<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tanvi Pingale</style></author><author><style face="normal" font="default" size="100%">Pallavi Duse</style></author><author><style face="normal" font="default" size="100%">Sunita Ogale</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antibacterial and Antifungal Approaches of Ficus racemosa</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anthraquinone glycosides.</style></keyword><keyword><style  face="normal" font="default" size="100%">Ficus racemosa</style></keyword><keyword><style  face="normal" font="default" size="100%">Moraceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Soyabean casein digest agar</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">355-357</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;em&gt;Ficus racemosa&lt;/em&gt; also called as Ficus glomerata Roxb. is a species of plant in the family Moraceae. Popularly known as the Audumbar, cluster fig tree, Indian fig tree or goolar (gular). Different parts of plant shows Antibacterial, Antitussive, Anthelmintic, Antidiarrhoeal, Anticancer, Anti-inflammatory activities etc. on various extracts. Latest and previous studies have concluded the beneficial aspects of fruit of the plant shows Antimicrobial, Antibacterial and Antifungal activity using different cultures and extracts. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; The method was adopted for preparation of plant extracts. The media used for antibacterial test was Nutrient agar/broth. The culture medium was inoculated with the microorganism separately suspended in nutrient broth. The antibacterial activity was evaluated by measuring the diameter of zone of inhibition observed. The broth dilution method was adopted for determination of MIC value against the pathogens. &lt;strong&gt;Results and Discussion:&lt;/strong&gt; The ethanoic and ethyl acetate extract showed more promising antimicrobial activity as compared to Water, Hexane and Chloroform extract. In well diffusion method, the ethyl acetate extract had showed significant bactericidal activity.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">355</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Tanvi Pingale&lt;sup&gt;1*&lt;/sup&gt;, Pallavi Duse&lt;sup&gt;2&lt;/sup&gt;, Sunita Ogale&lt;sup&gt;3 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Assistant Professor in Pharmacology, VIVA Institute of Pharmacy, Virar, Maharashtra, INDIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Assistant Professor in Quality Assurance, VIVA Institute of Pharmacy, Virar, Maharashtra, INDIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;3&lt;/sup&gt;VIVA Institute of Pharmacy, Virar, Maharashtra, INDIA.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sowmyashree Gangaraju</style></author><author><style face="normal" font="default" size="100%">Bhagyalakshmi Manjappa</style></author><author><style face="normal" font="default" size="100%">Girish Kesturu Subbaiah</style></author><author><style face="normal" font="default" size="100%">Kemparaju Kempaiah</style></author><author><style face="normal" font="default" size="100%">Rangaiah Shashidharamurthy</style></author><author><style face="normal" font="default" size="100%">Jane Hoover Plow</style></author><author><style face="normal" font="default" size="100%">Sebastin Santhosh Martin</style></author><author><style face="normal" font="default" size="100%">Manohar Shinde</style></author><author><style face="normal" font="default" size="100%">Devaraja Sannaningaiah</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Jackfruit (Artocarpus heterophyllus) seed extract exhibits fibrino(geno)lytic activity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Fibrino(geno)lysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Hemostasis</style></keyword><keyword><style  face="normal" font="default" size="100%">Jackfruit</style></keyword><keyword><style  face="normal" font="default" size="100%">Moraceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Non-hemorrhagic</style></keyword><keyword><style  face="normal" font="default" size="100%">Serine/cysteine protease.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">01/2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">171-177</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;&lt;strong&gt;Objective: &lt;/strong&gt;The current study assesses the fibrinogen and fibrin clot hydrolyzing activities of aqueous seed extract of Jackfruit (AqSEJ). &lt;strong&gt;Methods:&lt;/strong&gt; The protein banding pattern of AqSEJ (100 &amp;mu;g) was analyzed on SDS-PAGE. The proteolytic activity of AqSEJ was confirmed by spectrophotometer and zymography experiments. Fibrinogen, fibrin and plasma protein hydrolyzing activities of AqSEJ were analyzed on SDS-PAGE under reduced conditions. Plasminogen activation and indirect hemolytic activities was analyzed using spectrophotometer. The non-toxic property of AqSEJ was tested by edema, hemorrhage in experimental mice. &lt;strong&gt;Results:&lt;/strong&gt; AqSEJ exhibited proteolytic activity and the specific activity was found to be 1.04 units/mg/min. Furthermore, AqSEJ non-specifically hydrolyzed A&amp;alpha;, followed by B&amp;beta; and &amp;gamma; chains of human fibrinogen and specifically hydrolyzed &amp;alpha; polymer and &amp;alpha; chain of partially cross linked human fibrin clot without affecting &amp;beta; chain and &amp;gamma;-&amp;gamma; dimer even up to the tested dose of 30 &amp;micro;g for the incubation period of 8 hours. Importantly, AqSEJ did not hydrolyze other plasma proteins and devoid of plasminogen activation property. The proteolytic activity of AqSEJ was completely neutralized by PMSF and IAA, while EDTA, EGTA, 1,10-Phenanthroline did not, suggesting the presence of serine and cysteine family proteases. Moreover, AqSEJ did not cause edema and hemorrhage in experimental mice up to the tested dose of 200 &amp;micro;g and non-toxic to RBC cells. &lt;strong&gt;Conclusion:&lt;/strong&gt; AqSEJ hydrolyzes fibrinogen and fibrin clot and non-toxic in nature. Hence, this work showcases the potential applications of Jack fruit seed proteases in the treatment of thrombotic disorders.