<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mfundisi Nhlapo</style></author><author><style face="normal" font="default" size="100%">Brian Ngobeni</style></author><author><style face="normal" font="default" size="100%">Idah Manduna</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A Review: Medicinal Uses, Phytochemistry and Pharmacological Properties of Plants from the Hermannia Genus</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bioactive compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">drug development</style></keyword><keyword><style  face="normal" font="default" size="100%">Hermannia</style></keyword><keyword><style  face="normal" font="default" size="100%">Pharmacological activities</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword><keyword><style  face="normal" font="default" size="100%">Traditional medicine</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">384-393</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Medicinal plants play a pivotal role in treating illnesses and modern medicines are still being derived from plants. Hermannia genus is a significant traditional herbal medicine. This review evaluates the medicinal uses, phytochemistry and pharmacological properties of plants from the genus Hermannia genus based on available research. &lt;strong&gt;Methods:&lt;/strong&gt; Studies accessed from online research databases were systematically selected and analysed to construct a comprehensive review of the medicinal uses, phytochemistry and pharmacological properties of plants from the genus. &lt;strong&gt;Results: &lt;/strong&gt;Hermannia species are used in traditional medicine to treat or manage; respiratory conditions, gastrointestinal issues, skin conditions, sexually transmitted infections, and diabetes. Scientific findings also discovered promising pharmacological activities within members of the genus such as antimicrobial, anti-inflammatory, antioxidant, antidiabetic and anticancer activities. To date, over 30 types of secondary metabolites have been identified from the genus, including the 2 pure compounds that were isolated and tested for pharmacological activities. Further research must prioritize other unexplored species of the genus and efficacy and mechanism of action studies on isolated compounds. &lt;strong&gt;Conclusion: &lt;/strong&gt;The genus Hermannia is important in the treatment of diseases of high public health concern. The pharmacological studies and presence of secondary metabolites and bioactive compounds further validates the traditional uses of the genus. Therefore, the findings suggest that the genus has species that may serve as candidates for novel drug discovery for the treatment of various illnesses. Efficacy and mechanism of action studies still need to be conducted on isolated compounds and other unexplored species of the genus.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Review Article</style></work-type><section><style face="normal" font="default" size="100%">384</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Mfundisi Nhlapo&lt;sup&gt;1&lt;/sup&gt;, Brian Ngobeni&lt;sup&gt;2*&lt;/sup&gt;, Idah Manduna&lt;sup&gt;3&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Health Sciences, Central University of Technology, Free State, SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Clinical Sciences, Central University of Technology, Free State SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Centre for Applied Food Sustainability and Biotechnology, Faculty of Health and Environmental Sciences, Central University of Technology, Free State, SOUTH AFRICA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ololade Zacchaeus S</style></author><author><style face="normal" font="default" size="100%">Anuoluwa Iyadunni A</style></author><author><style face="normal" font="default" size="100%">Adeyemi Adewale F</style></author><author><style face="normal" font="default" size="100%">Uyaboerigha Daubotei I</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Synergistic Efficacy of Phytochemical, Antioxidant and Bactericidal Properties of the Aerial Essential Oil of Laggera crispata</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Aerial essential oil</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Asteraceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Laggera crispata</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">1304-1311</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;This study was undertaken to provide