<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Eko Fuji Ariyanto</style></author><author><style face="normal" font="default" size="100%">Hermin Aminah Usman</style></author><author><style face="normal" font="default" size="100%">Ristaniah Rose Effendy</style></author><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic Effects of Imperata cylindrica Root Ethanol Extract on the Expression of PKCα, STAT3, and mTOR in A549 Lung Cancer Cell Line</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxic</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperata cylindrica</style></keyword><keyword><style  face="normal" font="default" size="100%">mTOR</style></keyword><keyword><style  face="normal" font="default" size="100%">PKCα</style></keyword><keyword><style  face="normal" font="default" size="100%">STAT3</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">284-291</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;em&gt;Imperata cylindrica&lt;/em&gt; or Cogon grass is a medicinal plant that has an anticancer effect. We unravel the cytotoxic effect of Imperata cylindrica root ethanol extract on the expression of PKCα, STAT3 and mTOR in A549 lung cancer cell lines. &lt;strong&gt;Methods: &lt;/strong&gt;The cytotoxic effect tests were carried out on the A549 lung cancer cell line after 48 hours post-treatment using 3- [4, 5-dimethylthiazol-2-yl] -2.5 diphenyl tetrazolium bromide (MTT) assay method to discover the IC&lt;sub&gt;50&lt;/sub&gt; dose, continued with apoptosis tests at doses of 150, 300, and 600 μg/mL of &lt;em&gt;I.cylindrica&lt;/em&gt; root ethanol extract using flowcytometry, as well as measurements of PKCα, STAT3, and mTOR mRNA expressions with the RT-qPCR method and measurements of PKCα, STAT3, and mTOR protein expressions with Western blot method. &lt;strong&gt;Results: &lt;/strong&gt;This study showed that the ethanol extract of &lt;em&gt;I. cylindrica&lt;/em&gt; roots had an IC&lt;sub&gt;50&lt;/sub&gt; of 541 μg/mL (weak cytotoxic) and the IC&lt;sub&gt;50 &lt;/sub&gt;of erlotinib was at 29 μM (moderate cytotoxic). In apoptotic test, the highest average apoptotic results were found in erlotinib group, while ethanol extract doses of 150, 300, and 600 μg/mL gave apoptotic effects on cancer cells higher than negative control group. From RT-qPCR, found that &lt;em&gt;I.cylindrica&lt;/em&gt; significantly inhibit the expression of PKCα, STAT&lt;sub&gt;3&lt;/sub&gt;, and mTOR mRNA and from band scanning using Western Blot showed PKCα, STAT3, and mTOR protein expressions in Imperata root ethanol extract, qualitatively produced thinner bands when compared to the negative control group. &lt;strong&gt;Conclusion:&lt;/strong&gt; This study demonstrated&lt;em&gt; I.cylindrica&lt;/em&gt; root ethanol extract ability inhibiting the expression of PKCα, STAT3 and mTOR genes and proteins in the A549 lung cancer cell line, which can be taken into consideration as a complementary therapy in treatment of lung cancer.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">284</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Raden Anita Indriyanti&lt;sup&gt;1,*&lt;/sup&gt;, Eko Fuji Ariyanto&lt;sup&gt;2&lt;/sup&gt;, Hermin Aminah Usman&lt;sup&gt;3&lt;/sup&gt;, Ristaniah Rose Effendy&lt;sup&gt;4&lt;/sup&gt;, Diah Dhianawaty&lt;sup&gt;2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Bandung Islamic University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department Pathology Anatomy, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&amp;nbsp;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Radiology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Eko Fuji Ariyanto</style></author><author><style face="normal" font="default" size="100%">Hermin Aminah Usman</style></author><author><style face="normal" font="default" size="100%">Ristaniah Rose Effendy</style></author><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Synergistic Effect of Imperata cylindrica Root Extract and Erlotinib on A549 Lung Cancer Cell Viability</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">A549</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell viability</style></keyword><keyword><style  face="normal" font="default" size="100%">Erlotinib</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperata cylindrica</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">656-659</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Erlotinib is typically used to treat lung adenocarcinoma and Imperata cylindrica extract has been shown to exert anti-cancer effects in various human cancer cell lines. Therefore, this study evaluated the combined anticancer effects of Imperata cylindrica ethanol root extract and erlotinib on the A549 lung cancer cell line. &lt;strong&gt;Method:&lt;/strong&gt; The A-549 lung cancer cell line was treated with various combinations of 1/2 IC50, 3/8 IC50, 1/4 IC50, and 1/8 IC50 of I.cylindrica root ethanol extract and erlotinib for 48 hours. