<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Peggy Willie</style></author><author><style face="normal" font="default" size="100%">Edak A Uyoh</style></author><author><style face="normal" font="default" size="100%">Peter O Aikpokpodion</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Gas Chromatography-Mass Spectrometry (GC-MS) Assay of Bio- Active Compounds and Phytochemical Analyses in Three Species of Apocynaceae</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drug discovery</style></keyword><keyword><style  face="normal" font="default" size="100%">Gongronema latifolium</style></keyword><keyword><style  face="normal" font="default" size="100%">Marsdenia edulis</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytochemical profiling</style></keyword><keyword><style  face="normal" font="default" size="100%">Underutilized species</style></keyword><keyword><style  face="normal" font="default" size="100%">Vincetoxicum rossicum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">383-392</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; Gas chromatography coupled with mass spectrometry (GC-MS) was used to analyze for phytochemicals and bioactive compounds in three species of Apocynacae, &lt;em&gt;Gongronema latifolium&lt;/em&gt;, &lt;em&gt;Vincetoxicum rossicum&lt;/em&gt; and &lt;em&gt;Marsdenia edulis&lt;/em&gt; commonly found in tropical rainforest vegetation and used as food and traditional medicine by locals. &lt;strong&gt;Methods and Materials: &lt;/strong&gt;Phytochemical analysis and GC-MS were carried out using leaf samples of the species following standard protocols. Quantitative phytochemical data were analyzed using analysis of variance (ANOVA) and significance tested at 5% level of probability. Bioactive compounds were identified by comparing the retention times with those of authentic compounds and spectral data obtained from National Institute of Standards and Technology (NIST) library.&lt;strong&gt; Results: &lt;/strong&gt;Phytochemical analysis revealed presence of alkaloids, glycosides, tannins, saponins, terpenes, steroids, flavonoids and phenols. Among the three species, &lt;em&gt;Gongronema latifolium&lt;/em&gt; was highest in flavonoids (28.40 %), &lt;em&gt;Vincetoxicum rossicum&lt;/em&gt; was highest in steroids (17.25 %) while &lt;em&gt;Marsdenia edulis&lt;/em&gt; was highest in terpenoids (18.17 %). GC-MS profiling of the species revealed biologically functional compounds with therapeutic properties including linoleic acid, phytol, neophytadiene, n-hexadecanoic acid, squalene, transfarnesol, 5-pentadecen-7-yne, and mercaptoacetic acid. &lt;strong&gt;Conclusion: &lt;/strong&gt;The array of bioactive compounds present in the three species especially &lt;em&gt;Gongronema latifolium&lt;/em&gt;, commonly used as food indicated their utility in pharmacognosy and drug manufacture. This is the first report of GC-MS based metabolite profiling to detect the various bioactive compounds in methanolic extracts of &lt;em&gt;Vincetoxicum rossicum&lt;/em&gt; and &lt;em&gt;Marsdenia edulis&lt;/em&gt;. We therefore recommend these species for further study in drug discovery trials.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">383</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Peggy Willie&lt;sup&gt;1&lt;/sup&gt;, Edak A. Uyoh&lt;sup&gt;1&lt;/sup&gt;, Peter O. Aikpokpodion&lt;sup&gt;1,&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Genetics and Biotechnology, Faculty of Biological Sciences, University of Calabar, PMB 1115 Cross River State, NIGERIA.&lt;/p&gt;
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