<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Eko Susetyarini</style></author><author><style face="normal" font="default" size="100%">Poncojari Wahyono</style></author><author><style face="normal" font="default" size="100%">Sri Wahyuni</style></author><author><style face="normal" font="default" size="100%">Endrik Nurrohman</style></author><author><style face="normal" font="default" size="100%">Rahadian Zainul</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Impact of Stigmasterol from Beluntas Leaves (Pluchea indica) on SGOT and SGPT Levels in Male Rats (Rattus norvegicus)</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antifertility</style></keyword><keyword><style  face="normal" font="default" size="100%">Beluntas</style></keyword><keyword><style  face="normal" font="default" size="100%">Men</style></keyword><keyword><style  face="normal" font="default" size="100%">Safety Test</style></keyword><keyword><style  face="normal" font="default" size="100%">Stigmasterol</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">1311-1314</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Stigmasterol compounds have the potential as natural male antifertility drugs; based on the results of multiyear research and bioinformatics, further research is needed on the safety of stigmasterol preclinically. The study aimed to examine selected stigmasterol from blunt leaves that are safe for blood SGOT and SGPT levels the type of experimental research, the Post-test control design group. The study was conducted at the Biomedical Laboratory of the University of Muhammadiyah Malang, the Hufaka Bandung Laboratory, and the FKH Laboratory of Brawijaya University. The study was conducted from June to August 2024. The research sample of stigmasterol from blunt leaves came from the UPT materia medica of Batu City. The sampling technique was purposive random sampling. There were three dose treatments (0.125 mg/kgbb, 0.25 mg/kgbb, 0.5 mg/kgbb and control) with seven replications. Data analysis used one-way ANOVA and Duncan's further test. The results showed that giving various doses of stigmasterol could reduce SGPT in the blood but not SGOT. Duncan's test results of SGOT levels showed no significant difference between treatment categories; SGPT testing showed that the 0.125 mg/kgbb and 0.25 mg/kgbb treatments were not significantly different from the 0.5 mg/kg bb treatment but were substantially different from the control treatment. At the same time, the 0.5 mg/kgbb treatment was not significantly different from the other treatments. The conclusion and findings of the stigmasterol compound are in the safe category for use because it does not cause an increase in blood SGOT and SGPT and potentially tends to decrease.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1311</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Eko Susetyarini&lt;sup&gt;1*&lt;/sup&gt;, Poncojari Wahyono&lt;sup&gt;1&lt;/sup&gt;, Sri Wahyuni&lt;sup&gt;1&lt;/sup&gt;, Endrik Nurrohman&lt;sup&gt;1&lt;/sup&gt;, Rahadian Zainul&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Biology Education Study Program, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Malang, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dwisari Dillasamola</style></author><author><style face="normal" font="default" size="100%">Fatma Sri Wahyuni</style></author><author><style face="normal" font="default" size="100%">Rauza Sukma Rita</style></author><author><style face="normal" font="default" size="100%">Yufri Aldi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Immunostimulating Activity of Sungkai Leaf Stigmasterol Isolate against Cluster Differentiated 8+ T (CD8+T) from Covid 19 Disease Exposure</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cluster of Differentiation 8+ T (CD8+T)</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunostimulant.