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Glaucoma is an optic neuropathy caused by the apoptosis of retinal ganglion cells and results in progressive retinal ganglion cell injury. A decrease in intraocular pressure (IOP) is a modifiable risk factor for slowing the progression of the disease, and can be accomplished through medication, laser therapy, or surgery. Even though the intraocular pressure has decreased and attained normal levels, the injury to the retinal ganglion cells continues in some cases. It is believed that neuroprotective administration has a positive effect on preventing the loss of retinal ganglion cells.&lt;strong&gt; Methods:&lt;/strong&gt; Bax and Caspase-3 expression were measured involving 20 eyeballs of Rattus Norvegicus by immunohistochemistry examination. I-R injury was developed by increasing intraocular pressure (IOP) through the intracameral balanced salt solution (BSS) injection, then lowered after 60 minutes. Samples were divided into 4 groups: control, no further injection group, phosphate-buffered saline (PBS)-injected group and resveratrol-injected group. Each group was enucleated at days 7, 0, 7, and 7, respectively. Data with a non-normal distribution were examined using the Kruskal-Wallis test, and if the outcome was significant, the Mann-Whitney test. &lt;strong&gt;Results:&lt;/strong&gt; The highest mean Bax and Caspase-3 expression was found in PBS injected and enucleated at day 7 group (G2), 0.96±0.40 and 0.72 ± 0.30, respectively. When compared to PBS injection, the expression of Bax and Caspase-3 was lower in the resveratrol-injected group. &lt;strong&gt;Conclusion: &lt;/strong&gt;Bax and Caspase-3 expressions were lower in the intravitreal injection of Resveratrol in the dose of 100 µM following the I-R injury group compared to the group without intravitreal Resveratrol injection.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1207</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Amelia Shinta Prasetya&lt;sup&gt;1&lt;/sup&gt; , Evelyn Komaratih&lt;sup&gt;1,*&lt;/sup&gt;, Wimbo Sasono&lt;sup&gt;1&lt;/sup&gt; , Mercia Chrysanti&lt;sup&gt;1&lt;/sup&gt; , Maria Debora Niken Larasati&lt;sup&gt;1&lt;/sup&gt; , I Ketut Sudiana&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Hospital, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Departement of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Janany Manimaran</style></author><author><style face="normal" font="default" size="100%">Daruliza Kernain Mohd Azman</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">RETRACTED: The Effects of Andrographolide on Apoptosis in PC-3 Cell Line Via the Involvement of Caspases 3, 8 And 9</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Andrographolide</style></keyword><keyword><style  face="normal" font="default" size="100%">Anti-cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Caspase.</style></keyword><keyword><style  face="normal" font="default" size="100%">PC-3 cell line</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">612-621</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;The Article has been Retracted based on the Authors' Request.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">612</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Janany Manimaran, Daruliza Kernain Mohd Azman*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Institute for Research in Molecular Medicine, University of Science Malaysia, 11800 Gelugor, Pulau Pinang, MALAYSIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ricadonna Raissa</style></author><author><style face="normal" font="default" size="100%">Anna Safitri</style></author><author><style face="normal" font="default" size="100%">Masruri Masruri</style></author><author><style face="normal" font="default" size="100%">Ma Asuncion Guiang Beltran5</style></author><author><style face="normal" font="default" size="100%">Aulanni’am Aulanni’am</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">An In Silico Study of Examining Bioactive Compounds from Azadirachta indica Juss. (Neem) as Potential Death Receptor 5 Inductor in Hepatoma Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Azadirachta indica Juss.</style></keyword><keyword><style  face="normal" font="default" size="100%">Cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Death receptor 5</style></keyword><keyword><style  face="normal" font="default" size="100%">in silico</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">April 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">343-349</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Hepatocellular carcinoma is a disease that occurs due to the uncontrolled growth of abnormal hepatocytes. While cancer cells will not die by itself, due to resistance to death receptors 5 (DR5)-mediated apoptosis. This study is aimed to investigate&lt;em&gt; Azadirachta indica&lt;/em&gt; Juss. leaves compound, such as gedunin and nimbolide, in binding DR5 and stimulated the TNF-related apoptosis inducing ligand (TRAIL), native ligand binding to DR5, which has a role of pro-apoptotic by docking simulation. The ligand and protein preparations were done using Discovery Studio 2016 and Hex 8.0.0 for docking. Visualization was done using Discovery Studio 2016. The docking studies revealed that nimbolide has a lower binding energy with the DR5-TRAIL complex than gedunin. According to the findings, nimbolide is a more effective DR5-TRAIL binding inducer than gedunin and has a higher binding affinity for DR5-TRAIL. This interaction has the potential to significantly reduce DR5-TRAIL binding resistance. Nimbolide and gedunin can be considered as drugs that can sensitize TRAIL binding to DR5 and increase the activation of one of hepar cancers signaling apoptosis pathways.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Research Article </style></work-type><section><style face="normal" font="default" size="100%">343</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ricadonna Raissa&lt;sup&gt;1&lt;/sup&gt;, Anna Safitri&lt;sup&gt;2,3&lt;/sup&gt;, Masruri Masruri&lt;sup&gt;2&lt;/sup&gt;, Ma Asuncion Guiang Beltran&lt;sup&gt;5&lt;/sup&gt;, Aulanni’am Aulanni’am&lt;sup&gt;2,4&lt;/sup&gt;,&lt;sup&gt;*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program of Chemistry, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Malang, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Malang, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Research Center for Smart Molecules of Natural Genetic Resources (SMONAGENES), Universitas Brawijaya, Malang, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;College of Veterinary Medicine, Tarlac Agricultural University, Camiling, Tarlac, PHILIPPINES.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Titiek Sumarawati</style></author><author><style face="normal" font="default" size="100%">Chodidjah</style></author><author><style face="normal" font="default" size="100%">Taufiqurrachman Nasihun</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Both Ethanol and Ethyl Acetate Curcuma Zedoaraia Extract was Capable of Inducing Cells Death in T47D Cell Line Culture</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetyl acetate</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Curcuma zedoaria</style></keyword><keyword><style  face="normal" font="default" size="100%">Ethanol</style></keyword><keyword><style  face="normal" font="default" size="100%">Necrosis</style></keyword><keyword><style  face="normal" font="default" size="100%">T47D cell line</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">737-743</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;&lt;em&gt;Curcuma zedoaria&lt;/em&gt; (CZ) has been proven capable of inducing apoptosis in cells cancer. CZ extraction can be performed by ethanol and acetyl acetate as solvent. However, which one of these extracts is superior remains unclear.