<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dayanand Dalawai</style></author><author><style face="normal" font="default" size="100%">Hosakatte Niranjana Murthy</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Chemical Profile and Antioxidant Properties of Andrographis producta (C. B. Clarke) Gamble</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">2</style></keyword><keyword><style  face="normal" font="default" size="100%">4-Di-tert-butylphenol</style></keyword><keyword><style  face="normal" font="default" size="100%">Andrographis</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidants</style></keyword><keyword><style  face="normal" font="default" size="100%">GC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Methanol extract</style></keyword><keyword><style  face="normal" font="default" size="100%">Salvigenin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">475-485</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;Andrographis producta&lt;/em&gt; (Acanthaceae) is endemic to Western Ghats, India, traditionally used by native people for the control of various ailments including intestinal worms, to relieve constipation and also used to eliminate phlegm in women during postpartum.&lt;strong&gt; Objective:&lt;/strong&gt; To investigate the chemical compounds in root, stem and leaves of&lt;em&gt; A. producta&lt;/em&gt; and their antioxidant properties. &lt;strong&gt;Method:&lt;/strong&gt; The phytochemical contents were determined using spectrophotometric methods and chemical profiling of root, stem and leaf extracts was carried out using GC-MS. Further, extracts were investigated for their antioxidant capacities using in vitro DPPH radical scavenging and FRAP assay.&lt;strong&gt; Results: &lt;/strong&gt;The total phenolics (163.61 mg GAE/g), flavonoids (35.11 ± 0.53 mg QE/g) and tannins (84.52 ± 0.07 mg TAE/g) were highest in stem compared to leaf and root. Stem was exerted superior antioxidant capacities in both DPPH (EC&lt;sub&gt;50 &lt;/sub&gt;3.58 mg/ml) and FRAP assays (1.742 ± 0.02 OD at 1mg/ml) and were comparable to standards. GC-MS analysis revealed total 89 chemical compounds including phenolics, flavonoids, terpenoids and organic acids. 2-Methoxy-4-vinylphenol (0.70 %), 2,4-ditert- butylphenol (9.74 %), phytol (10.32 %), 5-hydroxy-7,8-dimethoxyflavone (11.42 %), gammasitosterol (8.32 %), salvigenin (12.09 %), solanesol, (2.92 %), and alpha-terpinene (4.58 %) were important bioactive compounds found in significant amount. &lt;strong&gt;Conclusion: &lt;/strong&gt;The present investigations indicate that various parts of &lt;em&gt;A. producta&lt;/em&gt; can be explored as good source of antioxidants due to the presence of phenolics and flavonoids. The meticulous assessment of bioactive compounds from&lt;em&gt; A. producta&lt;/em&gt; would be great contribution in field of medicine.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">475</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Dayanand Dalawai, Hosakatte Niranjana Murthy*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Department of Botany, Karnatak University Dharwad 580003, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Medha A. Bhat</style></author><author><style face="normal" font="default" size="100%">Hosakatte Niranjana Murthy</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation, Characterization of Neoandrographolide from Andrographis macrobotrys Nees and Evaluation of its effect on LPS induced TNF-α Activity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Andrographis</style></keyword><keyword><style  face="normal" font="default" size="100%">Anti-inflammation</style></keyword><keyword><style  face="normal" font="default" size="100%">Terpenoids</style></keyword><keyword><style  face="normal" font="default" size="100%">THP-1 cells</style></keyword><keyword><style  face="normal" font="default" size="100%">TNF-α</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">669-674</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; &lt;em&gt;Andrographis macrobotrys&lt;/em&gt; Nees is an important species of genus &lt;em&gt;Andrographis&lt;/em&gt; with applications in traditional medicine. Neoandrographolide is one of the constituents in this plant. But there are no previous reports of isolation of neoandrographolide from &lt;em&gt;A. macrobotrys&lt;/em&gt;. Current work is undertaken to concentrate on isolation, characterization, and evaluation of tumor necrosis factor-alpha (TNF-α) inhibition activity of neoandrographolide from &lt;em&gt;A. macrobotrys&lt;/em&gt;. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;For the isolation process techniques like column chromatography, thin-layer chromatography (TLC), and preparative TLC were used. Characterization was done by ultra visible (UV)-visible spectroscopy, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H NMR), carbon-13 (C13) nuclear magnetic resonance (13C NMR) analysis. 