more scientific information about the phytochemical composition of Laggera crispata known for its medicinal uses. Essential oil was isolated by hydro-distillation, analysed using GC-MS, UV-Visible Spectrophotometer and other established biochemical assays were used for the study. The GC-MS analysis of the aerial essential oil of&lt;em&gt; L. crispata&lt;/em&gt; showed the presence of 35 medicinal organic compounds making up 84.4% of the oil. The most abundant component was a phenolic compound called 2-tert-Butyl&lt;sup&gt;-1&lt;/sup&gt;,4-dimethoxybenzene (54.5%). The other major terpenoids present in the oil were α-humulene (6.9%) and (+)-sabinene (5.9%). The TPC, TFC, TAA and TAC values of the aerial essential oil of &lt;em&gt;L. crispata &lt;/em&gt;were 172.75±0.00 μgmg&lt;sup&gt;-1&lt;/sup&gt; GAE, 48.69±0.00 μgmg&lt;sup&gt;-1&lt;/sup&gt; QE, 61.85±0.00 μgmg&lt;sup&gt;-1&lt;/sup&gt; AAE and 726.92±0.00 μgmg&lt;sup&gt;-1&lt;/sup&gt; AAE respectively. DPPH IC&lt;sub&gt;50 &lt;/sub&gt;and AAI values of the essential oil were 1.5 μgml&lt;sup&gt;-1&lt;/sup&gt; and 26.7. The essential oil displayed varying inhibitory activities against Gram-positive and Gram-negative bacteria with zones of inhibition ranging from 08-30 mm. The&lt;em&gt; in vitro&lt;/em&gt; pharmacological activities added scientific support to the use of &lt;em&gt;L. crispata&lt;/em&gt; in alternative and complementary medicine. The essential oil of &lt;em&gt;L. crispata&lt;/em&gt; grown in Nigeria will play beneficial roles in human and animal health and therefore a research on this plant might be of great value in drug industries.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1304</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ololade Zacchaeus S&lt;sup&gt;1,&lt;/sup&gt;*, Anuoluwa Iyadunni A&lt;sup&gt;2&lt;/sup&gt;, Adeyemi Adewale F&lt;sup&gt;1&lt;/sup&gt;, Uyaboerigha Daubotei I&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Chemistry, University of Medical Sciences, Ondo, NIGERIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biological Sciences, University of Medical Sciences, Ondo, NIGERIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Plant Biology and Biotechnology, University of Benin, NIGERIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vasanth MP</style></author><author><style face="normal" font="default" size="100%">KG Purushotham</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Screening of Phytochemical Analysis and In vitro Bioactive of Polyherbal Formulation</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">antioxidant activity</style></keyword><keyword><style  face="normal" font="default" size="100%">FTIR</style></keyword><keyword><style  face="normal" font="default" size="100%">HR-LCMS</style></keyword><keyword><style  face="normal" font="default" size="100%">Phyto chemical</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1525-1533</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Plants have the capability to synthesize various forms of phytochemical compounds as secondary metabolites. &lt;strong&gt;Method: &lt;/strong&gt;In the present investigation phytochemicals such as alkaloids, tannins, glycosides, carbohydrates, reducing sugar, proteins, saponins, flavanoids, phenols, terpenoids and phytosterols were detected in polyherbal formulation &lt;em&gt;A. marmelos, G. glabra and R. centrifolia. &lt;/em&gt;&lt;strong&gt;Results:&lt;/strong&gt; The Total flavonoid, steroids, alkaloids and phenolic content was observed from fruit, root and pettles1.40mg QE/g DE, 12.14mg BE/g DE, 14.40mg AE/g DE and 99.33mg GAE/g DE. FTIR spectrum of the polyherbal sample revealed 5 major peaks at 2919.40 cm&lt;sup&gt;-1&lt;/sup&gt;, 2357.62 cm&lt;sup&gt;-1&lt;/sup&gt;, 1150.56 cm&lt;sup&gt;-1&lt;/sup&gt;, 1076.22 cm&lt;sup&gt;-1&lt;/sup&gt; and 1015.64 cm&lt;sup&gt;-1&lt;/sup&gt;. The antibacterial activity was maximum zone of inhibition (19 mm) was recorded in S. aureus strain and minimum zone of inhibition (5mm) was observed in S.mutans strain. The antioxidant study maximum and minimum scavenging DPPH, NOR, H2O2 and SOD activities (%) of 62.28, 53.68, 39.67 &amp;amp; 43.98 at 5 mg/ml and 39.88, 36.49, 5.9 and 34.94 at 1mg/ml was recorded. The polyherbal sample exhibited significant albumin denaturation, proteinase inhibitory, membrane stabilization and lipid peroxidation inhibitory activities as the maximum inhibition of 46.53%, 36.7%, 51.9% and 64.71% was observed at 500 μg/ml. &lt;strong&gt;Conclusion:&lt;/strong&gt; Hence the present studies indicate good antibacterial, antioxidant and anti-inflammatory activities from the medicinal plants, &lt;em&gt;A. marmelos, G. glabra and R. centrefolia &lt;/em&gt;proves the possibility of its utilization as an additional potent source medicinal uses.