Immunomodulator Activity of Ethanol Extract of Tapak Liman Leaves (Elephantopus scaber Linn.)

Introduction: Tapak Liman (Elephantopus scaber Linn) is a medicinal plant in Indonesia which traditionally used as a treatment for fever, gout, dysentery, hepatitis, and infections. Aim: This study aims to determine the immunostimulants activity of tapak liman extract with carbon clearance method and to determine the percentage and total leukocytes and relative lymph weights. Materials and Methods: Twenty white male mice divide assigned to 1 control group and 3 experimental groups. It treated by oral administration of tapak liman extract; 10, 30, and 100 mg/kgBW. After six days of administration, mice intravenously injected with 0.1 ml/10gBW colloidal carbon. Blood obtained from the mice tail at; 3rd, 6th, 9th, 12th and 15th minutes. The determined absorbance then calculated to obtain the phagocytotic index, the percentage and the total of leukocyte cells and lymph weights. Results: Phagocytosis index increased significantly at 1, 12, 1.24 and 1.47. The percentages of neutrophil segment are 57%, 60% and 60%, for lymphocytes are 3, 80%, 32.80% and 34.20%, monocyte cells are 4.60%, 3.00% and 2.60%, neutrophil are 3.40%, 3.20%,2.20%, and for the eosinophil cells are 3.20%, 1.8% and 1.20%. Total leukocyte cells are 10,760, 11,630 and 15,880 cells/microliter. The relative lymph weight of each dose in the sequence is 0.36, 0.49 and 0.66. Conclusion: Ethanol extract of tapak liman leaves (Elephantopus scaber Linn) can increase the immunity of mice.