Bioactive Constituents of Pulicaria jaubertii: A promising Antihypertensive Activity

Objective: Phytochemical investigation and evaluation of antihypertensive activity of the alcoholic extract of Pulicaria jaubertii aerial parts. Material and Methods: The chloroform and ethyl acetate fractions were chromatographed on silica gel and sephadex LH 20 to afford six compounds. Their structures were elucidated on the bases of extensive NMR (¹H, ¹³C, DEPT, COSY, HSQC, HMBC, NOESY) and MS analysis. The antihypertensive activity of the alcoholic extract was evaluated against L-NAME–induced hypertensive rats. Results: One new monoterpene glucoside; (2R, 4S)-p-menth-1-ene- 2-O-b-D-glucopyranoside [1] along with five known compounds; thymoquinol 2-O-b-D-glucopyranoside (zataroside-B) [2], quercetin 7, 3`-di-Omethylether (rhamnazin) [3], quercetin 7-O-methylether (rhamnetin) [4], quercetin 3`-O-methylether (isorhamnetin) [5] and stigmasterol 3-Ob- D-glucopyranoside [6] were isolated. The alcoholic extract significantly reduced the elevated SBP and partially restored the diminished nitric oxide metabolites. Conclusion: Compound [1] is a new and compounds [2-6] are reported for first time from Pulicaria jaubertii. The possible antihypertensive activity of the alcoholic extract may be due to its flavonoids and phenolic contents.