</style></keyword><keyword><style  face="normal" font="default" size="100%">SARS-CoV-2</style></keyword><keyword><style  face="normal" font="default" size="100%">Stigmasterol</style></keyword><keyword><style  face="normal" font="default" size="100%">Sungkai</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">738-742</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective: &lt;/strong&gt;Covid 19 is a disease that infects cells in the airway lining the alveoli. This disease is caused by a virus named SarsCoV2. One way to handle it is to increase immunity. The body itself consists of immune system organs. One component of the immune system that is very important in protecting the body against the SARS-Cov-2 virus attack is Cluster of Differentiation 8+ T (CD8+T). CD8+T is one of the components of the adaptive immune system or often known as the specific immune system and is cytotoxic, so it is called Cluster of Differentiation8 +T. One of the plants used by the community to increase endurance is Sungkai (&lt;em&gt;Peronema canescens &lt;/em&gt;Jack.). This study aims to see its activity against Cluster of Differentiation 8+ T (CD8+T).&lt;strong&gt; Methods:&lt;/strong&gt; Sungkai leaf powder was extracted using 70% ethanol solvent and evaporated using a rotary evaporator to obtain ethanol extract and fractionated with n-hexanes and isolated to obtain active isolates of sungkai leaves against the immune system. Isolates obtained from the isolation of the active fraction of n hexanes that have been tested as immunostimulants in previous studies. This study used test animals, namely male white mice of the wistar strain as many as 30 mice consisting of 5 heads for each group. Group I is a control group that is not given treatment and groups II, III, IV, V and VI are given exposure to the Sars CoV2 virus antigen that causes Covid 19 disease, namely using one of the Covid19 vaccines, namely the 0.0013 mL Moderna vaccine which is given Intra Muscular. Groups II, III, IV, V are treatment groups induced with Sars CoV2 antigen and given a test preparation, namely sungkai leaf isolate with 3 dose variations, namely 1, 10 and 100 mg/kg bw given orally, and group VI was given stimuno 50 mg/kgbw as a comparison. &lt;strong&gt;Results:&lt;/strong&gt; From the results of immunostimulant testing of sungkai leaf isolate, it was found that stigmasterol has an immunostimulant effect in increasing Cluster of Differentiation 8 + T (CD8 + T) in the body given SARS-CoV-2 virus antigen. Significant results were seen in the 100 mg/kg bw dose group which increased CD8+T by 53.93 ng/dl. Based on statistical tests conducted in the form of ANOVA tests, CD8+T also showed significant differences with a significance value of &amp;lt;0.05. &lt;strong&gt;Conclusion:&lt;/strong&gt; Stigmasterol isolate of sungkai leaf has the effect of increasing Cluster of Differentiation 8+ T (CD8+T) levels in the body given exposure to SARS-CoV 2 virus antigens.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">738</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Dwisari Dillasamola&lt;sup&gt;1&lt;/sup&gt;, Fatma Sri Wahyuni&lt;sup&gt;1&lt;/sup&gt;, Rauza Sukma Rita&lt;sup&gt;2&lt;/sup&gt;, Yufri Aldi&lt;sup&gt;1,*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Pharmacy, Universitas Andalas, Padang, Sumatera Barat, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Medicine, Universitas Andalas, Padang, Sumatera Barat, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Novia Delita</style></author><author><style face="normal" font="default" size="100%">Berna Elya</style></author><author><style face="normal" font="default" size="100%">Muhammad Hanafi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation and Identification of Chemical Compounds from Garcinia fruticosa Lauterb Stem Bark Extract</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">4-hydroxy-3-methoxy benzoic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Garcinia fruticosa</style></keyword><keyword><style  face="normal" font="default" size="100%">Isolation</style></keyword><keyword><style  face="normal" font="default" size="100%">Stigmasterol</style></keyword><keyword><style  face="normal" font="default" size="100%">Structural elucidation</style></keyword><keyword><style  face="normal" font="default" size="100%">β-sitosterol</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1641-1652</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Garcinia is a tropical plant that grows in Indonesia. Garcinia has many health benefits for the body. Garcinia contains many phenolic compounds and their derivatives, such as xanthon, flavonoids, benzophenone, lactone, and phenolic acids. &lt;em&gt;Garcinia fruticosa &lt;/em&gt;Lauterb. comes from the family Clusiaceae. The results of the phytochemical examination showed that &lt;em&gt;G. fruticosa &lt;/em&gt;bark extract contained alkaloids, flavonoids, glycosides, tannins, and saponins.