&lt;strong&gt; Objective:&lt;/strong&gt; This study aimed to investigate the difference potential effect of ethanol and acetyl CZ extract on apoptosis of T47D cell line. &lt;strong&gt;Methods:&lt;/strong&gt; In this study 21 wells were assign into seven groups: control group (T47D); treatment groups consisting of group of ethanol CZ extract 46 (EtZ-46); group of ethanol CZ extract 23 (EtZ-23); group of ethanol CZ extract 11 (EtZ-11); and group of ethyl acetate CZ extract 111 (AcZ-111); group of ethyl acetate CZ extract 55 (AcZ-55); and group of ethyl acetate CZ extract 27 (AcZ-27). In T47D group only loaded with T47D cell line; in treatment groups aside from loaded with T47D cell line culture, also treated with ethanol or acetyl acetate CZ extract respectively. Concentration of T47D cell was 5 x 10&lt;sup&gt;4&lt;/sup&gt; T47D cells line in 100 μl suspension loaded on each well of 21 wells and kept in CO&lt;sub&gt;2&lt;/sub&gt; incubator overnight. The apoptosis cells were measured after 48 hours post CZ treatment. &lt;strong&gt;Results: &lt;/strong&gt;Post Hoc analysis indicated that the number of apoptosis cells in AcZ-111 was significant higher compared to that of other groups, p&amp;lt;0.05. &lt;strong&gt;Conclusion:&lt;/strong&gt; Acetyl acetate CZ extract treatment with dose 111 μg was capable of inducing apoptosis in T47D cell line superior than that of other groups including ethanol CZ extract.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">737</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Titiek Sumarawati&lt;sup&gt;1,&lt;/sup&gt;*, Chodidjah&lt;sup&gt;2&lt;/sup&gt;, Taufiqurrachman Nasihun&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Chemistry, Medical Faculty, Sultan Agung Islamic University, Central Java – 50112, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Anatomy, Medical Faculty, Sultan Agung Islamic University, Central Java – 50112, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Biochemistry, Sultan Agung Islamic University, Semarang, Central Java – 50112, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Arsyik Ibrahim</style></author><author><style face="normal" font="default" size="100%">Siswandono</style></author><author><style face="normal" font="default" size="100%">Bambang Prajogo EW</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic Activity of Peronema canescens Jack Leaves on Human Cells: HT-29 and Primary Adenocarcinoma Colon Cancer</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell cycle</style></keyword><keyword><style  face="normal" font="default" size="100%">Colon cancer cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Necrosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Peronema canescens Jack</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">1389-1396</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;In Indonesia, this species was well known in Sumatera, Kalimantan, Java, and Sulawesi. &lt;em&gt;Peronema canescens &lt;/em&gt;Jack (Sungkai) was traditionally used as an anti-flatulent, fever, toothache. Sungkai leaves contain many secondary metabolites with potential anticancer activity. The reported anticancer research was still limited to the cytotoxic activity of chloroform extract on the HT-29 colon cancer cell line. However, it was necessary to uncover the underlying mechanism. &lt;strong&gt;Aim&lt;/strong&gt;: The purpose of this study was to investigate the mechanism (such as cell cycle inhibition, induces cells apoptosis, and necrosis) of subfraction chloroform (SF3) from P. canescens extract has anticancer activity on HT-29 cells and primary Adenocarcinoma (AdenoCa pT3N1cM1) colon cancer cells. &lt;strong&gt;Materials and Methods&lt;/strong&gt;: The extraction by maceration method using methanol solvent, the fractionation process was using vacuum column chromatography (VCC) with polarity gradient eluent. The cytotoxicity of SF3 was measured by MTT assay. The cell cycle inhibition, apoptosis induction, and necrosis cells were evaluated with the Flow cytometry method. &lt;strong&gt;Results&lt;/strong&gt;: Cytotoxicity value (IC&lt;sub&gt;50&lt;/sub&gt;) against AdenoCa cells was 1.897 μg/ml. The inhibition activity of synthesis and mitosis phase in cell cycle demonstrated that the different concentrations of SF3 have inhibition activity on HT-29 (29.614 μg/ml) of 26.79% and 0.16%, AdenoCa cells (14.807 μg/ml) of 10.27% and 19.29%, respectively. For induced apoptosis activity on HT-29 (29.614 μg/ml) and AdenoCa cells (14.807 μg/ml) were 26.58% and 11.50%, successively. Whereas, necrosis activity on HT-29 (29.614 μg/ ml) and AdenoCa cells (14.807 μg/ml) were 0.02%, and 9.56%, respectively. Conclusion: The subfractions chloroform (SF3) of P. canescens extract has potential activity on HT-29 and Adenocarcinoma cells through cell cycle inhibition, induces apoptosis and necrosis cells.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1389</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Arsyik Ibrahim&lt;sup&gt;1,2,*&lt;/sup&gt;, Siswandono&lt;sup&gt;3&lt;/sup&gt;, Bambang Prajogo EW&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program, Faculty of Pharmacy, University of Airlangga, Surabaya 60115, East Java, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Mulawarman, Samarinda 75119, East Kalimantan, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Airlangga, Surabaya 60115, East Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Misgiati Misgiati</style></author><author><style face="normal" font="default" size="100%">Aty Widyawaruyanti</style></author><author><style face="normal" font="default" size="100%">Sentot Joko Raharjo</style></author><author><style face="normal" font="default" size="100%">Sukardiman Sukardiman</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Ergosterol Isolated from Agaricus blazei Murill N-Hexane Extracts as Potential Anticancer MCF-7 Activity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agaricus blazei</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Egosterol</style></keyword><keyword><style  face="normal" font="default" size="100%">MCF-7 cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Murill extract</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">418-426</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Extracts and some of the &lt;em&gt;Agaricus blazrei &lt;/em&gt;Murill isolates have potential anticancer. Ergosterol isolate from &lt;em&gt;Amaouroderma rude&lt;/em&gt; can also inhibit the growth of MDA-MB-231 cancer cells through apoptotic pathways by increasing FOXO&lt;sub&gt;3&lt;/sub&gt; expression, while its potency against MCF-7 cells has not been reported. The purpose of this study was to isolate, determine the structure, determine the anticancer activity of MCF-7 cells, and the isolate mechanism by apoptosis from one of isolates the n-hexane &lt;em&gt;A.blazei &lt;/em&gt;Murill extracts. This research method includes the isolation of compounds from &lt;em&gt;A.blazei &lt;/em&gt;Murill extract by chromatography method guided using Bioactivity Guided Isolation. The structure elucidation of structure isolates used UV, NMR and MS spectroscopy. Anticancer activity test using the MTT cytotoxic test. Eludation of UV, NMR and MS structures showed a ergostrerol. The anticancer activity test showed IC&lt;sub&gt;50&lt;/sub&gt; values of 43.10 μg/ mL with the strong cytotoxic category. The mechanism of action is to increase apoptosis induction through inhibition of the cell cycle in the G2/ M phase. The conclusion of the isolated compound was ergosterol with an IC&lt;sub&gt;50&lt;/sub&gt; value of 43.10 μg / mL with an increased apoptosis induction mechanism through inhibition of the cell cycle in the G2/ M phase.