In vitro Evaluation of Anthelmintic Activity of Gymnema sylvestre Plant

The evaluation of anthelmintic activity of Gymnema sylvestre was performed by bioassay method where hot and cold hydroalcoholic extracts were used against earthworm (Pheretima posthuma). The anthelmintic assay was carried out as the 50ml formulations containing five different concentrations of each cold and hot hydroalcholic extracts (25, 50, 100 ,250 and 500mg/ml in distilled water) were prepared and six worms (same type) were placed in them. Time for paralysis and death time was noted. Albendazole (20mg/ml) was used as a reference standard, while normal saline as the control.