<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sreelakshmi Bada Venkatappa Gari</style></author><author><style face="normal" font="default" size="100%">Ramalingam Peraman</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Tinospora Sinensis (Lour.) Merr. Stem Modulate The TNF-Alpha Expression In HCT- 116 Tumour Cell, Besides the Inhibitory Effect on Cervical, Colon and Breast Cancer Cell Lines and Mycobacterium Tuberculosis H37Rv</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Antitubercular</style></keyword><keyword><style  face="normal" font="default" size="100%">HCT-116</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunomodulatory</style></keyword><keyword><style  face="normal" font="default" size="100%">Tinospora sinensis</style></keyword><keyword><style  face="normal" font="default" size="100%">TNF-Alpha</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">8-16</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;The present study was designed to evaluate TNF-Alpha experession, anticancer and antitubercular properties for the stem extracts of &lt;em&gt;Tinospora sinensis&lt;/em&gt; (TS). &lt;strong&gt;Objective: &lt;/strong&gt;natural product research is widely used for identifying hit molecules for life threatening diseases including cancer, tuberculosis and drug resistant infections. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; There were three polarity dependant solvent extracts obtained through cold maceration process using ethanol (ELTS), ethyl acetate (EATS) and n-hexane (NHTS), respectively. The extracts were subjected to MTT assay for their anticancer potential against HeLa (cervical cancer), MCF-7 (breast cancer) and HCT116 (colon cancer) cell lines, and based on the results, NHTS was subjected to flow cytometry for TNF-Alpha expression in HCT-116 cells. The antitubercular activity for the extracts was performed against &lt;em&gt;Mycobacterium tuberculosis&lt;/em&gt; H&lt;sub&gt;37&lt;/sub&gt;Rv (Mtb) by luciferase reporter phage (LPS) assay method.&lt;strong&gt; Results:&lt;/strong&gt; The result of anticancer screening revealed that n-hexane extracts showed the significant inhibition (&lt;em&gt;p&lt;/em&gt;&amp;lt;0.05) on HCT-116 cells with the IC&lt;sub&gt;50&lt;/sub&gt; of 177.4 μg/ml, whereas EATS and ELTS were equally active on HeLa with the respective IC&lt;sub&gt;50&lt;/sub&gt; of 236 and 277 μg/ml. The NHTS was significantly effective on decreasing (&lt;em&gt;P&lt;/em&gt;&amp;lt;0.05) TNF-Alpha expression (31.27 MFU) in HCT-116 cells and is closely active with standard simvastatin (26.7 MFU) against the control (7.06 MFU). The antitubercular activity results revealed the equi-potency of both NHTS and EATS on Mtb with growth inhibition of 84 % at 100μg/ml. The GC-MS analyses of NHTS confirmed the presence of Berberine, palmatine, tembertarine, magniflorine, choline and tinosporin. &lt;strong&gt;Conclusion: &lt;/strong&gt;Overall, we scientifically support the traditional use&lt;em&gt; Tinospora sinensis&lt;/em&gt; stem in the treatment of cancer and immune diseases.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">8</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Sreelakshmi Bada Venkatappa Gari&lt;sup&gt;1,&lt;/sup&gt;*, Ramalingam Peraman&lt;sup&gt;2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Research Scholar, Faculty of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Anantapur (JNTUA), Anantapur, Andhra Pradesh 515002, INDIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Professor of Pharmaceutical and Medicinal chemistry RERDS-Centre for Pharmaceutical Research, Raghavendra Institute of Pharmaceutical Education and Research (RIPER)-Autonomous, Anantapur, Andhra Pradesh 515721, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mariquit M. De Los Reyes</style></author><author><style face="normal" font="default" size="100%">Glenn G. Oyong</style></author><author><style face="normal" font="default" size="100%">Vincent Antonio S. Ng</style></author><author><style face="normal" font="default" size="100%">Chien-Chang