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">418</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Misgiati Misgiati&lt;sup&gt;1,2&lt;/sup&gt;, Aty Widyawaruyanti&lt;sup&gt;3&lt;/sup&gt;, Sentot Joko Raharjo&lt;sup&gt;2&lt;/sup&gt;, Sukardiman Sukardiman&lt;sup&gt;4,&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Post Graduate Student of Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya 6011, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Academic of Pharmacy and Food Analyst of Putra Indonesia Malang, Jl. Barito 5 Malang 65123, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Natural Product Medicine Research and Development, Institute of Tropical Disease, Airlangga University, Surabaya 6011, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya 6011, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Illyana Ismail</style></author><author><style face="normal" font="default" size="100%">Rapeah Suppian</style></author><author><style face="normal" font="default" size="100%">Habsah Mohamad</style></author><author><style face="normal" font="default" size="100%">Siti Aisha Mohd Radzi</style></author><author><style face="normal" font="default" size="100%">Hasmah Abdullah</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Cytotoxicity and Apoptosis-inducing Activity of Quercus infectoria Extracts in HeLa Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell cycle</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus infectoria</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">401-410</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;Quercus infectoria&lt;/em&gt; galls (QI) extracts were previously reported to have cytotoxicity effects towards human cervical cancer cells, HeLa. However, the underlying molecular mechanisms of the extracts have been poorly determined. &lt;strong&gt;Objective: &lt;/strong&gt;The present study was undertaken to examine the effect of ethyl acetate extracts of QI (EAQI) on cell cytotoxicity and induction of apoptosis in HeLa cells. &lt;strong&gt;Materials and Method: &lt;/strong&gt;The &lt;em&gt;in vitro &lt;/em&gt;cytotoxicity was investigated by using the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay and the OD values were read at 570 nm. Meanwhile the induction of apoptosis was measured by using acridine orange and propidium iodide (AO/PI) staining, flow cytometry analysis of annexin V/PI staining and cell cycle distribution.&lt;strong&gt; Results: &lt;/strong&gt;MTT assay showed that EAQI exhibited cytotoxicity effect on HeLa cells with IC&lt;sub&gt;50 &lt;/sub&gt;of 11.50 ± 0.50 μg/ml. HeLa cells underwent apoptosis in response to EAQI treatment, demonstrated by an increase in the percentage of apoptotic cell stained with AOPI from 1.00% to 10.33% compared to untreated cell population (&lt;em&gt;p&lt;/em&gt;&amp;lt;0.05) at 72 hours of treatment. The evidence of early apoptosis in treated cells were also observed in annexin V/PI staining. Furthermore, an increase of cell population in sub G0/G1 phase revealed that apoptosis as the mode of cell death in HeLa cells treated with EAQI. &lt;strong&gt;Conclusion: &lt;/strong&gt;These findings indicated that EAQI significantly inhibits HeLa cell growth through induction of apoptosis. Further studies are needed to confirm the mechanism of cell death by expression of apoptotic cascade in HeLa cells treated with EAQI.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">401</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Illyana Ismail&lt;sup&gt;1&lt;/sup&gt;, Rapeah Suppian&lt;sup&gt;2&lt;/sup&gt;, Habsah Mohamad&lt;sup&gt;3&lt;/sup&gt;, Siti Aisha Mohd Radzi&lt;sup&gt;3&lt;/sup&gt;, Hasmah Abdullah&lt;sup&gt;2,&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;School of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Gong Badak Campus, 21300 Kuala Terengganu, Terengganu, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Biomedicine Programme, School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian Kelantan, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21300 Kuala Terengganu, Terengganu, MALAYSIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Medha A. Bhat</style></author><author><style face="normal" font="default" size="100%">Hosakatte Niranjana Murthy</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation of Andrographolide from Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke and its Anticancer Activity against Human Ovarian Teratocarcinoma</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Andrographis</style></keyword><keyword><style  face="normal" font="default" size="100%">Andrographolide</style></keyword><keyword><style  face="normal" font="default" size="100%">Anticancer activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Ovarian teratocarcinoma</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">660-668</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Andrographolide is a well-known anticancer phytochemical often isolated from &lt;em&gt;Andrographis paniculata&lt;/em&gt; (Burm. f.) Nees. (Acanthaceae). Though &lt;em&gt;Andrographis lineata&lt;/em&gt; Wall. ex Nees var. lawii C.B. Clarke (ALw) which also belongs to the same family has an adequate amount of andrographolide; remained untouched for isolation of andrographolide and anticancer studies. Therefore, this study was targeted to isolate the andrographolide from the leaves of ALw and to assess its role inthe induction of apoptosis against the human ovarian teratocarcinoma (PA-1) cell line. &lt;strong&gt;Methods: &lt;/strong&gt;Column chromatography, thin-layer chromatography (TLC), preparative TLC were used for the isolation and purification while melting point, ultraviolet (UV)-visible spectroscopy, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H NMR), carbon-13 (C13) nuclear magnetic resonance (&lt;sup&gt;13&lt;/sup&gt;C NMR) analysis were carried out for characterization of the compound. 3-(4,5-dimethylthiaxo-2yl) 2, 5-diphenyl tetrazolium bromide (MTT) assay was carried out for cytotoxicity test and further Annexin-V staining, caspase 3 activity, B-cell lymphoma-2 (Bcl-2) activity, cell cycle analysis, and DNA damage study by terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) assays were carried out for apoptosis study. &lt;strong&gt;Results:&lt;/strong&gt; Andrographolide was isolated from the methanolic extract of leaves of ALw which had a melting point of 230 ºC, λ&lt;sub&gt;max&lt;/sub&gt; at 223 nm. FTIR results proved the presence of hydroxyl group, alkanes, carbon-carbon double bond, and a characteristic gamma lactone carbonyl. NMR data confirmed the 20 carbon structure. In the MTT assay cytotoxicity against PA-1 was at 3.7 μg/ml with other apoptotic assays supporting the induction of apoptosis by the compound at that concentration. &lt;strong&gt;Conclusion:&lt;/strong&gt; ALw is proved to be an alternate source of andrographolide with potential abilities to induce apoptosis in ovarian cancer cells.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">660</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Medha A. Bhat, Hosakatte Niranjana Murthy* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Department of Botany, Karnatak University, Dharwad 580003, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kusmardi Kusmardi</style></author><author><style face="normal" font="default" size="100%">Elvan Wiyarta</style></author><author><style face="normal" font="default" size="100%">Ari Estuningtyas</style></author><author><style face="normal" font="default" size="100%">Nurhuda Sahar</style></author><author><style face="normal" font="default" size="100%">Yurnadi Hanafi Midoen</style></author><author><style face="normal" font="default" size="100%">Aryo Tedjo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Inflammation</style></keyword><keyword><style  face="normal" font="default" size="100%">Mahkota Dewa</style></keyword><keyword><style  face="normal" font="default" size="100%">Ulcerative colitis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">23-29</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Ulcerative colitis (UC) is a part of incurable chronic inflammatory disease that has gained importance over the past few decades. A lot of research has been done to find effective treatments for UC, one of which is herbal medicine. &lt;em&gt;Phaleria macrocarpa&lt;/em&gt; (PM), an Indonesian native plant, is thought to be an alternative therapy for UC because of its anti-inflammatory properties. Therefore, in this research, &lt;em&gt;Phaleria macrocarpa&lt;/em&gt; Leaves Ethanol Extract (&lt;em&gt;PM&lt;/em&gt;LEE) is used to assess its effect on UC by using Caspase-3 as apoptosis marker. PMLEE was made from dried material of PM that undergo maceration. Animals were separated into six groups: normal, negative control, positive control, and PMLEE groups (100, 200, 300 mg/kgBW). PMLEE was then injected to BALB/c mice that have been induced by dextran sodium sulphate (DSS) for 7 consecutive days. DSS is used to model UC in mice colon tissue. All animals were sacrificed and their colons were collected then stained with anti-Caspase-3. The stained sections were subsequently examined with ImageJ based on color intensity which generated H-Score as the results. Based on H-Score of each group, PMLEE 300mg has significantly upregulate the expression of Caspase-3 compare to the negative control (p=0.015). PMLEE also has a tendency to be dose dependent based on the significant difference between PMLEE doses. Therefore, it concludes that PMLEE is able to upregulate the expression of Caspase-3 in colon cells as in this study it was directly proportional. &lt;strong&gt;Key words:&lt;/strong&gt; Mahkota Dewa, Inflammation, Apoptosis, Ulcerative colitis.