<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Horacio Larqué</style></author><author><style face="normal" font="default" size="100%">Abelardo Chávez Montes</style></author><author><style face="normal" font="default" size="100%">Jaime Zamora-Chimal</style></author><author><style face="normal" font="default" size="100%">Moises Looh-Hernández</style></author><author><style face="normal" font="default" size="100%">Joel H. Elizondo Luevano</style></author><author><style face="normal" font="default" size="100%">Esther del Olmo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bioguided Assay of Polyphenols Isolated from Medicinal Mayan Species and its Activity Against Leishmania mexicana.</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Coumarins</style></keyword><keyword><style  face="normal" font="default" size="100%">Flavonoids</style></keyword><keyword><style  face="normal" font="default" size="100%">Leishmania mexicana</style></keyword><keyword><style  face="normal" font="default" size="100%">NMR structural determination</style></keyword><keyword><style  face="normal" font="default" size="100%">Polyphenols</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">174-180</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Objective: &lt;/strong&gt;This study underlines the in vitro leishmanicidal activity of the methanol extracts (MeOH), fractions of n-hexane (n-Hex), chloroform (TCM) and ethyl acetate (EtOAc), and compounds isolated from plant species used in the Mayan traditional medicine. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;Extracts of medicinal species collected in the Mayan Peninsula such as Hylocerus undatus, Bauhinia divaricate, Euphorbia hirta, Ruellia nudiflora and Cedrela odorata, were tasted in a bio guided assays against amastigotes of Leishmania mexicana. Different chromatographic techniques were applied in order to isolated the most active compounds. Additionally, spectroscopic experiments 1H-NMR, 13C-NMR, LC-MS and FT-IR were stablished to determine the chemical structure of the chemical compounds. &lt;strong&gt;Results: &lt;/strong&gt;Euphorbia hirta and Cedrella odorata, showed good bioactivity with 14.81 ± 2.63 g/mL and IC50 = 18.39 ± 0.88 μg/mL respectively, meanwhile Bauhinia divaricata not show activity and Ruellia nudiflora showed poor activity with IC50 = 92.18 ± 3.64 μg/mL, followed by Hylocerus undatus with IC50 = 122.5 ± 20.99 μg/mL, when tasted against amastigotes of Leishmania mexicana. Spectroscopic data confirmed the presence of quercetin, myricetin, kempherol and scopoletin, with IC50 = 2.92 ± 0.42 μM, 12.30 ± 0.57 μM, 20.22 ± 4.66 μM and 4.05 ± 0.68 μM respectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; The bioguided assays guided us, to the purification and isolation of four different metabolites, mainly flavonoids and structurally related compounds, some of them show good activity, however, their low bioavailability indicates the need for detailed structural relation activity studies, together with the development of formulations and delivery systems.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">174</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Horacio Larqué&lt;sup&gt;1,2&lt;/sup&gt;,*, Abelardo Chávez Montes&lt;sup&gt;2&lt;/sup&gt;, Jaime Zamora-Chimal&lt;sup&gt;3&lt;/sup&gt;, Moises Looh- Hernández&lt;sup&gt;3&lt;/sup&gt;, Joel H. Elizondo Luevano&lt;sup&gt;2&lt;/sup&gt;, Esther del Olmo&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Departamento de Química Farmacéutica, Facultad de Farmacia, Universidad de Salamanca (USAL), Campus Miguel de Unamuno s/n, 37007, Salamanca, SPAIN.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Av. de los Rectores s/n, Cd. Universitaria, 66450, San Nicolás de los Garza, Nuevo León, MÉXICO.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, MÉXICO.&lt;/p&gt;
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