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1525</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Vasanth MP, KG Purushotham* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Department of Biotechnology, Dr. M.G.R EDUCATIONAL AND RESEARCH INSTITUTE, Chennai-600095, Tamilnadu, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vanessa de Andrade Royo</style></author><author><style face="normal" font="default" size="100%">Juliana Almeida Rocha</style></author><author><style face="normal" font="default" size="100%">Kamylla Teixeira Santos</style></author><author><style face="normal" font="default" size="100%">Jeane Ferreira Leal Freitas</style></author><author><style face="normal" font="default" size="100%">Clarice Avelar Almeida</style></author><author><style face="normal" font="default" size="100%">Bianca Ribeiro</style></author><author><style face="normal" font="default" size="100%">Elytania Veiga Menezes</style></author><author><style face="normal" font="default" size="100%">Dario Alves de Oliveira</style></author><author><style face="normal" font="default" size="100%">Murilo Malveira Brandão</style></author><author><style face="normal" font="default" size="100%">Afranio Farias de Melo Júnior</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative Studies Between Mauritia flexuosa and Mauritiella armata</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antioxidant activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Arecaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Flavonoids</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemical</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2018</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">32-36</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Mauritia flexuosa&lt;/em&gt; and &lt;em&gt;Mauritiella armata&lt;/em&gt; belong to the family Arecaceae and are widely found in Brazil. &lt;strong&gt;Aim:&lt;/strong&gt; In this work were evaluated: the phytochemical profile of the secretion popularly known as &lt;em&gt;M. flexuosa&lt;/em&gt; wine, antioxidant activity of leaf, root and petiole hydroethanolic extracts of the two species, as well as the quantification of flavonoids and the chromatographic profile by means of high performance liquid chromatography. &lt;strong&gt;Material and Methods:&lt;/strong&gt; The chromatographic profile was determined by high performance liquid chromatography, quantification of flavonoids and antioxidant activity, were performed by spectrophotometric method. &lt;strong&gt;Results:&lt;/strong&gt; Antioxidant activity and presence of flavonoids were observed in the extracts of all the analyzed structures of the two species. The phytochemical profile of the wine evidenced the presence of secondary metabolites reported in other structures of &lt;em&gt;M. flexuosa&lt;/em&gt;. In the chromatographic analysis, it was observed that the extracts evaluated have between three and nine compounds.&lt;strong&gt; Conclusion:&lt;/strong&gt; Further studies should be performed to identify the active compounds in the two species.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">32</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Vanessa de Andrade Royo&lt;sup&gt;1,*&lt;/sup&gt;, Juliana Almeida Rocha&lt;sup&gt;1&lt;/sup&gt;, Kamylla Teixeira Santos&lt;sup&gt;1&lt;/sup&gt;, Jeane Ferreira Leal Freitas&lt;sup&gt;1&lt;/sup&gt;, Clarice Avelar Almeida&lt;sup&gt;1&lt;/sup&gt;, Bianca Ribeiro&lt;sup&gt;1&lt;/sup&gt;, Elytania Veiga Menezes&lt;sup&gt;2&lt;/sup&gt;, Dario Alves de Oliveira&lt;sup&gt;2&lt;/sup&gt;, Murilo Malveira Brandão&lt;sup&gt;2&lt;/sup&gt;, Afranio Farias de Melo Júnior&lt;sup&gt;2 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Laboratory of Natural Products, State University of Montes Claros, Avenida Dr. Ruy Braga, S/N - Vila Mauriceia, 39401-089, Montes Claros - MG, BRAZIL.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Laboratory of Bioporpection and Genetic Resources, State University of Montes Claros, Avenida Dr. Ruy Braga, S/N - Vila Mauriceia, 39401-089, Montes Claros - MG, BRAZIL.