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">23</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Kusmardi Kusmardi&lt;sup&gt;1&lt;/sup&gt;, Elvan Wiyarta&lt;sup&gt;2,&lt;/sup&gt;*, Ari Estuningtyas&lt;sup&gt;3&lt;/sup&gt;, Nurhuda Sahar&lt;sup&gt;4&lt;/sup&gt;, Yurnadi Hanafi Midoen&lt;sup&gt;4&lt;/sup&gt;, Aryo Tedjo&lt;sup&gt;5 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Anatomic Pathology, Drug Development Research Cluster, Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6 Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6 Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6 Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6 Jakarta, INDONESIA&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;5Department Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6 Jakarta, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Okid Parama Astirin</style></author><author><style face="normal" font="default" size="100%">Adi Prayitno</style></author><author><style face="normal" font="default" size="100%">Anif Nur Artanti</style></author><author><style face="normal" font="default" size="100%">Elisa Herawati</style></author><author><style face="normal" font="default" size="100%">Afiyati Nur ‘Aini Saad</style></author><author><style face="normal" font="default" size="100%">Ajeng Dara Firstlia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Single-Dose and Combined-Dose of Nanoparticles from Soursop Leaves (Annona muricata L.) and Sappan Wood (Caesalpinia sappan L.) Induced Apoptosis and Necrosis in HeLA Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Annona muricata L.</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Caesalpinia sappan L.</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Nanoparticles</style></keyword><keyword><style  face="normal" font="default" size="100%">Necrosis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">1134-1142</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Apart from the medical advancement of chemotherapy, various plants were known as beneficial for cancer therapy because they can kill cancer cells selectively without damaging the normal cells. Here, we showed that nanoparticles formulated from chloroform fraction of soursop (&lt;em&gt;Annona muricata&lt;/em&gt; L.) leaves and ethyl acetate fraction of sappan wood (&lt;em&gt;Caesalpinia sappan&lt;/em&gt; L.) have anti-proliferative and cytotoxic effects on HeLa cervical cancer cells. &lt;strong&gt;Methods: &lt;/strong&gt;The cytotoxic effect was evaluated using a single dose of each nanoparticle and a combined dose to obtain a synergistic effect. The mechanism of induced cell death via apoptosis or necrosis pathway was evaluated using flow cytometry by incorporating Annexin V and propidium iodide.&lt;strong&gt; Results: &lt;/strong&gt;Synthesis of nanoparticles from the extract of soursop leaves (nano-SL) and extract of sappan wood (nano-SW) yielded particle sizes ranging from 248 to 317 nm. Nano-SL and nano-SW decreased the viability of HeLa cervical cancer cells in a dose-dependent manner with IC&lt;sub&gt;50 &lt;/sub&gt;values of 63,32 μg/ml dan 40,88 μg/ml, respectively. The combined dose of 1/8 IC&lt;sub&gt;50&lt;/sub&gt; from both nanoparticles showed a strong synergistic effect, as shown by the combination index value of 0.13 based on the same mode of action and different modes of action. In HeLa cells treated with a combined dose of nanoparticles, the total apoptotic cells increased two times greater than that in control cells. &lt;strong&gt;Conclusion: &lt;/strong&gt;Nano-SL and nano-SW induce apoptosis and necrosis in HeLa cells. Combined-dose of both nanoparticles produced a synergistic effect that could reduce the amount of the required individual dose while increasing the total effect.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1134</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Okid Parama Astirin&lt;sup&gt;1&lt;/sup&gt;, Adi Prayitno&lt;sup&gt;2&lt;/sup&gt;, Anif Nur Artanti&lt;sup&gt;3&lt;/sup&gt;, Elisa Herawati&lt;sup&gt;1,&lt;/sup&gt;*, Afiyati Nur ‘Aini Saad&lt;sup&gt;1&lt;/sup&gt;, Ajeng Dara Firstlia&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Surakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pathobiology, Faculty of Medicine, Sebelas Maret University, Surakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacy, Vocational College, Sebelas Maret University, Surakarta, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Selvaraj Jayaraman</style></author><author><style face="normal" font="default" size="100%">Ponnulakshmi Rajagopal</style></author><author><style face="normal" font="default" size="100%">Vishnupriya Veeraraghavan</style></author><author><style face="normal" font="default" size="100%">Poonguzhali Sivagnanam</style></author><author><style face="normal" font="default" size="100%">Divya Ravikumar</style></author><author><style face="normal" font="default" size="100%">Sumetha Suga Deiva Suga</style></author><author><style face="normal" font="default" size="100%">Kavin Mozhi James</style></author><author><style face="normal" font="default" size="100%">Surapaneni Krishna Mohan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Black Horehound (Ballota nigra Linn) Induces Apoptosis in Prostate Cancer Cells (PC-3) Through Intrinsic Signalling Cascade</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Ballota nigra</style></keyword><keyword><style  face="normal" font="default" size="100%">Intrinsic pathway</style></keyword><keyword><style  face="normal" font="default" size="100%">PC3</style></keyword><keyword><style  face="normal" font="default" size="100%">Prostate cancer</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1377-1382</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Prostate cancer is the most commonly diagnosed cancer among men. The disease varies widely in its clinical aggressiveness.&lt;em&gt; Ballota nigra &lt;/em&gt;Linn (Black horehound) is a three-foot, perennial herb of the family Lamiaceae and it has been shown to have various pharmacological properties such as antioxidant, hypoglycemic, neuro-sedative, antibacterial, insecticidal and anticholinesterase activities. However, the elucidation of &lt;em&gt;B.nigra&lt;/em&gt; for its anticancer activity in prostate cancer has not been studied so far. &lt;strong&gt;Methodology: &lt;/strong&gt;Prostate cancer PC3 cells were treated with different concentrations of &lt;em&gt;B.nigra&lt;/em&gt; (50, 100, 200 &amp;amp; 400μg/ml) for the analysis of Bcl-2, Phosphorylation of Bcl2 (p-Bcl2) and tumor suppressor protein p53, Case pase-3 and caspase-9 in PC3 cells. &lt;strong&gt;Results:&lt;/strong&gt; The &lt;em&gt;B.nigra&lt;/em&gt; ethanolic leaf extract reduced the levels of anti apoptotic proteins (Bcl-2, p-Bcl2) and increased the level of tumor suppressor protein p53, caspase-3 and 9 significantly (p&amp;lt;0.05). &lt;strong&gt;Conclusion:&lt;/strong&gt; Results of the study show that &lt;em&gt;B.nigra&lt;/em&gt; has potential anticancer activity by modulating intrinsic activity of apoptotic signaling in PC-3 cells. Thus, &lt;em&gt;B.nigra &lt;/em&gt;may have a potential therapeutic option for the treatment of prostate cancer.