ArticleViewAbstractPharmacognosy Journal,2021,13,5,1225-1241.DOI:10.5530/pj.2021.13.156Published:September 2021Type:Research ArticlePhytochemical Screening and Anti-Inflammatory Potential of the Organic Extracts from Cleoserrata serrata (Jacq.) Iltis.María del Carmen Juárez-Vázquez, Alejandro Zamilpa A, Rosalba León-Díaz, Mariano Martínez-Vázquez, Adolfo López-Torres, Julieta Luna-Herrera, Lilian Yépez-Mulia, Francisco Alarcón-Aguilar, and María Adelina Jiménez-Arellanes María del Carmen Juárez-Vázquez1,2†, Alejandro Zamilpa A3, Rosalba León- Díaz4, Mariano Martínez-Vázquez5, Adolfo López-Torres6, Julieta Luna- Herrera7, Lilian Yépez-Mulia8, Francisco Alarcón-Aguilar2, María Adelina Jiménez-Arellanes1,* 1Unidad de Investigación Médica (UIM) en Farmacología, UMAE Hospital de Especialidades, Centro Médico Nacional Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS). Av. Cuauhtémoc 330, Col. Doctores 06720, Ciudad de México (CdMx), MEXICO. 2Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, CdMx 09340, MEXICO. 3Centro de Investigación Biomédica del Sur (CIBIS), IMSS, Argentina 1, Col. Centro 062790, Xochitepec, Morelos, MEXICO. 4Área de Biología y Salud Integral, Instituto de Investigaciones Biológicas, Universidad Veracruzana. Luis Castelazo s/n. Col. Industrial Ánimas, Xalapa 91190, Veracruz, MEXICO. 5Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, Coyoacán 04510, CdMx, MEXICO. 6Instituto de Biotecnología, Universidad del Papaloapan. Circuito Central 12200, Col. Parque Industrial, Tuxtepec 68301, Oaxaca, MEXICO. 7Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, CdMx 11340, MEXICO. 8UIM en Enfermedades Infecciosas y Parasitarias, UMAE Hospital de Pediatria, CMN-SXXI, IMSS. Av. Cuauhtémoc 330, Col. Doctores 06720, CdMx, MEXICO. Abstract:Cleoserrata serrata is used in Mexican-south traditional medicine to treat chicleros ulcer. The phytochemical profile and the anti-inflammatory effect from four extracts obtained by maceration proccess and of the primary fractions from two extracts are described. In addition, the antioxidant, leishmanicidal and antimycobaterial activities and LD50 from active extracts are reported. Anti-inflammatory activity was evaluated in TPA and carrageenan assays. Ethyl palmitate and γ-sitosterol were detected in Hexanic and CH2Cl2 extracts. Same compounds and stigmasta-3,5-dien-7-one, palmitic acid, phytol acetate and phytol were detected in primary fractions from CH2Cl2:EtOH extract. In this and in MeOH extracts a polyphenolmixture was obtained. The MeOH extract was subjected to acid hydrolysis, and kaempferol, quercetin and scopoletin were detected in organic-phase. Polyphenol-mixture and organic-phase (IC50=3730 and 2338 μg/mL) showed moderate antioxidant activity; meanwhile MeOH extract exhibited scarce activity. In carrageenan model, Hexanic extract and polyphenol-mixture showed ED50=131.46 and 64.89 mg/ kg, respectively. Three extracts were active but not-dose-dependent. In TPA-model, CH2Cl2 extract and polyphenol-mixture showed ED50 <0.79 mg/ear, and three extracts were active, however the effect was not-dose-dependent. CH2Cl2:EtOH showed antimycobacterial and leishmanicidal activities. The LD50 was >2 g/kg for all extracts. Keywords:Anti-inflammatory Effect, Antimycobacterial activity, antioxidant activity, Cleoserrata serrata, DL50, Leishmanicidal activity, Organic extractView:PDF (1.13 MB) PDF Images Graphical Abstract ‹ Separation and Quantification of Sinensetin, Imperatorin and Total Tannin Content as Active Phytoconstituents of Methanol Extract of Imperata cylindrica Root from Kendari up Microwave-Assisted Extraction and Identification of γ- Oryzanol from Rice Bran (Oryza sativa L. cv Ciliwung) ›