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">171</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sowmyashree Gangaraju&lt;sup&gt;1&lt;/sup&gt;, Bhagyalakshmi Manjappa&lt;sup&gt;1&lt;/sup&gt;, Girish Kesturu Subbaiah&lt;sup&gt;1&lt;/sup&gt;, Kemparaju Kempaiah&lt;sup&gt;2&lt;/sup&gt;, Rangaiah Shashidharamurthy&lt;sup&gt;3&lt;/sup&gt;, Jane Hoover Plow&lt;sup&gt;4&lt;/sup&gt;, Sebastin Santhosh Martin&lt;sup&gt;5&lt;/sup&gt;, Manohar Shinde&lt;sup&gt;1&lt;/sup&gt;, and Devaraja Sannaningaiah&lt;sup&gt;1*&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1,*&lt;/sup&gt;Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur- 572103, India.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Studies in Biochemistry, University of Mysore, Manasagangothry, Mysore-570 006, India.&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmaceutical Sciences, Philadelphia College of Osteopathic Medicine. Atlanta, Georgia, USA .&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, Cleveland Clinic Lerner Research Institute, Cleveland, OH 5Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 17177, Stockhlm, Sweden.&lt;/p&gt;
</style></auth-address></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sowmyashree Gangaraju</style></author><author><style face="normal" font="default" size="100%">Bhagyalakshmi Manjappa</style></author><author><style face="normal" font="default" size="100%">Girish Kesturu Subbaiah</style></author><author><style face="normal" font="default" size="100%">Kemparaju Kempaiah</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Jackfruit (Artocarpus heterophyllus) seed extract exhibits fibrino(geno)lytic activity</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Fibrino(geno)lysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Hemostasis</style></keyword><keyword><style  face="normal" font="default" size="100%">Jackfruit</style></keyword><keyword><style  face="normal" font="default" size="100%">Moraceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Non-hemorrhagic</style></keyword><keyword><style  face="normal" font="default" size="100%">Serine/cysteine protease.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">09th Mar, 2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">171-177</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; The current study assesses the fibrinogen and fibrin clot hydrolyzing activities of aqueous seed extract of Jackfruit (AqSEJ).&lt;strong&gt; Methods:&lt;/strong&gt; The protein banding pattern of AqSEJ (100 &amp;mu;g) was analyzed on SDS-PAGE. The proteolytic activity of AqSEJ was confirmed by spectrophotometer and zymography experiments. Fibrinogen, fibrin and plasma protein hydrolyzing activities of AqSEJ were analyzed on SDS-PAGE under reduced conditions. Plasminogen activation and indirect hemolytic activities was analyzed using spectrophotometer. The non-toxic property of AqSEJ was tested by edema, hemorrhage in experimental mice. &lt;strong&gt;Results: &lt;/strong&gt;AqSEJ exhibited proteolytic activity and the specific activity was found to be 1.04 units/mg/min. Furthermore, AqSEJ non-specifically hydrolyzed A&amp;alpha;, followed by B&amp;beta; and &amp;gamma; chains of human fibrinogen and specifically hydrolyzed &amp;alpha; polymer and &amp;alpha; chain of partially cross linked human fibrin clot without affecting &amp;beta; chain and &amp;gamma;-&amp;gamma; dimer even up to the tested dose of 30 &amp;micro;g for the incubation period of 8 hours. Importantly, AqSEJ did not hydrolyze other plasma proteins and devoid of plasminogen activation property. The proteolytic activity of AqSEJ was completely neutralized by PMSF and IAA, while EDTA, EGTA, 1,10-Phenanthroline did not, suggesting the presence of serine and cysteine family proteases. Moreover, AqSEJ did not cause edema and hemorrhage in experimental mice up to the tested dose of 200 &amp;micro;g and nontoxic to RBC cells. &lt;strong&gt;Conclusion: &lt;/strong&gt;AqSEJ hydrolyzes fibrinogen and fibrin clot and non-toxic in nature. Hence, this work showcases the potential applications of Jack fruit seed proteases in the treatment of thrombotic disorders.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; Fibrino(geno)lysis, Hemostasis, Jackfruit, Moraceae, Non-hemorrhagic, Serine/cysteine protease.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">171</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sowmyashree Gangaraju&lt;sup&gt;1&lt;/sup&gt;, Bhagyalakshmi Manjappa&lt;sup&gt;1&lt;/sup&gt;, Girish Kesturu Subbaiah&lt;sup&gt;1&lt;/sup&gt;, Kemparaju Kempaiah&lt;sup&gt;2&lt;/sup&gt;, Rangaiah Shashidhara Murthy&lt;sup&gt;3&lt;/sup&gt;, Jane Hoover Plow&lt;sup&gt;4&lt;/sup&gt;, Sebastin Santhosh Martin&lt;sup&gt;5&lt;/sup&gt;, Manohar Shinde&lt;sup&gt;1&lt;/sup&gt;, and Devaraja Sannaningaiah&lt;sup&gt;1*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1,*&lt;/sup&gt;Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur- 572103, India&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Departments of Studies in Biochemistry, University of Mysore, Manasagangothry, Mysore-570 006, India&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmaceutical Sciences, Philadelphia College of Osteopathic Medicine. Atlanta, Georgia, USA&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, Cleveland Clinic Lerner Research Institute, Cleveland, OH&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 17177, Stockhlm, Sweden&lt;/p&gt;</style></auth-address></record></records></xml>