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1377</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Selvaraj Jayaraman&lt;sup&gt;1,&lt;/sup&gt;*, Ponnulakshmi Rajagopal&lt;sup&gt;2&lt;/sup&gt;, Vishnupriya Veeraraghavan&lt;sup&gt;1&lt;/sup&gt;, Poonguzhali Sivagnanam&lt;sup&gt;3&lt;/sup&gt;, Divya Ravikumar&lt;sup&gt;4&lt;/sup&gt;, Sumetha Suga Deiva Suga&lt;sup&gt;5&lt;/sup&gt;, Kavin Mozhi James&lt;sup&gt;3&lt;/sup&gt;, Surapaneni Krishna Mohan&lt;sup&gt;6&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), West K. K. Nagar, Chennai-600 078, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Medical Surgical Nursing, Panimalar College of Nursing, Varadharajapuram, Poonamallee, Chennai - 600 123, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Obstetrics and Gynaecology, Panimalar Medical College Hospital &amp;amp; Research Institute, Varadharajapuram, Poonamallee, Chennai - 600 123, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Microbiology, Panimalar Medical College Hospital &amp;amp; Research Institute, Varadharajapuram, Poonamallee, Chennai - 600 123, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Biochemistry, Panimalar Medical College Hospital &amp;amp; Research Institute, Varadharajapuram, Poonamallee, Chennai - 600 123, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Titik Sumarawati</style></author><author><style face="normal" font="default" size="100%">Ignatius Riwanto</style></author><author><style face="normal" font="default" size="100%">Soeharyo Hadisaputro</style></author><author><style face="normal" font="default" size="100%">Edi Dharmana</style></author><author><style face="normal" font="default" size="100%">Taufiqurachman Nasihun</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effect of Phaleria macrocapa on Atrophy and Apoptosis of Intestinal Mucous Cell and Phalerin Concentration at Portal Vein and Systemic Circulation in Adenocarcinoma Mice following Adriamycine and Cyclophosphamide Treatment</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Atrophy</style></keyword><keyword><style  face="normal" font="default" size="100%">Concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">Phalerin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">603-610 </style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Chemotherapy has been proven capable of reducing breast cancer cell progression; however the adverse effect also emerging. Thus, diminish those adverse effects with botanical product &lt;em&gt;Phaleria macrocarpa &lt;/em&gt;(PM) as adjuvant therapy is necessary. &lt;strong&gt;Objective: &lt;/strong&gt;This study aimed to evaluate the effect of PM treatment in combination with &lt;em&gt;adriamycine&lt;/em&gt; and cyclophosphamide (AC) on intestinal apoptosis and their correlation with phalerin concentration in systemic circulation. &lt;strong&gt;Methods: &lt;/strong&gt;In the experimental study, 30 female mice with adenocarcinoma were assign into 5 groups: Neg-G, only given aquadest; Portal vein group (PMV-G) and systemic circulation groups (PMC-G), were administered PM 0.146mg/day; Portal vein group (PMACV-G) and systemic circulation group (PMACC-G), were administered &lt;em&gt;Phaleria macrocarpa &lt;/em&gt;0,146 mg orally, &lt;em&gt;Adriamycine &lt;/em&gt;0,013 mg and Cyclophosphamide 0,0156 mg singgle dose intravenously. Phalerin concentration was measured by HPLC methods at minute 30, 60, 90, 120, 150, and 180 after treatment. At the end of study, intestinal mucous cell apoptosis was identified by TUNEL methods. &lt;strong&gt;Results: &lt;/strong&gt;independent t test analyses showed that index of apoptosis of intestinal mucous cell were significant higher in PMAC-G compared to that of Neg-G and PM-G, p &amp;lt; 0.05. In contrary, phalerin concentration in PMAC-G was significant lower compared to that of PM-G, p &amp;lt; 0.05. The Pearson analysis indicated the inverse correlation (r= -736, p&amp;gt;0.05) between apoptosis index with phalerin concentration.&lt;strong&gt; Conclusion: &lt;/strong&gt;Treatment of PM in combination with AC has been proven able to increase intestinal mucous cell apoptosis and decrease phalerin concentration. However, the inverse correlation didnot exist.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">603</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Titik Sumarawati&lt;sup&gt;1,&lt;/sup&gt;*, Ignatius Riwanto&lt;sup&gt;2&lt;/sup&gt;, Soeharyo Hadisaputro&lt;sup&gt;3&lt;/sup&gt;, Edi Dharmana&lt;sup&gt;4&lt;/sup&gt;, Taufiqurachman Nasihun&lt;sup&gt;5&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Chemistry Medical Faculty of Sultan Islamic Agung University, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Surgery, Medical Faculty of Diponegoro University, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Internal Medicine Medical Faculty of Diponegoro University, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Parasitology Medical Faculty of Diponegoro University, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Biochemistry Medical Faculty of Sultan Agung Islamic University, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Lal Chand Pal</style></author><author><style face="normal" font="default" size="100%">Anil kumar</style></author><author><style face="normal" font="default" size="100%">Veena Pande</style></author><author><style face="normal" font="default" size="100%">Ch V Rao</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Hepatoprotective Effect of Bioactive Fraction of Lagerstroemia speciosa (L.) Pers. Bark Against Monosodium Glutamate-Induced Liver Toxicity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Lagerstroemia speciosa</style></keyword><keyword><style  face="normal" font="default" size="100%">Monosodium glutamate</style></keyword><keyword><style  face="normal" font="default" size="100%">Superoxide dismutase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1630-1640</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; The phenolics and flavanoid enriched bioactive fraction of &lt;em&gt;L. speciosa&lt;/em&gt; bark were reported for its medicinal value in various illness however hepatoprotective activity against monosodium glutamate-induced liver toxicity yet to be reported. &lt;strong&gt;Objective:&lt;/strong&gt; To evaluate the hepatoprotective and antioxidant potential of &lt;em&gt;L. speciosa&lt;/em&gt; bark extract fraction against monosodium glutamate-induced liver toxicity. &lt;strong&gt;Methods: &lt;/strong&gt;The phytochemical constituent of ethyl acetate fraction of &lt;em&gt;L. speciosa&lt;/em&gt; bark extract (LSE) were identified by GC-MS analysis. The antioxidant activity of LSE were analyzed with &lt;em&gt;in-vitro&lt;/em&gt; antioxidant assay and subjected to evaluate hepatoprotective activity against monosodium glutamate induced liver toxicity in rat. &lt;strong&gt;Results:&lt;/strong&gt; LSE evaluated as rich in phenolics and flavonoid content along with potent hepatoprotective activity. GC-MS analysis of bioactive fraction exhibits Palmitic Acid, Octadecanoic acid, 5-methyluridine, catechine, epigallocatechin, and norgestrel as major biologically active phytocompounds. Oral administration of LSE (100 and 200 mg/kg.) declined the elevated levels of the biochemical marker as well as interleukins while enhanced the enzymatic antioxidant activity and reduced the increased level of stress marker (MDA) in monosodium glutamate-induced rats. It also restored the altered expression level of proapoptotic genes, but there is no significant change in the expression level of the anti-apoptotic gene. LSE improved histopathology of the liver through the improvement of hepatocellular architecture, inflammation, and attenuation of vascular and cellular degeneration. &lt;strong&gt;Conclusion:&lt;/strong&gt; The bioactive fraction of &lt;em&gt;L. speciosa&lt;/em&gt; bark was found to exhibit strong antioxidant and hepatoprotection in monosodium glutamate induced liver toxicity in rats.