&lt;strong&gt; Objective: &lt;/strong&gt;This study aims to isolate and identify chemical compounds from the ethyl acetate extract of &lt;em&gt;G. fruticosa &lt;/em&gt;Lauterb stem bark.&lt;strong&gt; Method: &lt;/strong&gt;&lt;em&gt;G. fruticosa &lt;/em&gt;Lauterb bark. dried, milled, and extracted with Step Gradient Polarity/SGP maceration using n-hexane, ethyl acetate, and methanol. Isolation was done by column chromatography and identified by thin layer chromatography and IR spectroscopy, LC-MS/MS, &lt;sup&gt;1&lt;/sup&gt;H-NMR, &lt;sup&gt;13&lt;/sup&gt;C-NMR, 2D-NMR (HSQC, HMBC). &lt;strong&gt;Results: &lt;/strong&gt;Compound D7a has a molecular weight 168.0496. The IR spectrum shows the presence of a group –OH appears on 3483 cm&lt;sup&gt;-1&lt;/sup&gt;, aromatic presence in 1609 cm&lt;sup&gt;-1&lt;/sup&gt;. The H-NMR spectrum shows the presence of aromatic signals on 6.96 (d, 8 Hz), 6.96 (d, 2 Hz) and 7.70 (dd, 8; 2 Hz). The C-NMR spectrum shows the presence of a carboxylic-COOH group appearing at 166.57 ppm, the presence of 2 x C-OH appearing at 147.18 and 151.18. In the HMBC spectrum, the -OCH&lt;sub&gt;3&lt;/sub&gt; position is located at C-3 with a correlation between the 3.79 (s) signal and the C signal at the chemical shift 147.18. &lt;strong&gt;Conclusions: &lt;/strong&gt;Structural elucidation shows that compound D7a is a 4-hydroxy-3-methoxy benzoate acid (Vanylic Acid) and isolate I-1 is an impure compound namely β-Sitosterol and Stigmasterol.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1641</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Novia Delita&lt;sup&gt;1,2&lt;/sup&gt;, Berna Elya&lt;sup&gt;3&lt;/sup&gt;*, Muhammad Hanafi&lt;sup&gt;4,5&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Graduate Programme of Biology Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacognosy- Phytochemistry, Faculty of Pharmacy and Science, Universitas Muhammadiyah Prof. Dr. Hamka, 1340 Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacognosy- Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Research Center for Chemistry, Indonesian Institute of Sciences, Serpong, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Faculty of Pharmacy, Universitas Pancasila, Jakarta, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nandhini S</style></author><author><style face="normal" font="default" size="100%">Ilango K</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Simultaneous Quantification of Lupeol, Stigmasterol and β- Sitosterol in Extracts of Adhatoda vasica Nees Leaves and its Marketed Formulations by a Validated RP-HPLC Method</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adhatoda vasica Nees</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Lupeol</style></keyword><keyword><style  face="normal" font="default" size="100%">Simultaneous quantification</style></keyword><keyword><style  face="normal" font="default" size="100%">Stigmasterol</style></keyword><keyword><style  face="normal" font="default" size="100%">β-sitosterol</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">850-856</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;Adhatoda vasica &lt;/em&gt;Nees (Acanthaceae) is a well-known medicinal plant used for the treatment of respiratory disorders such as asthma and bronchitis. &lt;strong&gt;Objective: &lt;/strong&gt;To develop a simple and precise RP-HPLC method for the simultaneous assessment of lupeol, stigmasterol and β-sitosterol of various extracts of &lt;em&gt;Adhatoda vasica &lt;/em&gt;Nees. