Flavonoids in Passiflora incarnata L. Dry Extract of Russian Origin

Background: Flavonoids are one of the main classes of biologically active substances providing the pharmacotherapeutic effect of passionflower (Passiflora incarnata L.) preparations. In this article studies on the standardization of Passiflora incarnata L. dry extract (PDE) by flavonoids are presented. The aim of this work was to study the composition and content of flavonoids in PDE with the help of precise modern physicochemical methods. Materials and Methods: PDE was prepared from crude herbal drug – P. incarnata herb of Russian origin. Reverse phase HPLC-UV analysis was performed with Agilent 1100 liquid chromatograph. Chromatographic column was Atlantis C₁₈ (250 mm × 4.6 mm × 5 μm); analytical wavelength – 350 nm; mobile phase – 0.01% formic acid solution and methanol: acetonitrile (25:75); column temperature – 35°C; analysis time – 90 min; flow rate of the mobile phase – 0.8 ml/min in gradient elution mode. Commercially available samples of flavonoids were used for identification and quantitative determination. Results: 20 compounds of flavonoid structure are presented in the PDE. 9 flavonoid compounds have been identified, they are: isovitexin, vitexin, rutoside, hyperoside, luteolin, kaempferol, kaempferitrin, orientin, and isoorientin. The content of vitexin is 0,867 ± 0,011%, the total flavonoids content in terms of vitexin is 3,762 ± 0,049%. Conclusion: The obtained data will be used to create regulatory documentation for drugs based on PDE.