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, analyzed and interpreted using CompuSyn software and a normalized isobologram curve. &lt;strong&gt;Result:&lt;/strong&gt; Of the sixteen combinations, eleven combinations acted synergistically, four were additive, and one was in the antagonist zone. &lt;strong&gt;Conclusion:&lt;/strong&gt; The combination of I. cylindrica root ethanol extract and erlotinib act synergistically to decrease A549 lung cancer cell viability, therefore they are potential lung cancer therapeutics.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">656</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Raden Anita Indriyanti&lt;sup&gt;1&lt;/sup&gt;*, Eko Fuji Ariyanto&lt;sup&gt;2&lt;/sup&gt;, Hermin Aminah Usman&lt;sup&gt;3&lt;/sup&gt;, Ristaniah Rose Effendy&lt;sup&gt;4&lt;/sup&gt;, Diah Dhianawaty&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Bandung Islamic University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;3&lt;/sup&gt;Department Pathology Anatomy, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Radiology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, INDONESIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Eko Fuji Ariyanto</style></author><author><style face="normal" font="default" size="100%">Hermin Aminah Usman</style></author><author><style face="normal" font="default" size="100%">Ristaniah Rose Effendy</style></author><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Quantification of total polyphenols and flavonoids, antioxidant activity, and Sinensetin and Imperatorin contents of Imperata cylindrica root ethanol extract</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Flavonoid</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperata cylindrica</style></keyword><keyword><style  face="normal" font="default" size="100%">Polyphenol</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">327-337</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt;&lt;em&gt;&lt;strong&gt; &lt;/strong&gt;Imperata cylindrica&lt;/em&gt;, commonly known as cogon grass, is currently widely distributed and used as a medicinal plant. The major compounds that have been isolated and identified are polyphenols and flavonoids, which have many biological activities such as antioxidant, and anticancer. Polyphenols and flavonoids are mostly found in the roots and leaves. This study aimed to perform phytochemical screening on &lt;em&gt;I. cylindrica&lt;/em&gt; root ethanol extract from Sragen, Central Java, Indonesia and determine the total polyphenol, flavonoid, antioxidant activity and quantify Sinensetin and Imperatorin contents of the extract.&lt;strong&gt; Method:&lt;/strong&gt; Quantification of all parameters were measured using visible spectrophotometric methods. Total polyphenol, total flavonoid contents, as well as antioxidant activity were measured using Folin-Ciocalteu reagent, aluminum chloride reagent, and 1,1-diphenyl-2-picrylhydrazyl, respectively, and quantification of Sinensetin and Imperatorin were measured using High Performance Liquid Chromatography. &lt;strong&gt;Results:&lt;/strong&gt;&lt;em&gt; I cylindrica&lt;/em&gt; root ethanol extract had a total polyphenol content of 1.109% gallic acid equivalent, total flavonoid content of 0.1% quercetin equivalent, and antioxidant activity IC&lt;sub&gt;50&lt;/sub&gt; 824.30 μg/ml. Sinensetin and Imperatorin were also identified in Fractions 1 to 11 with concentrations of 0.0157 and 0.0178 mg/kg extract, respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; &lt;em&gt;I. cylindrica &lt;/em&gt;root ethanol extract from Sragen had active phytochemical compounds of polyphenols, flavonoids, and antioxidants as well as Sinensetin and Imperatorin.