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Venkataraghavan Ragunathan</style></author><author><style face="normal" font="default" size="100%">Jayashree Pandurangan</style></author><author><style face="normal" font="default" size="100%">Thiruchelvi Ramakrishnan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Gas Chromatography-Mass spectrometry Analysis of Methanol Extracts from Marine Red Seaweed Gracilaria corticata</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bioactive compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">GC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Gracilaria corticata</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicinal properties</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">547-554</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;The objective of the work is to analyse the methanol extract of marine red macro algae species &lt;em&gt;Gracilaria corticata &lt;/em&gt;using Gas chromatography-Mass spectrometry (GC-MS) to reveal the presence of various secondary metabolites and bioactive compounds present in the algae and study its diverse properties.&lt;strong&gt; Methods: &lt;/strong&gt;&lt;em&gt;Gracilaria corticata&lt;/em&gt; was collected along the shore of Mandapam and was identified and authenticated. The methanol extract of the algae was prepared and analysed using GC-MS Perkin-Elmer, Clarus 680 model to reveal the various bioactive present in the algae. &lt;strong&gt;Results: &lt;/strong&gt;The analysis revealed several bioactive compounds:undecane; 2-decyloxirane (2.023%); Methy n-tridecanoate;n-hexadecanoic acid (74.198%); eicosanoic acid (2.262%); nonanoic acid (2.084%); oleic acid (6.609%); oleic acid (4.156%); pentadecanoic acid (2.176%); bicycle [3.2.1] oct-3-en-2-one,3,8-dihydroxy- 1-1methoxy-7-(7-methoxy-1, 3 benzodioxol-5-yl)-6-methyl-5 (2.901%);N-(5-chloro-2-hydroxyphenyl) dodecanamide (2.048%); and cholesta-8,24-dien-3-ol,4-methyl (1.542%). The bioactive compounds from methanol extract of algae after GC-MS analysis and their essential medicinal properties were studied in this research work. &lt;strong&gt;Conclusion:&lt;/strong&gt; &lt;em&gt;Gracilaria corticata &lt;/em&gt;has potential against bacteria, fungi, free radical scavenging, etc and can used in the drug discovery and development sector.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">547</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Venkataraghavan Ragunathan, Jayashree Pandurangan, Thiruchelvi Ramakrishnan* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies, Chennai- 600117, Tamil Nadu, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rinto Muhammad Nur</style></author><author><style face="normal" font="default" size="100%">Laurentius Hartanto Nugroho</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic Activities of Fractions from Dioscorea bulbifera L. Chloroform and Methanol Extracts on T47D Breast Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">D. bulbifera</style></keyword><keyword><style  face="normal" font="default" size="100%">MTT Assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword><keyword><style  face="normal" font="default" size="100%">T47D</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/362</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">33-38</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; To elucidate cytotoxic activity of fractions from chloroform and methanol extracts of &lt;em&gt;D. bulbifera &lt;/em&gt;organs on T47D breast cancer cells. &lt;strong&gt;Method:&lt;/strong&gt; The vegetative organs of &lt;em&gt;D. bulbifera&lt;/em&gt; were extracted gradually using chloroform and methanol. Cytotoxicity tested on T47D cells using MTT Assay. The most toxic extract was fractioned by vacuum liquid chromatography (VLC) followed by thin layer chromatography (TLC). The extract and fractions potential were tested on the Vero cells using the same method as cancer cells. The most toxic fraction was analyzed using TLC followed by the application of various spray reagents for the identification of active compound. &lt;strong&gt;Results:&lt;/strong&gt; The chloroform extract of the &lt;em&gt;D. bulbifera &lt;/em&gt;leaves was the highest cytotoxic on T47D cells (IC&lt;sub&gt;50&lt;/sub&gt; 115.63&amp;plusmn;86.01 &amp;mu;g/mL). Moreover, the cytotoxicity test on the combined fractions of leaves chloroform extract showed that fraction 5 (F5) and fraction 6 (F6) were the most toxic fractions compared to those of other fractions. The IC&lt;sub&gt;50&lt;/sub&gt; of both fractions were 14.55&amp;plusmn;8.62 and 7.12&amp;plusmn;4.43 &amp;mu;g/mL respectively. However, Its were very weak compared to those of cancer medicine (Doxorubicin) with the IC&lt;sub&gt;50&lt;/sub&gt; was 0.04&amp;plusmn;0.02 &amp;mu;g/mL. Potential fractions were not toxic against Vero cells with IS&amp;gt;10. The active compounds in those fractions were alkaloid and terpenoid. &lt;strong&gt;Conclusion:&lt;/strong&gt; Chloroform extract of the &lt;em&gt;D. bulbifera&lt;/em&gt; leaves had the highest cytotoxic effect on T47D cells. Potential fractions were not toxic against Vero cells. The active compounds in those fractions were alkaloid and terpenoid.