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;The compounds were separated on RP-Phenomenex C&lt;sub&gt;18 &lt;/sub&gt;(250mm×4.6mm; 5μ) column with a mobile phase comprising of 0.1%v/v formic acid in water and methanol (28:82%v/v) splashed at a flow of 0.8mL/min with PDA detector at 208nm. &lt;strong&gt;Results: &lt;/strong&gt;The retention time of lupeol, stigmasterol and β-sitosterol was found to be 16.89, 18.26 and 20.72 minutes respectively. The amount of lupeol was abundant in hexane extract (0.952%w/w) and formulation III (23.72ng/g) whereas, stigmasterol (0.285%w/w) and β-sitosterol (8.649%w/w) was highly abundant in chloroform extract and formulation I stigmasterol (2.57ng/g) and β-sitosterol (0.98ng/g). The optimized method was validated for different parameters and all the validated constraints were within the limits as per ICH guidelines. The proposed method was linear over the concentration range of 12.5-200μg/mL with correlation coefficients greater than 0.997. The LOD and LOQ values of lupeol, stigmasterol and β-sitosterol were found to be 0.66, 5.64 and 12.8μg/mL and 2.01, 17.10 and 36.62μg/mL respectively. &lt;strong&gt;Conclusion: &lt;/strong&gt;To conclude, the developed method for the simultaneous estimation of lupeol, stigmasterol and β-sitosterol was simple, precise, accurate and thus reliable for the quality control investigations of crude drugs and its herbal formulations.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">850</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Nandhini S&lt;sup&gt;1&lt;/sup&gt;, Ilango K&lt;sup&gt;1,2,&lt;/sup&gt;* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Division of Pharmacognosy and Phytochemistry, Interdisciplinary Institute of Indian System of Medicine (IIISM), SRM Institute of Science and Technology, Kattankulathur - 603 203, Chengalpattu (Dt), Tamil Nadu, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur- 603 203, Chengalpattu (Dt), Tamil Nadu, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Choudhary D</style></author><author><style face="normal" font="default" size="100%">Shekhawat JK</style></author><author><style face="normal" font="default" size="100%">Kataria V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Callus</style></keyword><keyword><style  face="normal" font="default" size="100%">Campesterol</style></keyword><keyword><style  face="normal" font="default" size="100%">Capparidaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Fatty Acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Stigmasterol</style></keyword><keyword><style  face="normal" font="default" size="100%">Terpenoids</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">1055-1063</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;This study was designed to evaluate the phytocomponents present in aerial part and &lt;em&gt;in vitro &lt;/em&gt;induced callus methanol extracts of &lt;em&gt;Dipterygium glaucum&lt;/em&gt; by Gas Chromatography-Mass Spectrometry (GC-MS) technique. &lt;strong&gt;Methods: &lt;/strong&gt;GC-MS analysis of aerial part sample and callus produced from leaf of &lt;em&gt;D. glaucum &lt;/em&gt;extracted in methanol solvents was performed using GC-MS QP 2010 Plus (Shimadzu, Japan) system comprising an auto sampler (AOC-20i) and a gas chromatograph interfaced to a mass spectrometer. &lt;strong&gt;Results: &lt;/strong&gt;This study was carried out to identified and comparative analysis of bioactive phytochemicals from aerial part extract and callus extract of&lt;em&gt; D. glaucum&lt;/em&gt;. This analysis revealed that both the extracts have 69 different types of phytochemical components in varying quantities. Some of the important phytochemical compounds were Stigmasterol, β-sitosterol, γ-sitosterol, Campesterol, Squalene, n-Hexadecanoic acid, Stearic acid, Myristic acid, Quinazoline, Linalyl acetate etc. These chemical compounds have anticancer, antitumor, anti-inflammatory, antidiabetic and antioxidants properties. &lt;strong&gt;Conclusion:&lt;/strong&gt; This study represents the detection and identification of different phytochemical compounds from aerial part and callus extract of&lt;em&gt; D. glaucum&lt;/em&gt;. Thus, due to the presence of various important bioactive phytocomponents this plant is recommended as a pharmaceutically important plant.