<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Renny Aditya</style></author><author><style face="normal" font="default" size="100%">Budi Santoso</style></author><author><style face="normal" font="default" size="100%">Widjiati</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Alteration of IL-6, BMP-15 and GDF-9 Levels on PCOS Rat Models  After Treated with Syzygium Polyanthum (Wight) Walp Leaves Extract</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BMP-15</style></keyword><keyword><style  face="normal" font="default" size="100%">GDF-9</style></keyword><keyword><style  face="normal" font="default" size="100%">IL-6</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">1084-1090</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Polycystic ovary syndrome (PCOS) is a global health concern for women in reproductive age women. Numerous studies have been reported an association between chronic inflammation and alteration of cytokine in women with PCOS. &lt;em&gt;Syzygium polyanthum&lt;/em&gt; (&lt;em&gt;S. polyanthum&lt;/em&gt;) contains antioxidants and has antiinflammation activity. &lt;strong&gt;Objectives:&lt;/strong&gt; This study aims to measure the alteration of IL-6, BMP-15, and GDF-9 in rat PCOS model after treated with &lt;em&gt;S. polyanthum&lt;/em&gt; leaves extract.&lt;strong&gt; Materials and Methods: &lt;/strong&gt;The female Wistar rats were divided into five groups (n = 5), K0 (normal control), K1 (PCOS group), and three treatment groups which received three different doses of&lt;em&gt; S. polyanthum &lt;/em&gt;leaves extract. The treatment group consisted of PCOS rat models with &lt;em&gt;S. polyanthum&lt;/em&gt; leaves extract supplementation of 150 mg/KgBW (P1), 300 mg/KgBW (P2), and 450 mg/KgBW (P3). &lt;strong&gt;Results:&lt;/strong&gt; IL-6 expression was highest in K1 (4,690 ± 0.099) and lowest in the P3 treatment, namely (2,370 ± 0.105). The expression of BMP-15 and GDF-9 was lowest at K1 (2.554 ± 0.04; 4.502 ± 0.050) and highest at P3, namely (2.265 ± 0.072; 4.736±0.074). &lt;strong&gt;Conclusion: &lt;/strong&gt;&lt;em&gt;S. polyanthum &lt;/em&gt;leaves extract was significantly effective in decreasing IL-6 expressions, as well as a significant increase in BMP-15 and GDF-9 expressions in the PCOS rat model.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1084</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Renny Aditya&lt;sup&gt;1,2&lt;/sup&gt;, Budi Santoso&lt;sup&gt;3,&lt;/sup&gt;*, Widjiati&lt;sup&gt;4&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Renny Aditya</style></author><author><style face="normal" font="default" size="100%">Budi Santoso</style></author><author><style face="normal" font="default" size="100%">Widjiati</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The Effect of Syzygium Polyanthum (Wight) Walp. Extract on Glutathione, Tumour Necrosis Factor-Alpha, Spdl1, And Degraff Follicles Expression in PCOS Rat Models</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DeGraff follicle count</style></keyword><keyword><style  face="normal" font="default" size="100%">GSH</style></keyword><keyword><style  face="normal" font="default" size="100%">sPDL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">TNF-.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">791-800</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Polycystic ovary syndrome (PCOS) is an endocrine and reproductive disorder affecting 5% to 13% of women of reproductive age. Approximately 80% of women with PCOS underwent infertility cases. Many studies have shown an association between PCOS and low-grade inflammation. Syzygium polyanthum (&lt;em&gt;S. polyanthum&lt;/em&gt;) contains antioxidants and has antiinflammation activity. &lt;strong&gt;Objectives: &lt;/strong&gt;This study aims to investigate the effectiveness of &lt;em&gt;S. polyanthum&lt;/em&gt; leaf on glutathione, tumour necrosis factoralpha (TNF-α), sPDL1, and DeGraff follicle expression in PCOS rat models.