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">33</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Rinto Muhammad Nur&lt;sup&gt;1&lt;/sup&gt;, Laurentius Hartanto Nugroho&lt;sup&gt;2&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Fisheries and Marine Science, Universitas Pasifik Morotai, Jln. Siswa Darame, Kec. Morotae Selatan, Kab. Morotae, Maluku Utara, INDONESIA.&lt;/p&gt;
&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan, Sekip Utara, Yogyakarta, INDONESIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Imane Chamkhi</style></author><author><style face="normal" font="default" size="100%">Laila Sbabou</style></author><author><style face="normal" font="default" size="100%">Jamal Aurag</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Endophytic Fungi Isolated from Crocus sativus L. (Saffron) as a Source of Bioactive Secondary Metabolites</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antibacterial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">antioxidant activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Crocus sativus L.</style></keyword><keyword><style  face="normal" font="default" size="100%">Endophytic fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">ITS rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary metabolites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2018</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">1143-1148</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Endophytic fungi are becoming an important source of new natural bioactive products. Many interesting endophytic fungi have been isolated from traditional medicinal plants. &lt;em&gt;Crocus sativus&lt;/em&gt; L. (saffron) is one of the most expensive and rarest spices in the world, used as a dye, aroma and for medicinal purposes. This study reports on the molecular characterization of endophytic fungi isolated from roots of saffron plants growing in Taliouine- Morocco, and the examination of the antibacterial and antioxidant activities of secondary metabolites extracted from these endophytes. &lt;strong&gt;Methods and Material:&lt;/strong&gt; The fungi were isolated from sterilized saffron root fragments and identified using internal transcribed spacer (ITS) rRNA gene sequence analysis. Three endophytes were fermented and extracted with ethyl acetate (EtOAc) and chloroform (CHCl3). The antibacterial activity of fungi extracts was evaluated using the Agar Diffusion Method against six strains,&lt;em&gt; Bacillus&lt;/em&gt; &lt;em&gt;sp&lt;/em&gt;., &lt;em&gt;Stenotrophomonas sp., Pseudomonas putida, Pantoea sp., Luteibacter sp.&lt;/em&gt; and &lt;em&gt;Escherichia coli&lt;/em&gt;. The antioxidant activity was tested by using the &amp;alpha;,&amp;alpha;-diphenyl-&amp;beta;-picrylhydrazyl (DPPH) analysis and &amp;beta;-Carotene bleaching test (BCBT) methods. &lt;strong&gt;Results:&lt;/strong&gt; Sixty fungal isolates were recovered and purified from saffron roots. ITS rDNA sequences were 99-100% identical to three different species: Rhizopus oryzae, Aspergillus fumigatiaffinis and &lt;em&gt;Aspergillus niger. &lt;/em&gt;Both &lt;em&gt;Rhizopus oryzae&lt;/em&gt; extracts had high antibacterial activity against most of the bacteria tested, while &lt;em&gt;Aspergillus niger and Rhizopus oryzae&lt;/em&gt; extracts showed an antioxidant capacity using DPPH and BCBT methods, respectively.&lt;strong&gt; Conclusion:&lt;/strong&gt; The fungal endophytes inhabiting saffron roots could be a potential source of natural plant bioactive secondary metabolites especially for antibacterial and antioxidant purposes.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1143</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Imane Chamkhi*, Laila Sbabou, Jamal Aurag&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnology, Biodiversity and Environment. Faculty of Sciences, Mohammed V University in Rabat, MOROCCO.&lt;/p&gt;</style></auth-address></record></records></xml>