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1055</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Choudhary D, Shekhawat JK, Kataria V*&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;Biotechnology Unit, Department of Botany (UGC-Centre of Advanced Study), Jai Narain Vyas University, New Campus, Jodhpur 342001, Rajasthan, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vincent Antonio S. Ng</style></author><author><style face="normal" font="default" size="100%">Raymond S. Malabed</style></author><author><style face="normal" font="default" size="100%">Fernando B. Aurigue</style></author><author><style face="normal" font="default" size="100%">Consolacion Y. Ragasa</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Triterpenes and Sterols from Leaves of Hoya meliflua Merr</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apocynaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Hoya meliflua</style></keyword><keyword><style  face="normal" font="default" size="100%">Lupenone</style></keyword><keyword><style  face="normal" font="default" size="100%">Lupenyl Cinnamate</style></keyword><keyword><style  face="normal" font="default" size="100%">Lupeol</style></keyword><keyword><style  face="normal" font="default" size="100%">Oleanone</style></keyword><keyword><style  face="normal" font="default" size="100%">Squalene</style></keyword><keyword><style  face="normal" font="default" size="100%">Stigmasterol</style></keyword><keyword><style  face="normal" font="default" size="100%">Ursenone</style></keyword><keyword><style  face="normal" font="default" size="100%">α-amyrin</style></keyword><keyword><style  face="normal" font="default" size="100%">α-amyrin Cinnamate</style></keyword><keyword><style  face="normal" font="default" size="100%">β-amyrin</style></keyword><keyword><style  face="normal" font="default" size="100%">β-amyrin Cinnamate</style></keyword><keyword><style  face="normal" font="default" size="100%">β-sitosterol</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">48-52</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Hoya plants are also called wax plants due to the waxy appearance of their leaves and flowers. Most species are cultivated as ornamental plants. In the Philippines, there are at least 109 species of Hoya; 88 of these are endemic to the country. One of the endemic species is &lt;em&gt;Hoya meliflua&lt;/em&gt; often confused with &lt;em&gt;H. diversifolia&lt;/em&gt;, which can also be found in other countries. This study is part of our research on the chemical constituents of Philippine native Hoyas.&lt;strong&gt; Methods:&lt;/strong&gt; The compounds were isolated by silica gel chromatography and identified by NMR spectroscopy. &lt;strong&gt;Results:&lt;/strong&gt; Chemical investigation of the dichloromethane extract from the leaves of&lt;em&gt; Hoya meliflua&lt;/em&gt; afforded squalene and mixtures of β-amyrin (1a), α-amyrin (1b) and lupeol (1c) in about 1:1:0.25 ratio; oleanone (2a), ursenone (2b) and lupenone (2c) in about 1:1:0.3 ratio; β-amyrin cinnamate (3a), α-amyrin cinnamate (3b) and lupenyl cinnamate (3c) in about 0.5:0.3:1 ratio; and β-sitosterol and stigmasterol in about 5:1 ratio. &lt;strong&gt;Conclusion:&lt;/strong&gt; The results of our study indicate that &lt;em&gt;Hoya meliflua&lt;/em&gt; shares similar chemical characteristics with other members of the genus Hoya. The triterpenes and sterols obtained from H. meliflua were also identified from other &lt;em&gt;Hoya&lt;/em&gt; species. It is interesting to note that although most Hoya plants have no known biological activity and medicinal property, the compounds isolated from H. meliflua possess diverse bioactivities.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">48</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Vincent Antonio S. Ng&lt;sup&gt;1,*&lt;/sup&gt;, Raymond S. Malabed&lt;sup&gt;2&lt;/sup&gt;, Fernando B. Aurigue&lt;sup&gt;3&lt;/sup&gt;, Consolacion Y. Ragasa&lt;sup&gt;1,4 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Chemistry Department, De La Salle University, 2401 Taft Avenue, Manila 1004, PHILIPPINES.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Chemistry, Graduate School of Science, Osaka University, Osaka 560-0043, JAPAN.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;3&lt;/sup&gt;Agriculture Research Section, Atomic Research Division, Philippine Nuclear Research Institute-Department of Science and Technology, Commonwealth Avenue, Diliman, Quezon City 1101, PHILIPPINES.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;4&lt;/sup&gt;Chemistry Department, De La Salle University Science and Technology Complex Leandro V. Locsin Campus, Biñan City, Laguna 4024, PHILIPPINES.&lt;/p&gt;
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