ArticleViewAbstractPharmacognosy Journal,2014,6,6,10-14.Published:3rd Sept, 2014Type:Original ArticleHigh-Performance Thin-Layer Chromatography Fingerprinting of Ethnopharmacological Important Seeds of Wrightia tinctoriaRajani Srivastava, Alok Mukerjee, and Amita Verma Rajani Srivastava1*, Alok Mukerjee2, Amita Verma11Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences-Deemed University, Allahabad, Uttar Pradesh, India,2United Institute of Pharmacy, Allahabad, Uttar Pradesh, IndiaAbstract:Introduction:Wrightia tinctoria R. Br. belongs to family Apocynaceae commonly called as sweet Indrajao, Pala indigo plant, Dyer’s Oleander. “Jaundice curative tree” in south India. Materials and Methods: A method has been developed for different extracts of W. tinctoria for high-performance thin-layer chromatography (HPTLC) fingerprinting analysis for identification and quantification of the marker compound. Result: The satisfactory resolution was obtained in the solvent system toluene:ethyl acetate v/v (8:2) for petroleum ether extract, toluene:ethyl acetate v/v (7:3) for chloroform extract and toluene:ethyl acetate:formic acid v/v (7:3:0.1) for alcoholic extract. Conclusion: The HPTLC fingerprinting profile developed for different extracts of W. tinctoria will help in proper identification and quantification of the marker compound.Key words: High-performance thin-layer chromatography fingerprinting, Wrightia tinctoria, petether extract, chloroform extract, alcoholic extract.Keywords:Alcoholic extract, chloroform extract, High-performance thin-layer chromatography fingerprinting, petether extract, Wrightia tinctoria