&lt;strong&gt; Materials and Methods: &lt;/strong&gt;The female Wistar rats were divided into five groups (n = 5), K0 (normal control), K1 (PCOS group), and three treatment groups which received three different doses of &lt;em&gt;S. polyanthum&lt;/em&gt; extract. The treatment group consisted of PCOS rat models with &lt;em&gt;S. polyanthum&lt;/em&gt; extract supplementation of 150 mg/KgBW (P1), 300 mg/KgBW (P2), and 450 mg/KgBW (P3). &lt;strong&gt;Results:&lt;/strong&gt; &lt;em&gt;S. polyanthum&lt;/em&gt; leaf extract significantly decreased TNF-α expression in the treatment group. The highest TNF-α, GSH, and sPDL1 concentration was found in the P3 group (3.83±0.123, 273.12±12.4 ng/ml, and 3.25 ng/ml, respectively). Moreover, the number of follicles was statistically significant in the P1, P2, and P3 groups (2.0, 3.0, and 3.50, respectively). The number of follicles in the P1, P2, and P3 groups was higher than in the positive control; however, it was lower in the negative control (K0) (p&amp;lt;0.05). &lt;strong&gt;Conclusion:&lt;/strong&gt; &lt;em&gt;S. polyanthum &lt;/em&gt;extract was significantly effective in decreasing TNF-α expression, as well as a significant increase GSH, sPDL1 and DeGraff foliclle count in PCOS rat model.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">791</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Renny Aditya&lt;sup&gt;1&lt;/sup&gt;, Budi Santoso&lt;sup&gt;2,*&lt;/sup&gt;, Widjiati&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA;&lt;span style=&quot;font-size:10.8333px&quot;&gt; &lt;/span&gt;Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Erlena Nor Asmira Abdul Rahim</style></author><author><style face="normal" font="default" size="100%">Muhammad Nor Omar</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antihypertensive Assay-Guided Fractionation of Syzygium polyanthum Leaves and Phenolics Profile Analysis Using LCQTOF/ MS</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antihypertensive</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioassay-guided</style></keyword><keyword><style  face="normal" font="default" size="100%">LCMS</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">Total phenol content (TPC)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1670-1692</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Syzygium polyanthum&lt;/em&gt; leaves extract that contains gallic acid as the major phenolic compound has shown significant antihypertensive effect, however the amount of gallic acid was inversely-related with magnitude of this effect. This study aimed to conduct bioassay-guided fractionation of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves with gallic acid as a reference compound, and to screen for other possible compounds responsible for the antihypertensive effect. &lt;strong&gt;Methods: &lt;/strong&gt;&lt;em&gt;S. polyanthum &lt;/em&gt;leaves were extracted using n-hexane, ethyl acetate, methanol, and water. The most active crude extract was fractionated using column chromatography and analyzed for total phenolic content (TPC) (n=3). Crude extracts and the derived fractions were intravenously administered into pentobarbital-anaesthetized Spontaneously Hypertensive rats (n=5) for recording of blood pressure parameters. Liquid Chromatography-Quadruple Time-Off-Flight/Mass Spectrometry was used for determination of chemical composition. One-way and two-way ANOVA were used for statistical analysis using GraphPad® PRISM Version 6. &lt;strong&gt;Results: &lt;/strong&gt;Fractionation of aqueous &lt;em&gt;S. polyanthum&lt;/em&gt; leaves extract (ASP) afforded nine fractions, later combined into three fractions (F1ASP, F2ASP, and F3ASP) based on the thinlayer chromatography profiles. ASP has the highest TPC while F2ASP has the lowest TPC. All fractions exhibited significant antihypertensive property, but F2ASP was the most active fraction. Few phenolics with related antihypertensive effects such as 1-galloyl glucose (a gallic acid-derivative majorly found in F2ASP and F3ASP), and other compounds such as polydatin, sesamol, brazilin, eugenol, ellagic acid, kukoamine A, and cyclocurcumin were found across all active fractions. &lt;strong&gt;Conclusion: &lt;/strong&gt;These phenolics may partly contribute to the antihypertensive effect of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves, thus further isolation study is recommended.