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1630</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Lal Chand Pal&lt;sup&gt;1,2&lt;/sup&gt;, Anil kumar&lt;sup&gt;1,2&lt;/sup&gt;, Veena Pande&lt;sup&gt;2&lt;/sup&gt;, Ch.V. Rao&lt;sup&gt;1,&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;CSIR-National Botanical Research Institute, Lucknow, UP, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biotechnology, Kumaun University, Nainital, Uttarakhand- 263001, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Taufiqurrachman Nasihun</style></author><author><style face="normal" font="default" size="100%">Eni Widayati</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Pimpinella Treatment on Reducing Apoptosis of Kidney Cells Following UVB Radiation in Rats</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Bax</style></keyword><keyword><style  face="normal" font="default" size="100%">Caspase3</style></keyword><keyword><style  face="normal" font="default" size="100%">Kidney Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Pimpinella alpina Molk</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">503-509 </style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Pimpinella alpina Molk (PM) is a botanical antioxidant was able to inhibit apoptosis in various cells. Apoptosis is a leading cause of tubular atrophy and therefore chronic kidney disease. However, the effect of PM on reducing apoptosis in kidney cells remains unclear. &lt;strong&gt;Objective:&lt;/strong&gt; aim of this study to elucidate the effect of PM on reducing apoptosis in kidney cells. Methods: In the post test only control group design, 35 male rats were grouped into 7 comprise: NC-G, samples were neither exposure to UVB nor PM treatment; NG-7 and NG-15, all samples were only exposure to UVB irradiation for 7 days; P10-7, P15-7, P10-15, P15-15 groups, samples were exposure to UVB for 7 days and treated with PM for 7 and 15 days respectively. Bax and Caspase3 expression were assessed by rt-PCR and IHC staining method. &lt;strong&gt;Results: &lt;/strong&gt;Statistical analysis showed that RNA-Bax and RNA-caspase3, Bax and caspase3 protein expression in P15-7, P10-15 and P15-15 were lower significantly compared to those of NG-7, p&amp;lt;0.05, and no significant difference compared to those of NC-G, p &amp;gt; 0.05.&lt;strong&gt; Conclusion:&lt;/strong&gt; PM treatment with 100 and 150 mg/day for seven and fifteen days were able to decrease Bax and Caspase3 expression in kidney cells following UVB irradiation. Even, the decreased in Bax and caspase3 expression were comparable to normal.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">503</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Taufiqurrachman Nasihun&lt;sup&gt;1,&lt;/sup&gt;*, Eni Widayati&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biochemistry, Medical Faculty, Sultan Agung Islamic University, Semarang, Central Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Chemistry, Medical Faculty, Sultan Agung Islamic University, Central Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Worawat Songjang</style></author><author><style face="normal" font="default" size="100%">Arunya Jiraviriyakul</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Honokiol and Magnolol Induce Apoptosis and Cell Cycle Arrest in Human Ovarian Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Honokiol</style></keyword><keyword><style  face="normal" font="default" size="100%">Magnolol</style></keyword><keyword><style  face="normal" font="default" size="100%">Metastasis</style></keyword><keyword><style  face="normal" font="default" size="100%">Ovarian Cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Proliferation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">1114-1123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Ovarian cancer is a major cause of cancer-related death among women. The growth, persistence, and cancer metastasis are causes of poor prognosis and high mortality rate. Honokiol and magnolol are derivative compounds extracted from the root and stem bark of &lt;em&gt;Magnolia officinalis&lt;/em&gt;. Many studies have reported that honokiol and magnolol have anti-tumour effects on various types of cancer. The present study investigates the anti-tumour effect of these compounds on human ovarian cancer. &lt;strong&gt;Methods:&lt;/strong&gt; Ovarian cancer cell lines, SKOV3 and ES-2 cells were tested with honokiol and magnolol to determine their responses including the cytotoxicity, cell proliferation, induction of cell apoptosis and metastasis ability. &lt;strong&gt;Result:&lt;/strong&gt; The results indicate that low concentrations of honokiol and magnolol suppressed the proliferation of ovarian cancer cells through induction of cell cycle arrest at G0/G1 and down-regulation of the cyclin D1 protein. These compounds also exhibited an anti-metastatic ability mediated by inhibiting migration, adhesion, and MMP activities. Additionally, high concentrations of honokiol and magnolol could activate cell death associated with the apoptosis signalling pathway, either along an intrinsic or extrinsic pathway. &lt;strong&gt;Conclusion: &lt;/strong&gt;The data provides evidence that honokiol and magnolol have potential anti-tumour properties and minimal toxicity on normal cells, and could therefore be applied in the treatment of ovarian cancer.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1114</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Worawat Songjang&lt;sup&gt;1&lt;/sup&gt; and Arunya Jiraviriyakul&lt;sup&gt;2&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Biomedical Science Graduated School, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Medical technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, THAILAND.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Balaji Kyathegowdanadoddi Srinivas</style></author><author><style face="normal" font="default" size="100%">Madhu Chakkere Shivamadhu</style></author><author><style face="normal" font="default" size="100%">Preethi Saligrama Devegowda</style></author><author><style face="normal" font="default" size="100%">Gurukar Mathew</style></author><author><style face="normal" font="default" size="100%">Theethagounder Tamizhmani</style></author><author><style face="normal" font="default" size="100%">Senthilkumar Gnanavadevel Prabhakaran</style></author><author><style face="normal" font="default" size="100%">Shankar Jayarama</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Screening and Evaluation of Lectin and Anti-Cancer Activity from the Phloem Exudate/Sap of the Indian Dietary Ethnomedicinal Plants</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Angiogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">EAC</style></keyword><keyword><style  face="normal" font="default" size="100%">Haemagglutination</style></keyword><keyword><style  face="normal" font="default" size="100%">Lectin</style></keyword><keyword><style  face="normal" font="default" size="100%">VEGF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">570-578</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; Lectins are extremely significant biomolecules to study several biological progressions. In this present investigation, we are screening the crude phloem exudate/ sap sample from different ethnomedicinal plants were evaluated for lectin and anticancer activity. &lt;strong&gt;Methods:&lt;/strong&gt; The lectin activity of crude phloem exudate/sap samples were confirmed by haemgglutination assay and anticancer activity by using trypan blue, MTT and in-ovo CAM angiogenic assay. The tumor cell nuclei resulting in Giemsa stain, AO/EtBr stain, DNA Fragmentation and Caspase- 3 inhibitor assay. &lt;strong&gt;Results:&lt;/strong&gt; Our experimental data show that the phloem exudate/sap sample S2 (Musa Acuminata), sample S4 (Euphorbia Geniculate) exerting the potent lectin activity, sample S5 exerting very low lectin activity against the trypsinized rabbit erythrocytes and decreases the cell viability in EAC cells in-vitro. Sample S2, S4 and S5 exerts significant cytotoxic effect against the various human cancer cell lines and regressed the neovasculature (development of new blood vessels) in the developing CAM embryos when compared to the other crude samples. The apoptotic inducing activity of crude phloem exudate/sap samples was revealed by DNA fragmentation assay, caspase-3 inhibitor assay and cellular morphology were studied by fluorescence staining methods. &lt;strong&gt;Conclusion:&lt;/strong&gt; This study reports that some of the isolated crude phloem exudate/sap samples show potent lectin activity and anti-cancer activity in different human cancer cell lines. The further additional experiment needs to purify and characterize the bioactive lectin components from the potent sample which is responsible for pro-apoptotic, anti-angiogenic activity and mechanism involved.