3-(4,5-dimethylthiaxo-2yl) 2, 5-diphenyl tetrazolium bromide (MTT) assay was done for the preliminary cytotoxicity test to standardize the sample concentration for the TNF-α inhibition study. The flowcytometric method was used to determine TNF-α inhibiting ability in a human monocytes cell line (THP-1). &lt;strong&gt;Results:&amp;nbsp;&lt;/strong&gt;Neoandrographolide was isolated from methanolic extract of &lt;em&gt;A. macrobotrys&lt;/em&gt; which had a melting point of 174-175ºC. FTIR results had shown stretching for –OH, 3427.58 cm&lt;sup&gt;-1&lt;/sup&gt;,sp&lt;sup&gt;3&lt;/sup&gt;-CH, lactone, and α, β unsaturated ester. NMR data confirmed 26 carbon structures. Cytotoxicity of isolated neoandrographolide was 22.59 μg/ml. Further lipopolysaccharide (LPS) induced TNF-α inhibition was highest in the case of isolated neoandrographolide in comparison with the crude extract of&lt;em&gt; A. macrobotrys&lt;/em&gt;.&amp;nbsp;&lt;strong&gt;Conclusion:&lt;/strong&gt; &lt;em&gt;A. macrobotrys&lt;/em&gt; can be used as a new source of neoandrographolide with anti-inflammatory abilities by inhibiting the TNF-α release in THP-1 cells.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">669</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Medha A. Bhat, Hosakatte Niranjana Murthy* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Department of Botany, Karnatak University, Dharwad 580003, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Medha A. Bhat</style></author><author><style face="normal" font="default" size="100%">Hosakatte Niranjana Murthy</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation of Andrographolide from Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke and its Anticancer Activity against Human Ovarian Teratocarcinoma</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Andrographis</style></keyword><keyword><style  face="normal" font="default" size="100%">Andrographolide</style></keyword><keyword><style  face="normal" font="default" size="100%">Anticancer activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Ovarian teratocarcinoma</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">660-668</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Andrographolide is a well-known anticancer phytochemical often isolated from &lt;em&gt;Andrographis paniculata&lt;/em&gt; (Burm. f.) Nees. (Acanthaceae). Though &lt;em&gt;Andrographis lineata&lt;/em&gt; Wall. ex Nees var. lawii C.B. Clarke (ALw) which also belongs to the same family has an adequate amount of andrographolide; remained untouched for isolation of andrographolide and anticancer studies. Therefore, this study was targeted to isolate the andrographolide from the leaves of ALw and to assess its role inthe induction of apoptosis against the human ovarian teratocarcinoma (PA-1) cell line. &lt;strong&gt;Methods: &lt;/strong&gt;Column chromatography, thin-layer chromatography (TLC), preparative TLC were used for the isolation and purification while melting point, ultraviolet (UV)-visible spectroscopy, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H NMR), carbon-13 (C13) nuclear magnetic resonance (&lt;sup&gt;13&lt;/sup&gt;C NMR) analysis were carried out for characterization of the compound. 3-(4,5-dimethylthiaxo-2yl) 2, 5-diphenyl tetrazolium bromide (MTT) assay was carried out for cytotoxicity test and further Annexin-V staining, caspase 3 activity, B-cell lymphoma-2 (Bcl-2) activity, cell cycle analysis, and DNA damage study by terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) assays were carried out for apoptosis study. &lt;strong&gt;Results:&lt;/strong&gt; Andrographolide was isolated from the methanolic extract of leaves of ALw which had a melting point of 230 ºC, λ&lt;sub&gt;max&lt;/sub&gt; at 223 nm. FTIR results proved the presence of hydroxyl group, alkanes, carbon-carbon double bond, and a characteristic gamma lactone carbonyl. NMR data confirmed the 20 carbon structure. In the MTT assay cytotoxicity against PA-1 was at 3.7 μg/ml with other apoptotic assays supporting the induction of apoptosis by the compound at that concentration. &lt;strong&gt;Conclusion:&lt;/strong&gt; ALw is proved to be an alternate source of andrographolide with potential abilities to induce apoptosis in ovarian cancer cells.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">660</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Medha A. Bhat, Hosakatte Niranjana Murthy* &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Department of Botany, Karnatak University, Dharwad 580003, INDIA.&lt;/p&gt;
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