Shen</style></author><author><style face="normal" font="default" size="100%">Consolacion Y. Ragasa</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic Compounds from Kibatalia gitingensis (Elm.) Woodson</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apocynaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">HCT-116</style></keyword><keyword><style  face="normal" font="default" size="100%">HDFn</style></keyword><keyword><style  face="normal" font="default" size="100%">HT-29</style></keyword><keyword><style  face="normal" font="default" size="100%">Isoscopoletin</style></keyword><keyword><style  face="normal" font="default" size="100%">Kibatalia gitingensis</style></keyword><keyword><style  face="normal" font="default" size="100%">Lupeol acetate</style></keyword><keyword><style  face="normal" font="default" size="100%">MCF-7</style></keyword><keyword><style  face="normal" font="default" size="100%">PrestoBlue® cell viability assay.</style></keyword><keyword><style  face="normal" font="default" size="100%">Squalene</style></keyword><keyword><style  face="normal" font="default" size="100%">Ursolic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">α-amyrin acetate</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2016</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">8-13</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;Ursolic acid (&lt;strong&gt;1&lt;/strong&gt;), squalene (&lt;strong&gt;2&lt;/strong&gt;), a mixture of &amp;alpha;-amyrin acetate (&lt;strong&gt;3a&lt;/strong&gt;) and lupeol acetate (&lt;strong&gt;3b&lt;/strong&gt;), and isoscopoletin (&lt;strong&gt;4&lt;/strong&gt;), isolated from the dichloromethane extracts of the leaves and twigs of &lt;em&gt;Kibatalia gitingensis&lt;/em&gt;, were evaluated for their cytotoxic activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast-neonatal (HDFn), using the&lt;em&gt; in vitro&lt;/em&gt; PrestoBlue&lt;sup&gt;&amp;reg;&lt;/sup&gt; cell viability assay. Compounds &lt;strong&gt;1-4&lt;/strong&gt; exhibited strong cytotoxic activities against HT-29 cells with IC&lt;sub&gt;50&lt;/sub&gt; values ranging from 0.6931 to 1.083 &amp;mu;g/mL. Furthermore, &lt;strong&gt;1-4 &lt;/strong&gt;were moderately cytotoxic against HCT-116 cells with IC&lt;sub&gt;50&lt;/sub&gt; values ranging from 4.065 to 11.09 &lt;em&gt;&amp;mu;g&lt;/em&gt;/mL. These compounds were least cytotoxic against MCF-7 cells with IC&lt;sub&gt;50&lt;/sub&gt; values ranging from 8.642 to 25.87 &lt;em&gt;&amp;mu;g&lt;/em&gt;/mL. The most cytotoxic against HT-29 cells, HCT-116 cells and MCF-7 cells are &lt;strong&gt;2, 4&lt;/strong&gt; and &lt;strong&gt;1&lt;/strong&gt;, respectively.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">8</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Mariquit M. De Los Reyes&lt;sup&gt;1,2&lt;/sup&gt;, Glenn G. Oyong&lt;sup&gt;3&lt;/sup&gt;, Vincent Antonio S. Ng&lt;sup&gt;4&lt;/sup&gt;, Chien-Chang Shen&lt;sup&gt;5&lt;/sup&gt;, Consolacion Y. Ragasa&lt;sup&gt;4,6&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Biology Department, De La Salle University Science &amp;amp; Technology Complex, Leandro V. Locsin Campus, Bi&amp;ntilde;an City, Laguna 4024, PHILIPPINES.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Biology Department, De La Salle University, 2401 Taft Avenue, Manila 0922, PHILIPPINES.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Center for Natural Science and Environmental Research, De La Salle University, 2401 Taft Avenue, Manila 0922, PHILIPPINES.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Chemistry Department, De La Salle University, 2401 Taft Avenue, Manila 0922, PHILIPPINES.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1, Li-Nong St., Sec. 2, Taipei 112, TAIWAN.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Chemistry Department, De La Salle University Science &amp;amp; Technology Complex, Leandro V. Locsin Campus, Bi&amp;ntilde;an City, Laguna 4024, PHILIPPINES.&lt;/p&gt;
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