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">570</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Balaji Kyathegowdanadoddi Srinivas&lt;sup&gt;1&lt;/sup&gt;, Madhu Chakkere Shivamadhu&lt;sup&gt;1,2&lt;/sup&gt;, Preethi Saligrama Devegowda&lt;sup&gt;3&lt;/sup&gt;, Gurukar Mathew&lt;sup&gt;4&lt;/sup&gt;, Theethagounder Tamizhmani&lt;sup&gt;5&lt;/sup&gt;, Senthilkumar Gnanavadevel Prabhakaran&lt;sup&gt;5&lt;/sup&gt;, Shankar Jayarama&lt;sup&gt;1&lt;/sup&gt;,*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Post-Graduation Department of Biotechnology, Teresian College, Siddhartha Nagara, Mysore, Karnataka 570011, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biochemistry, Yuvaraja’s College, University of Mysore, Mysuru, Karnataka 570005, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Studies in Biotechnology, University of Mysore, Mysuru, Karnataka 570006, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Botany, Bharathi College, Affiliated University of Mysore, Bharathi Nagara, KM Doddi, Mandya, Karnataka 571422, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Pharmacy, Bharathi College of Pharmacy, Bharathi Nagara, KM Doddi, Mandya, Karnataka 571422, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Swadesh Sarkar</style></author><author><style face="normal" font="default" size="100%">Priya K Gopal</style></author><author><style face="normal" font="default" size="100%">Santanu Paul</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Andrographolide Induced Apoptosis in NALM-6 Cells Mediated Through the Cell Cycle Arrest and Nuclear Fragmentation</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacog Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Andrographis paniculata</style></keyword><keyword><style  face="normal" font="default" size="100%">Andrographolide</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell cycle</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Leukemia</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January-2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/466</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">210-214</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;&lt;em&gt;Andrographis paniculata&lt;/em&gt; is an herb widely cultivated in South and Southeastern Asia. It has been traditionally used to treat infections and other Physiological disorders for several hundreds. We investigated the anti-leukemic potential of Andrographolide (AGP) isolated from the leaves of this plant against an array of cancer cells to investigate its most efficacies in a particular cancer type. &lt;strong&gt;Methods:&lt;/strong&gt; AGP was isolated from &lt;em&gt;Andrographis paniculata&lt;/em&gt; leaves by using column chromatography. The structure was further determined by LC-MS, 1H NMR and 13C NMR. AGP was initially tested against four different cancer cell lines, namely NALM-6 (pre B-ALL), K562 (CML), A549 (lung carcinoma) and MCF-7 (breast carcinoma) using MTT assay at different time points and different concentrations. The effect of the isolated biomolecule was also investigated in inducing apoptosis through the study of cell cycle progression using flow cytometry by PI staining and nuclear fragmentation pattern by DAPI staining and fluorescence microscopy.&lt;strong&gt; Results:&lt;/strong&gt; the spectral analysis of the isolated bio-molecule assured that the compound was AGP. MTT assay data indicated that AGP was most potent to induce cytotoxicity in NALM-6 cells. Further investigation revealed that it effectively induced apoptosis by arresting cell cycle progression and increased the nuclear break down in NALM- 6 leukemic cells. &lt;strong&gt;Conclusion:&lt;/strong&gt; Our study efficiently demonstrated that the AGP isolated from &lt;em&gt;Andrographis paniculata&lt;/em&gt; induced apoptosis in NALM-6 cells, which could be used in the therapeutic intervention of leukemia in the future.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">210                         </style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Swadesh Sarkar&lt;sup&gt;1,2&lt;/sup&gt;, Priya K Gopal&lt;sup&gt;2&lt;/sup&gt;, Santanu Paul&lt;sup&gt;2* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Botany, Chandernagore College, Chandernagore, Hoogly- 712136, West Bengal, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Laboratory of Cell and Molecular Biology, Department of Botany, 35 Ballugunge Circular Road, University of Calcutta, Kolkata- 700019, West Bengal, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">BiYun Gu</style></author><author><style face="normal" font="default" size="100%">Joseph Shalom</style></author><author><style face="normal" font="default" size="100%">Ian E. Cock</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anti-Proliferative Properties of Terminalia sericea Burch. Ex Dc Leaf Extracts Against Caco2 and HeLa Cancer Cell Lines</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant Capacity</style></keyword><keyword><style  face="normal" font="default" size="100%">Antiproliferative Activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Combretaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">DPPH</style></keyword><keyword><style  face="normal" font="default" size="100%">Silver Cluster Leaf</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/499</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">408-415</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Terminalia&lt;/em&gt; spp. are characterised by their high levels of antioxidant phytochemicals and several species have anticancer activity. This study examines the anti-proliferative activity of &lt;em&gt;T. sericea&lt;/em&gt; leaf extracts against Caco2 and HeLa carcinoma cell proliferation. &lt;strong&gt;Methods:&lt;/strong&gt; Solvent extracts were prepared from &lt;em&gt;T. sericea&lt;/em&gt; leaves and their antioxidant capacities were determined by the DPPH free radical scavenging assay. Anti-proliferative activities against Caco2 and HeLa cancer cells were determined by an MTS based cell proliferation assay. Toxicity was determined using the &lt;em&gt;Artemia franciscana&lt;/em&gt; nauplii bioassay. &lt;strong&gt;Results:&lt;/strong&gt; The methanolic and aqueous &lt;em&gt;T. sericea&lt;/em&gt; leaf extracts displayed high antioxidant capacities (equivalent to 150 and 340 mg of ascorbic acid per gram of plant material extracted respectively). In contrast, the ethyl acetate, chloroform and hexane extracts had relatively low antioxidant contents (&amp;le;5 mg of ascorbic acid equivalents per gram of plant material extracted). The antioxidant contents of the &lt;em&gt;T. sericea&lt;/em&gt; leaf extracts correlated with the ability of the extracts to inhibit proliferation of Caco2 and HeLa cancer cell lines. The high antioxidant methanolic and aqueous extracts were potent inhibitors of cell proliferation, with IC&lt;sub&gt;50&lt;/sub&gt; values 120-1400 &amp;mu;g/mL. The aqueous &lt;em&gt;T. sericea&lt;/em&gt; leaf extract was particularly effective, with IC&lt;sub&gt;50&lt;/sub&gt; values of 528 and 120 &amp;mu;g/mL against Caco2 and HeLa cells respectively. The methanolic extract also displayed good, albeit substantially less potent, antiproliferative activity against HeLa cells, with an IC&lt;sub&gt;50&lt;/sub&gt; of 1358 &amp;mu;g/mL. In contrast, the lower antioxidant content extracts generally did not inhibit cancer cell proliferation. Cell imaging studies detected morphological features consistent with apoptosis in Caco2 cells exposed to sub-lethal concentrations of the methanolic and aqueous T. sericea leaf extracts, indicating that these extracts are functioning by cytotoxic mechanisms. The aqueous &lt;em&gt;T. sericea&lt;/em&gt; leaf extract displayed low to moderate toxicity in the &lt;em&gt;Artemia franciscana&lt;/em&gt; bioassay, with an LC&lt;sub&gt;50&lt;/sub&gt; value of 737 &amp;mu;g/mL. All other extracts were nontoxic. &lt;strong&gt;Conclusion:&lt;/strong&gt; The antiproliferative activity and low toxicity of the &lt;em&gt;T. sericea &lt;/em&gt;methanolic and aqueous leaf extracts extracts against HeLa and Caco2 cancer cell lines indicates their potential in the treatment and prevention of some cancers.