Hypoxic Preconditioning Decrease ROS and Increase SOD Expression in Adipose-Derived Mesenchymal Cell

Adipose-derived Mesenchymal Stem Cells (AMSCs) have promising ability to differentiate into a cardiomyocyte. However, post-transplantation survival of AMSCs is relatively low due to lethal cellular hypoxia. Hypoxic preconditioning is a sublethal hypoxia condition which may improve AMSCs survival. This research evaluates the effect of hypoxic preconditioning on the expression of reactive oxygen species (ROS) and superoxide dismutase (SOD) of AMSCs. Isolated human AMSCs was cultured to the 4^th passage and confirmed with CD45, CD90 and CD105 expression. Cells were divided into control group (normoxia with 21% O₂) and hypoxic preconditioning group (with 1% O₂). ROS and SOD were evaluated using immunofluorescence and analyzed using SPSS 25. AMSCs was characterized by the CD105 and CD90 without expression of CD44 and CD45. ROS expression is significantly lower in hypoxia group than in controlled group (253,13 ± 67,795 vs 342,13 ± 116,447; p < 0.05) and SOD expression is significantly higher in hypoxia group than in controlled group (340,25 ± 96,476 vs 234,56 ± 38,238; p <0.05). In conclusion, hypoxic preconditioning in human AMSCs induce lower expression of intracellular ROS and higher expression of intracellular SOD.