<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Adrian Hutchings</style></author><author><style face="normal" font="default" size="100%">Ian Edwin Cock</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">An Interactive Antimicrobial Activity of Embelica officinalis Gaertn. Fruit Extracts and Conventional Antibiotics against Some Bacterial Triggers of Autoimmune Inflammatory Diseases</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Amla</style></keyword><keyword><style  face="normal" font="default" size="100%">ankylosing spondylitis</style></keyword><keyword><style  face="normal" font="default" size="100%">Combinational therapies</style></keyword><keyword><style  face="normal" font="default" size="100%">Indian gooseberry</style></keyword><keyword><style  face="normal" font="default" size="100%">Multi-drug resistant bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">multiple sclerosis</style></keyword><keyword><style  face="normal" font="default" size="100%">rheumatoid arthritis</style></keyword><keyword><style  face="normal" font="default" size="100%">Synergy</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/646</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">654-662</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;em&gt;Embelica officinalis&lt;/em&gt; Gaertn. is an Indian plant which is known for its therapeutic properties. It is especially well known as a component of the Ayuverdic medicine Triphala. This study focuses on the growth inhibitory activity of &lt;em&gt;E. officinalis&lt;/em&gt; fruit extracts against some bacterial triggers of autoimmune inflammatory diseases, both alone and in combination with conventional antibiotics. &lt;strong&gt;Methods:&lt;/strong&gt; &lt;em&gt;E. officinalis&lt;/em&gt; fruit powder was extracted with solvents of varying polarity and screened for bacterial growth inhibition by disc diffusion assay. The minimum inhibitory concentration (MIC) was quantified by both liquid dilution and disc diffusion techniques. To screen for combinatorial effects, the &lt;em&gt;E. officinalis&lt;/em&gt; fruit extracts were combined with a range of conventional antibiotics and tested against each bacteria using a liquid dilution assay. Toxicity was examined using &lt;em&gt;Artemia&lt;/em&gt; nauplii and HDF bioassays. &lt;strong&gt;Results:&lt;/strong&gt; The ethyl acetate &lt;em&gt;E. officinalis&lt;/em&gt; fruit extract displayed the strongest growth inhibitory activity against all of the bacterial triggers of autoimmune inflammatory disease. This extract was a particularly potent inhibitor of P. aeruginosa growth, with an MIC values as low as 264 &amp;mu;g/mL. The ethyl acetate extract was also a moderate to strong growth inhibitor of &lt;em&gt;P. mirabilis&lt;/em&gt;, &lt;em&gt;K. pneumonia&lt;/em&gt; and &lt;em&gt;A. baylyi&lt;/em&gt;, with MIC values generally 1000-1500 &amp;mu;g/mL. The methanolic and aqueous extracts also inhibited the growth of all bacteria, although generally with only moderate to low activity. Whilst no synergistic interactions were detected in combinations containing the &lt;em&gt;E. officinalis&lt;/em&gt; fruit extracts and conventional antibiotics, a number of combinations produced additive effects. These combinations are beneficial as they provide enhanced antibacterial efficacy compared to treatment with the antibiotic or extract components alone. No antagonistic interactions were detected. Therefore, use of the extracts in combination with conventional antibiotics would not compromise the antibiotics efficacy. All extracts were nontoxic in the &lt;em&gt;Artemia&lt;/em&gt; nauplii and HDF toxicity assays, further indicating their potential for medicinal use. &lt;strong&gt;Conclusion:&lt;/strong&gt; The &lt;em&gt;E. officinalis&lt;/em&gt; fruit extracts were moderate inhibitors of the bacterial triggers of selected autoimmune inflammatory diseases. Furthermore, the extracts potentiated the activity of chloramphenicol and tetracycline against otherwise resistant bacterial strains. Isolation of the active compounds and the potentiating agents may be beneficial in antibiotic drug design.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">654</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Adrian Hutchings&lt;sup&gt;1&lt;/sup&gt;, Ian Edwin Cock &lt;/strong&gt;&lt;sup&gt;&lt;strong&gt;1,2*&lt;/strong&gt; &lt;/sup&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;School of Natural Sciences, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, AUSTRALIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1,2&lt;/sup&gt;Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, AUSTRALIA.&lt;/p&gt;</style></auth-address></record></records></xml>