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">167</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Azlini Ismail&lt;sup&gt;1,&lt;/sup&gt;*, Erlena Nor Asmira Abdul Rahim&lt;sup&gt;2&lt;/sup&gt;, Muhammad Nor Omar&lt;sup&gt;2&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kuliyyah of Dentistry, International Islamic University Malaysia, Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biotechnology, Kuliyyah of Science, International Islamic University Malaysia, Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, MALAYSIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ika Rahayu</style></author><author><style face="normal" font="default" size="100%">Pamela Hendra Heng</style></author><author><style face="normal" font="default" size="100%">Kris H Timotius</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro Antioxidant Properties and α-Glucosidase Inhibition of Combined Leaf Infusions from Psidium guajava L., Syzygium polyanthum L., and Annona muricata L.</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anonna muricata</style></keyword><keyword><style  face="normal" font="default" size="100%">Leaf infusion</style></keyword><keyword><style  face="normal" font="default" size="100%">Psidium guajava</style></keyword><keyword><style  face="normal" font="default" size="100%">Synergism</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">α-glucosidase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">1269-1277</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Guava (&lt;em&gt;P. guajava&lt;/em&gt;), bay (&lt;em&gt;S. polyanthum&lt;/em&gt;), and soursop (&lt;em&gt;A. muricata&lt;/em&gt;) known as natural medicine. Limited report is available on their antioxidant and α-glucosidase inhibitory activities of leaf infusion. The aims of this research were to compare the antioxidant and α-glucosidase inhibitory activities of leaf infusion from guava, bay, and either as individual or combined infusions, and to analyze the chemical composition of the leaf infusion. &lt;strong&gt;Methods:&lt;/strong&gt; Air dried leaf powder of guava, bay and soursop were infused separately with boiled aquadest. The infusions were analyzed for their antioxidant activity against DPPH. The α-glucosidase inhibitory assay was conducted against α-glucosidase from &lt;em&gt;Saccharomyces cerevisiae. &lt;/em&gt;Then the infusions scanned with UV-Vis spectroscopy and analyzed with LC-MS. The synergism activities of the combined infusion were measured. &lt;strong&gt;Results:&lt;/strong&gt; Antioxidant activities of leaf infusions of guava and bay showed a comparable result IC&lt;sub&gt;50&lt;/sub&gt; 12.53 ± 0.55 and 10.76 ± 0.20 μg GAE/mL, but the infusion of soursop showed lower (IC&lt;sub&gt;50&lt;/sub&gt; 19.77 ± 0.35 μg GAE/mL) than BHT as positive control (11.6 ± 0.31 μg GAE/mL). If soursop infusion was not added, then the mixture of the guava and bay infusion showed an antioxidative synergistic effect. The α-glucosidase inhibitory activities of the guava, bay and soursop infusion (0.083 ± 0.01; 0.025 ± 0.007; 0.533 ± 0.039 μg GAE/mL, respectively) were stronger than acarbose (1285 ± 148 μg/mL). The α-glucosidase inhibitory activities of the combined infusions showed a synergistic effect. The main constituents of the guava infusion were identified tentatively as chrysin and caffeoylquinic acid, for the bay infusion it was caffeoylquinic, and for the soursop infusion it was luteolin. &lt;strong&gt;Conclusions:&lt;/strong&gt; There is a significant synergism of antioxidant activity of Guava and Bay mixture. The combined infusion of Bay and Soursop or Guava and Soursop showed antagonistic effect.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1269</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Ika Rahayu&lt;sup&gt;1&lt;/sup&gt;, Pamela Hendra Heng&lt;sup&gt;2&lt;/sup&gt;, Kris H. Timotius&lt;sup&gt;1&lt;/sup&gt;,*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biochemistry, Faculty of Medicine, Universitas Kristen Krida Wacana (UKRIDA), Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Psychology, Universitas Tarumanagara, Jakarta, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Syzygium polyanthum (Wight) Walp: A Potential Phytomedicine</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chemical</style></keyword><keyword><style  face="normal" font="default" size="100%">Nutritional</style></keyword><keyword><style  face="normal" font="default" size="100%">Pharmacological</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">Toxicological</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">429-438</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Ethnomedicinal