<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sri Wardatun</style></author><author><style face="normal" font="default" size="100%">Yahdiana Harahap</style></author><author><style face="normal" font="default" size="100%">Abdul Mun'im</style></author><author><style face="normal" font="default" size="100%">Fadlina Chany Saputri</style></author><author><style face="normal" font="default" size="100%">Noorwati Sutandyo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Leucaena leucocephala (Lam.) de Wit Seeds: A New Potential Source of Sulfhydryl Compounds</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ethanol</style></keyword><keyword><style  face="normal" font="default" size="100%">Leucaena leucocephala (Lam.) de Wit</style></keyword><keyword><style  face="normal" font="default" size="100%">Mimosine</style></keyword><keyword><style  face="normal" font="default" size="100%">Sulfhydryl compounds</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">298-302</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;Leucaena leucocephala&lt;/em&gt; (Lam.) de Wit seeds are considered the most widely consumed legumes by ruminants. The seeds contain around 1.5 mmol of thiol/sulfhydryl compounds per 100 g of dried seeds. The contents of the sulfhydryl compounds can act like glutathione. On the other hand, the intake of a high amount of &lt;em&gt;Leucaena leucocephala &lt;/em&gt;(Lam.) de Wit seeds is limited by mimosine because it can induce toxicity and death in ruminants.&lt;strong&gt; Objective: &lt;/strong&gt;The aim of this study was to determine sulfhydryl compound levels in &lt;em&gt;Leucaena leucocephala&lt;/em&gt; (Lam.) de Wit seeds after the mimosine removal process. &lt;strong&gt;Materials and methods:&lt;/strong&gt; &lt;em&gt;Leucaena leucocephala&lt;/em&gt; (Lam.) de Wit seeds were soaked in aquadest for 24 hours and then dried at 40°C for 86 hours. The dried seeds were macerated at room temperature using 30%, 50%, 70%, and 96% (v/v) of ethanol as the solvent with a 1:10 solvent-to-solid ratio. Levels of dried extract yield were determined for sulfhydryl compounds and mimosine using a spectrophotometer.&lt;strong&gt; Results: &lt;/strong&gt;The 30% ethanol extract without soaking produced the highest levels of extract yield, but 70% ethanol was the most effective solvent for extracting the maximum sulfhydryl and minimum mimosine levels. &lt;strong&gt;Conclusion: &lt;/strong&gt;Ethanol solvent (70%) can be used to extract maximum levels of sulfhydryl compound and minimum levels of mimosine from &lt;em&gt;Leucaena leucocephala &lt;/em&gt;(Lam.) de Wit soaked seeds.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">298</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Sri Wardatun&lt;sup&gt;1&lt;/sup&gt;, Yahdiana Harahap&lt;sup&gt;1,&lt;/sup&gt;*, Abdul Mun'im&lt;sup&gt;2&lt;/sup&gt;, Fadlina Chany Saputri&lt;sup&gt;3&lt;/sup&gt;, Noorwati Sutandyo&lt;sup&gt;4&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Laboratory of Bioavailability and Bioequivalence, Faculty of Pharmacy, Universitas Indonesia, Depok 16242, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Laboratory of Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Laboratory of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Dharmais, Cancer Hospital, Jakarta, INDONESIA.&lt;/p&gt;
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