<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pathom Somwong</style></author><author><style face="normal" font="default" size="100%">Chomnapas Chuchote</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Determination of Lupeol, a Cytotoxic Compound Against SW620 Cells in the Extracts of Ha-Rak Recipe</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Benchalokawichian</style></keyword><keyword><style  face="normal" font="default" size="100%">Cancer Cell Lines</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Ha-Rak</style></keyword><keyword><style  face="normal" font="default" size="100%">Lupeol</style></keyword><keyword><style  face="normal" font="default" size="100%">MTT</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">133-138</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Ha-Rak is an herbal recipe that has been stated by Ministry of Public Health, Thailand to utilize as the herbal medicines for the treatment of fever. The recipe comprises the roots of five plants including&lt;em&gt; Capparis micracantha, Clerodendrum indicum, Ficus racemosa, Harrisonia perforata&lt;/em&gt; and&lt;em&gt; Tiliacora triandra&lt;/em&gt; as its ingredients. &lt;strong&gt;Methods:&lt;/strong&gt; The extracts of Ha-Rak and its five herbal principles were evaluated for their potential on the cytotoxicity against SW620 cancer cell lines using the MTT assay and an active compound, lupeol which was found as a cytotoxic compound in the herbal ingredients of Ha-Rak was further determined. A chromatographic method for investigating that compound in the extracts was developed herein. The analytical method comprised a cholesterol-coated C18 column, a mixture of acetonitrile and methanol (70:30) with a flow rate of 2 mL/min, and a diode-array UV wavelength at 210 nm as a stationary phase, mobile phase, and detector, respectively. &lt;strong&gt;Results: &lt;/strong&gt;The amount of lupeol in the extracts of Ha-Rak and the herbal principles, which possessed cytotoxicity against SW620 cell lines with the IC&lt;sub&gt;50 &lt;/sub&gt;values of 30.10-212.24 μg/mL displayed variously in the range of 4.50-250.62 mg/100g. The difference of the contents among the extracts was noted significantly (&lt;em&gt;P &lt;/em&gt;&amp;lt; 0.05). The correlation between the lupeol contents and their cytotoxic performances on the tested cell lines was observed in our investigation. &lt;strong&gt;Conclusion: &lt;/strong&gt;Our findings demonstrated that Ha-Rak recipe contains a promising cytotoxic compound, lupeol and it will support the recipe to subject further pharmacological studies on anticancer activity.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">133</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Pathom Somwong&lt;sup&gt;1,&lt;/sup&gt;*, Chomnapas Chuchote&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmaceutical Chemistry, College of Pharmacy, Rangsit University, Pathumthani, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacognosy, College of Pharmacy, Rangsit University, Pathumthani, THAILAND.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sangeetha D N</style></author><author><style face="normal" font="default" size="100%">S Rajamani</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In-vitro Cytotoxic Activity of Indianthus virgatus (Roxb.)Suksathan and Borchs. On A549, A431, CaCo2, U87 and L929 Cell Lines</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Cancer Cell Lines</style></keyword><keyword><style  face="normal" font="default" size="100%">Indianthus virgatus (Roxb.) Suksathan and Borchs</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicinal plant</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2018</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">1216-1220</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Medicinal plants play a key role to cure many diseases from time immemorial. The usage of medicinal plants in traditional medicinal system is the vital process of India. Cancer is one of the killing diseases and causes severe defects on human being. There are many types of cancer diseases in human beings affects the different organs. There is no proper medicine to cure such kind of cancer diseases. &lt;strong&gt;Objective:&lt;/strong&gt; The purpose of the study is to evaluate the test substances for their cytotoxicity against selected cell lines.&lt;strong&gt; Methods:&lt;/strong&gt; In the present study the&lt;em&gt; in-vitro&lt;/em&gt; cytotoxicity potential of chloroform and methanolic leaf extract of &lt;em&gt;Indianthus virgatus&lt;/em&gt; (Roxb.) Suksathan and Borchs. Was carried out against five cell lines, four of which were cancerous and one normal cell line i.e., A549, A431, CaCo2, U87 and L929. &lt;strong&gt;Results:&lt;/strong&gt; The results revealed that the cytotoxicity potential of the leaf and rhizome increased with the increase in concentration of leaf and rhizome extracts. The chloroform leaf extract showed highest percentage of growth inhibition against A549 cell line. The methanol leaf extract showed highest percentage of growth inhibition against A431 cell line. The chloroform leaf extract showed highest percentage of growth inhibition against CaCo2 cell line. The chloroform rhizome extract showed highest percentage of growth inhibition against U87 cell line. The methanolic leaf extract showed highest percentage of growth inhibition against L929 cell line .This shows that for different cell lines the highest percentage growth of inhibition was shown by different extracts. &lt;strong&gt;Conclusion:&lt;/strong&gt; The present study has suggested that the leaf and rhizome extracts of&lt;em&gt; Indianthus virgatus&lt;/em&gt; (Roxb.) Suksathan and Borchs. , Possesses potent anticancer property which can be used to prepare anticancer drug with proper standardization methods.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1216</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sangeetha D N&lt;sup&gt;1,&lt;/sup&gt; S. Rajamani&lt;sup&gt;2 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Botany, Research Scholar, Bharathair University, Coimbatore, Tamil Nadu INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Botany, Associate Professor St. Joseph&amp;rsquo;s Post Graduate and Research Centre, Langford road, Bengaluru, Karnataka, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>