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article </style></work-type><accession-num><style face="normal" font="default" size="100%">11</style></accession-num><section><style face="normal" font="default" size="100%">327</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Raden Anita Indriyanti&lt;sup&gt;1,2*&lt;/sup&gt;, Eko Fuji Ariyanto&lt;sup&gt;3&lt;/sup&gt;, Hermin Aminah Usman&lt;sup&gt;4&lt;/sup&gt;, Ristaniah Rose Effendy&lt;sup&gt;5&lt;/sup&gt;, Diah Dhianawaty&lt;sup&gt;3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacology, Faculty of Medicine, Bandung Islamic University, Bandung, West Java, Indonesia&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Doctoral Program in Medical Science, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department Pathology Anatomy, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Radiology, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Diah Dhianawaty</style></author><author><style face="normal" font="default" size="100%">Mas Rizky Anggun Adipurna Syamsunarno</style></author><author><style face="normal" font="default" size="100%">Resti Gradia Dwiwina</style></author><author><style face="normal" font="default" size="100%">Raden Anita Indriyanti</style></author><author><style face="normal" font="default" size="100%">Ruslin</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Separation and Quantification of Sinensetin, Imperatorin and Total Tannin Content as Active Phytoconstituents of Methanol Extract of Imperata cylindrica Root from Kendari</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Imperata cylindrica</style></keyword><keyword><style  face="normal" font="default" size="100%">Imperatorin</style></keyword><keyword><style  face="normal" font="default" size="100%">Sinensetin</style></keyword><keyword><style  face="normal" font="default" size="100%">Tannin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">September 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">1216-1224</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt;&lt;em&gt; Imperata cylindrica&lt;/em&gt; root methanol extract harvested from Kendari had anti-hypertensive activity stated in a study on hypertension rat models at the doses 60 and 90 mg/kg-bw. The extract also contained phenolic compound 1.53% (GAE) and flavonoid 1.17% (QE) and had antioxidant activity IC&lt;sub&gt;50 &lt;/sub&gt;0.32 mg/mL. Aim of the study was to determine the active secondary metabolites in the extract. &lt;strong&gt;Materials and Method: &lt;/strong&gt;Sinensetin and imperatorin in the extract were separated on silica gel 60 column, eluted with a gradient eluent system as follows (1) n-hexane- ethyl acetate (3:7); (2) n-hexane – ethyl acetate (2:8); (3) ethyl acetate -MeOH (8:2), and (4) methanol. Imperatorin and sinensetin in fractions were identified and quantified by HPLC method with column LiChroCART 250-4,6 RP 18E, isocratic mobile phases with the composition of mixture as follows, 0.1% formic acid, acetonitrile and methanol (40:50:10) respectively, and at temperature 400 C. The total tannin was determined by spectrophotometry method with Fe(III) chloride and 1.10-Phenanthroline at wave length 510 nm. &lt;strong&gt;Results:&lt;/strong&gt; Imperatorin and sinensetin were identified in fractions 5 to 9. The concentrations of sinensetin and imperatorin in &lt;em&gt;Imperata cylindrica&lt;/em&gt; root methanol extract were of 32.348 and 37.014 mg/kg extract, respectively. The total tannin content was 1.06 % (w/w) (TAE). &lt;strong&gt;Conclusion:&lt;/strong&gt; &lt;em&gt;Imperata cylindrica &lt;/em&gt;root methanol extract from Kendari had active phytoconstituents, namely sinensetin, imperatorin, and tannin.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1216</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Diah Dhianawaty&lt;sup&gt;1,&lt;/sup&gt;*, Mas Rizky Anggun Adipurna Syamsunarno&lt;sup&gt;1&lt;/sup&gt;, Resti Gradia Dwiwina&lt;sup&gt;2&lt;/sup&gt;, Raden Anita Indriyanti&lt;sup&gt; 3&lt;/sup&gt;, Ruslin&lt;sup&gt;4&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biomedical Sciences, Division of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biomedical Sciences, Division of Cell Biology, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacology, Bandung Islamic University, Bandung, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Faculty of Pharmacy, Halu Oleo University, Kendari, Southeast Sulawesi, INDONESIA.&lt;/p&gt;
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