plant is one of the important sources for drug discovery. &lt;em&gt;Syzygium polyanthum&lt;/em&gt; (Wight) Walp is one of the ethnomedicinal plants that is currently gaining attention for its various pharmacological potentials. This review evaluates its traditional uses, chemical profiles, toxicological aspects, nutritional values, as well as its pharmacological properties. From our literature search in Science Direct, Scopus, and Google Scholar databases, it was found that &lt;em&gt;S. polyanthum&lt;/em&gt; has valuable therapeutic potentials including antidiabetic, antihypertensive, antimicrobial, antioxidant, anticancer, antitumor, antidiarrheal, acetylcholinesterase, inhibitory, and dental plaque inhibition properties. However, few research areas, especially its toxicological profiles, mechanism of actions, as well as identification of its bioactive compound, require more in-depth studies.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Review Article</style></work-type><section><style face="normal" font="default" size="100%">429</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Azlini Ismail&lt;sup&gt;1*&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan, Pahang,&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Nurul Syahida Ramli</style></author><author><style face="normal" font="default" size="100%">Mahaneem Mohamed</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Acute and Sub-Acute Antihypertensive Effects of Syzygium polyanthum Leaf Extracts with Determination of Gallic Acid using HPLC Analysis</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antihypertensive</style></keyword><keyword><style  face="normal" font="default" size="100%">Gallic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Hypertension</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/647</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">663-671</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Context:&lt;/strong&gt; Aqueous decoction of &lt;em&gt;Syzygium polyanthum&lt;/em&gt; (ADSP) leaf is one of Malay traditionalremedial- preparations for hypertension. Intravenous ADSP reduced blood pressure of anesthetized Wistar-Kyoto (WKY) and Spontaneously-Hypertensive rats (SHR); however, acute and sub-acute effects of oral ADSP on conscious rats and its bioactive compound(s) are not comprehensively studied. This study aims to examine the acute and sub-acute effects of ADSP in comparison to methanol extract (MESP) on systolic blood pressure (SBP) of conscious WKY and SHR rats, as well as to identify their major phenolic-compound using highperformance- liquid-chromatography (HPLC). &lt;strong&gt;Methodology:&lt;/strong&gt; For acute study, SHR and WKY rats were gavaged with single-dose of ADSP or MESP (2.00, 2.50 or 3.00 g/kg), vehicles, or losartan (0.01 g/kg). SBP was measured after 1, 3, 5, 6 and 24 hr-post-administration. For subacute experiment, SHR rats were gavaged once-daily with ADSP or MESP (2.50 g/kg/day), vehicles, or losartan (0.01 g/kg/day) for 3-week and their SBP was weekly-measured. Phenolic compounds were screened using ferric-chloride test, then gallic acid was determined using HPLC. &lt;strong&gt;Results:&lt;/strong&gt; Acute administration of ADSP (2.50 to 3.00 g/kg) and MESP (2.00 to 3.00 g/ kg) significantly reduced SBP of SHR, but not of WKY rats. Repeated-daily-dose administration of MESP (2.5 g/kg/day) significantly reduced SBP of SHR after 2-week and sustained until 3-week, while ADSP (2.5 g/kg/day) significantly reduced SBP only after 3-week. Gallic acid was the major phenolic-compound in ADSP (157.09 &amp;plusmn; 4.18 ppm) and MESP (134.04 &amp;plusmn; 10.30 ppm). &lt;strong&gt;Conclusion:&lt;/strong&gt; Oral ADSP and MESP that contain gallic acid possess acute and sub-acute antihypertensive effects in SHR, but MESP&amp;rsquo;s effect was more prominent than ADSP&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">663</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Azlini Ismail&lt;sup&gt;1*&lt;/sup&gt;, Nurul Syahida Ramli&lt;sup&gt;2&lt;/sup&gt;, Mahaneem Mohamed&lt;sup&gt;3&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan Campus, Indera Mahkota, Kuantan, Pahang, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Biomedicine Program, School of Health Sciences, Health Campus, University Sains Malaysia, Kubang Kerian, Kelantan, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Physiology, School of Medical Sciences, Health Campus, University Sains Malaysia, Kubang Kerian, Kelantan, MALAYSIA.