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">408</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;BiYun Gu&lt;sup&gt;1,2&lt;/sup&gt;, Joseph Shalom&lt;sup&gt;1,3&lt;/sup&gt;, Ian E. Cock&lt;sup&gt;1,3* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;School of Natural Sciences, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland 4111, AUSTRALIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, CHINA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland 4111, AUSTRALIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Aswathy Jayasree Madanakumar</style></author><author><style face="normal" font="default" size="100%">Bosco Lawarence</style></author><author><style face="normal" font="default" size="100%">Manoj GS</style></author><author><style face="normal" font="default" size="100%">Murugan Kumaraswamy</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Purified Anthocyanin from in vitro Culture of Bridelia retusa (L.) Spreng. Capable of Inhibiting the Growth of Human Oral Squamous Cell Carcinoma Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anthocyanin</style></keyword><keyword><style  face="normal" font="default" size="100%">Anti-metastatic potential</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Bridelia retusa</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell suspension</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro culture</style></keyword><keyword><style  face="normal" font="default" size="100%">Purification</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/524</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">559-566</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;The present study aims &lt;em&gt;in vitro&lt;/em&gt; cell suspension culture of &lt;em&gt;Bridelia retusa&lt;/em&gt;, isolation of anthocyanin, purification, fractionation and its anti-metastatic potential against oral squamous carcinoma cells. Experimental results reveal that 2, 4-D either alone or in combination with kinetin supplemented in MS medium showed significant initiation of callus from leaf explants than stem. Growth hormones, pH, light, and carbon source influence anthocyanin synthesis. Maximum callus induction was noticed with 2.5 mg/L N6-benzyladenine (BA) + 2 mg/L 2, 4-dichlorophenoxyacetic acid (2, 4-D) (98.9%). Fresh and dry weight of the calli were i.e., 1.9 &amp;plusmn; 0.04 and 0.45 &amp;plusmn; 0. 03 g respectively. Optimal response was seen with light on MS medium contain 4% glucose + 2.5 mg/L BA and 2 mg/L 2, 4-D at pH 3.5 yielded 2.8 mg /g of anthocyanins. Suspension culture medium fortified with 2, 4-D (2.5 mg/L) + BA (2 mg/L) at pH 5.0 induced anthocyanin production at pH 4.4 &amp;ndash; 4.6. HCl-ethanol extraction for 90 min yielded the maximum anthocyanin content. Fractionation of anthocyanin using HPLC coupled with mass spectrometry revealed 07 fractions such as acylated cyanidins, two peonidins, cyanidin 3-p-coumaroyl and feruloyl diglucoside-5-glucosides. In the search of novel therapeutic drugs against cancer, cytotoxicity effect of &lt;em&gt;B.retusa&lt;/em&gt; anthocyanin extracts on human oral squamous cell carcinoma (SCC4, SCC9 and SCC25) cells using cell adhesion and cell viability assay was carried. The morphological alterations in SCCs cells after treatment with &lt;em&gt;B.retusa&lt;/em&gt; anthocyanin includes nuclear condensation, fragmentation and apoptotic cells as revealed by Hoechst stain. Flow cytometry showed arresting of SCC25 cells mostly in the G0/G1 and S-G2/M stages with a concomitant up regulation of sub-G1 fraction, indicating cell death by apoptosis. Apoptosis was further substantiated by the activation of caspase-3 expression in the SCC25 cells treated with &lt;em&gt;B.retusa&lt;/em&gt; anthocyanin. Thus, it is possible to suggest that &lt;em&gt;B.retusa&lt;/em&gt; anthocyanin cause apoptosis of SCCs and warrant further investigation using animal models.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">559</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Aswathy Jayasree Madanakumar&lt;sup&gt;1&lt;/sup&gt;, Bosco Lawarence&lt;sup&gt;2&lt;/sup&gt;, Manoj GS&lt;sup&gt;3&lt;/sup&gt;,Murugan Kumaraswamy&lt;sup&gt;1*&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Botany, Plant Biochemistry and Molecular biology Laboratory, University College, Thiruvananthapuram, Kerala- 695 034, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Botany and Biotechnology, Govt. Arts College, Trivandrum-14, Kerala, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Botany, Nilamel NSS College, Kerala, INDIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Swadesh Sarkar</style></author><author><style face="normal" font="default" size="100%">Santanu Paul</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Triptolide Mediated Amelioration of Breast Cancer via Modulation of Molecular Pathways</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Breast cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular pathway.</style></keyword><keyword><style  face="normal" font="default" size="100%">Tripterigium wilfordii</style></keyword><keyword><style  face="normal" font="default" size="100%">Triptolide</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/184</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">838-845</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;Triptolide is the main bioactive molecule isolated from a root extract of &lt;em&gt;Tripterigium wilfordii&lt;/em&gt; Hook F. of Celastraceae family. Chemically, it is a diterpenoid triepoxide molecule and its chemical formula is C&lt;sub&gt;20&lt;/sub&gt;H&lt;sub&gt;24&lt;/sub&gt;O&lt;sub&gt;6&lt;/sub&gt;. Its five-membered unsaturated lactone ring (D-ring) is crucial for anti-tumor potential and carbonyl group at C-18 position is essential to exert important influence on the interaction between triptolide and the targeted protein(s). It is bio-synthesized from deoxy-D-xylullose-5-phosphate (DOXP) pathway in the cell. Triptolide can induce apoptosis in a number of breast cancer cells by up-regulating different pro-apoptotic and down-regulating different anti-apoptotic molecules. &lt;em&gt;In vitro&lt;/em&gt; experiments indicate that it can down regulate several cell cycle related genes and induces S-phase cell cycle arrest. Triptolide treatment can also modulate the expression of different cell signaling molecules, e.g. ERK, NF-&amp;kappa;B, FAK, VEGF, &amp;beta;-catenin, AKT etc. &lt;em&gt;In vivo&lt;/em&gt; experiments indicate that triptolide can effectively reduce breast tumor growth in the mouse model. Apart from the single drug treatment, triptolide can effectively be applied in combination therapy. Application of Triptolide with other chemotherapeutic drugs, very efficiently check the proliferation of tumor cells which reduces the effective concentration of the commercially available drugs thus reducing their toxic sideeffects. Although triptolide is very effective against a number of diseases, its higher degree of multi-organ toxicity limits its use of further clinical trial. Therefore, to reduce the toxic effects, a number of strategies have been developed which increase its water solubility and at the same time decrease the toxic effect. In this review article, we have addressed how triptolide participates in the antitumor processes in breast cancer cells.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">838</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Swadesh Sarkar&lt;sup&gt;1,2&lt;/sup&gt;, Santanu Paul&lt;sup&gt;2* &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Botany, Chandernagore College, Chandernagore, Hoogly- 712136, West Bengal, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Laboratory of Cell and Molecular Biology, Department of Botany, 35 Ballugunge Circular Road, University of Calcutta, Kolkata- 700019, West Bengal, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>