ArticleViewAbstractPharmacognosy Journal,2019,11,2,388-394.DOI:10.5530/pj.2019.11.60Published:February 2019Type:Original ArticleComparative DNA Fingerprinting and Botanical Study of Certain Haworthia and Gasteria Species Growing in EgyptAli Mohamed El Shamy, Seham Salah El Din El Hawary, Heba Ahmed Fahmy, and Shahira Mohammed Ezzat Ali Mohamed El Shamy1, Seham Salah El Din El Hawary1, Heba Ahmed Fahmy2,*, Shahira Mohammed Ezzat1,3 1Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr El-Einy Street, 11562, Cairo, EGYPT. 2Department of Pharmacognosy, Faculty of Pharmacy, Modern University for Technology and Information, Cairo, EGYPT. 3Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 6th October, 12566, EGYPT. Abstract:Introduction: Haworthia Duval and Gasteria Duval are succulent plants, native to South Africa. They have been used worldwide in folk medicine. They are members of the Alooideae subfamily, which has been always viewed as a taxonomically confusing sub-family. The present study aims to differentiate between Haworthia limifolia Marloth, Gasteria carinata (Mill.) Duval and Gasteria minima Poelln. growing in Egypt. Methods: A comparative botanical and genetic investigation is presented. Macromorphological and micromorphological botanical features of the leaves and stems of the species under investigation using the entire specimens, transverse sections and isolated elements. Moreover, their DNA was extracted from the leaf samples and RAPD-PCR analysis was made using 10 primers. Results: The comparative botanical criteria of the examined leaves and stems were identified. Furthermore, the total number of amplified products produced by the ten primers was 98 fragments. Primers OPB-07, OPB-01 and OPB-02, recording high percentage of polymorphism (78.6, 70.6 and 57.1%, respectively), can be used to differentiate between the examined species. While primers OPB-01, OPB-04, OPB-07 and OPB-08, generating fragments with wide molecular size (17, 14, 14 and 11fragments, respectively), can be used for the identification of Haworthia limifolia, Gasteria carinata and Gasteria minima. Conclusion: The macromorphological, micromorphological and genetic identifying parameters presented in the current study revealed good crieteria to authenticate and differentiate between Haworthia limifolia Marloth, Gasteria carinata (Mill.) Duval and Gasteria minima Poelln. Keywords:Gasteria, Haworthia, Macromorphology, Micromorphology, RAPD-PCRView:PDF (1.19 MB) PDF Images Morphology of H. limifolia, A: aerial part (x =0.2), B: leaves (x=0.25) ‹ Preparation and Evaluation of Nutraceutical Product Mixture of Seeds of Cucumis melo, Punica granatum, Linum usitatissimum, for Antioxidant, Prebiotic and Nutraceutical Potential up Phytochemical Constituents and Free Radical Scavenging Activity of Madang Gatal (Schima wallichii) Choisy Stem Bark ›