&lt;/p&gt;</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Erlena Nor Asmira Abd Rahim</style></author><author><style face="normal" font="default" size="100%">Azlini Ismail</style></author><author><style face="normal" font="default" size="100%">Muhammad Nor Omar</style></author><author><style face="normal" font="default" size="100%">Umi Nadhirah Rahmat</style></author><author><style face="normal" font="default" size="100%">Wan Amir Nizam Wan Ahmad</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">GC-MS Analysis of Phytochemical Compounds in Syzygium polyanthum Leaves Extracted using Ultrasound-Assisted Method</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bioactive compound</style></keyword><keyword><style  face="normal" font="default" size="100%">GC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Sonication</style></keyword><keyword><style  face="normal" font="default" size="100%">Syzygium polyanthum</style></keyword><keyword><style  face="normal" font="default" size="100%">Ultra-sound-assisted</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/406</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">110-119</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Context:&lt;/strong&gt; &lt;em&gt;Syzygium polyanthum&lt;/em&gt; has been traditionally formulated by the folklore for the treatment of diseases including diarrhea, rheumatism, diabetes mellitus, hypercholesterolemia, hypertension, gastritis and hyperuricemia. Normally, its phytochemicals are always extracted using solvent, maceration and steam distillation methods, but the use of ultrasound-assisted extraction (UAE) method is still not well documented. &lt;strong&gt;Aims:&lt;/strong&gt; This study aims to extract the phytochemical compounds present in &lt;em&gt;S. polyanthum&lt;/em&gt; leaves using UAE and to identify them by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. &lt;strong&gt;Methodology:&lt;/strong&gt; The leaves were consecutively soaked with n-hexane, ethyl acetate and methanol in a bath sonicator to derive n-hexane (HSP), ethyl acetate (EASP), and methanol (MSP) extracts of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves and then the extracts were subjected to GC-MS analysis. Mass-spectral databases of peaks were compared with database from Wiley, NIST and FNSCC libraries for compound identification. &lt;strong&gt;Results:&lt;/strong&gt; GC-MS analyses of HSP, EASP and MSP showed the presence of 21, 27, and 31 peaks, respectively. The major compound for HSP (31.912%), EASP (27.042%), and MSP (22.386%) were unknown compounds which were detected at retention time between 61.980 and 62.29 min, thus requires further characterization. Squalene and phytol were among the other major compounds present in all three extracts. Several identified compounds in the extracts such as squalene, phytol, hentriacontane, palmitic acid, &amp;alpha;-pinene, nerolidol, linalool, &amp;alpha;-tocopherol and &amp;beta;-tocopherol were known bioactive compounds. &lt;strong&gt;Conclusion:&lt;/strong&gt; GC-MS analyses of n-hexane (HSP), ethyl acetate (EASP), and methanol (MSP) of &lt;em&gt;S. polyanthum&lt;/em&gt; leaves extracts have revealed the presence of some known bioactive compounds with therapeutic importance.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">110</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Erlena Nor Asmira Abd Rahim&lt;sup&gt;1&lt;/sup&gt;, Azlini Ismail&lt;sup&gt;2&lt;/sup&gt;*, Muhammad Nor Omar&lt;sup&gt;1&lt;/sup&gt;, Umi Nadhirah Rahmat&lt;sup&gt;3&lt;/sup&gt;, Wan Amir Nizam Wan Ahmad&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biotechnology, Kuliyyah of Science, Kuantan Campus, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt; 2&lt;/sup&gt;Department of Fundamental Dental and Medical Sciences, Kuliyyah of Dentistry, Kuantan Campus, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, MALAYSIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;School of Health Sciences, Health Campus, UniversitiSains Malaysia, 16150 KubangKerian, Kelantan, MALAYSIA.&lt;/p&gt;</style></auth-address></record></records></xml>