<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Elsa Badriyya</style></author><author><style face="normal" font="default" size="100%">Salman</style></author><author><style face="normal" font="default" size="100%">Amalia Refina Pratiwi</style></author><author><style face="normal" font="default" size="100%">Dwisari Dillasamola</style></author><author><style face="normal" font="default" size="100%">Yufri Aldi</style></author><author><style face="normal" font="default" size="100%">Elidahanum Husni</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Topical Anti-Inflammatory Activity of Bromelain</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-inflammatory</style></keyword><keyword><style  face="normal" font="default" size="100%">Bromelain</style></keyword><keyword><style  face="normal" font="default" size="100%">Exudate</style></keyword><keyword><style  face="normal" font="default" size="100%">Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Granuloma</style></keyword><keyword><style  face="normal" font="default" size="100%">Leukocyte</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1586-1593</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Bromelain is a proteolytic enzyme found in pineapple plants. &lt;strong&gt;Aim:&lt;/strong&gt; This study conducted to determine the activity of bromelain enzyme as an anti-inflammatory using the Granuloma Pouch method. &lt;strong&gt;Methods: &lt;/strong&gt;The experimental animals were divided into five groups, namely the positive control group, the bromelain concentration group of 0.1%; 0.5%; 1%, and the comparison group. Experimental animals induced carrageenan 2% subcutaneously. The dosage form is given topically for 2, 4, and 6 days. The observation was made by measuring the volume of edema, the total number, and the percentage of leukocyte cells on days 2, 4, and 6. &lt;strong&gt;Results:&lt;/strong&gt; The observation results of a positive control group, bromelain concentration 0.1%, 0.5%, 1%, and the comparators, there was a significant decrease for the volume of exudate on days 2, 4, and 6 (p&amp;lt;0.05). The average total number of leukocytes also decreased significantly (p&amp;lt;0.05) in the positive control, the bromelain, and comparison group in day 2, 4, and 6. At the same time, the mean leucocyte percentage had no effect (p&amp;gt;0.05). &lt;strong&gt;Conclusion: &lt;/strong&gt;From the results of the study, it was concluded that the bromelain enzyme at concentration 0.1%, 0.5%, and 1% have an anti-inflammatory effect by suppressing the volume of exudate, and the total number of leucocyte.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1586</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Elsa Badriyya, Salman, Amalia Refina Pratiwi, Dwisari Dillasamola, Yufri Aldi, Elidahanum Husni* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Faculty of Pharmacy, University Andalas, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Novi Yantih</style></author><author><style face="normal" font="default" size="100%">Alfadella Methananda</style></author><author><style face="normal" font="default" size="100%">Yahdiana Harahap</style></author><author><style face="normal" font="default" size="100%">Wahono Sumaryono</style></author><author><style face="normal" font="default" size="100%">Lestari Rahayu</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Validation of High-Performance Liquid Chromatography for Determination of Bromelain in Pineapple (Ananas comosus (L) Merr) Water</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bromelain</style></keyword><keyword><style  face="normal" font="default" size="100%">HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Pineapple water</style></keyword><keyword><style  face="normal" font="default" size="100%">Storage</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">901-906</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective: &lt;/strong&gt;The aim of the present study was to validate HPLC method for analysis bromelain levels in pineapple water and application that method to determine the storage time of pineapple water. &lt;strong&gt;Methods:&lt;/strong&gt; The reversed phase of HPLC method was tested and optimized before it is validated. The composition and the flow rate of the mobile phase is the optimized parameter. The analytical parameters validated were detection limits, linearity, accuracy and precision. Pineapple water was stored for 8 h at 10ºC and bromelain was determined using the validated HPLC method.&lt;strong&gt; Result: &lt;/strong&gt;The optimum mobile phase composition was methanolwater (70:30) with a flow rate of 1 ml/min. The detector concentration-response was linear with coefficient of determination of 0.993. The accuracy of HPLC method at a recovery of 1 and 2% bromelain was 106.37 ± 1.94% and 98.12 ± 1.29% (n = 5), respectively. The precision, expressed as the coefficients of variation (CV), at 1 and 2% bromelain were 1.83 and 1.32% (n = 5), respectively. Bromelain level at zero time was 81.53%. After storage for 8 h at 10ºC, bromelain levels in pineapple juice appeared to decrease not statistically significant (p &amp;gt; 0.05), with to mean value of 78.46 ± 2.88%. &lt;strong&gt;Conclusion:&lt;/strong&gt; The HPLC method developed was valid to analyze accurately concentrations of bromelain in pineapple water and it can be used to study the shelf life of pineapple water based on bromelain content. The bromelain content in pineapple juice was not statistically significantly different after 8 h storage at 10°C.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">901</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Novi Yantih&lt;sup&gt;1,&lt;/sup&gt;*, Alfadella Methananda&lt;sup&gt;1&lt;/sup&gt;, Yahdiana Harahap&lt;sup&gt;2&lt;/sup&gt;, Wahono Sumaryono&lt;sup&gt;1&lt;/sup&gt;, Lestari Rahayu&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, INDONESIA.&lt;/p&gt;
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