Drug of Action Cassia Alata Leaves Extract as Antiviral to Dengue Virus Serotype-2 in vitro

Background and Objectives: Dengue viruses (DENV) is a mosquito-borne members of the Flaviridae family.To date, more than 2.5 billion people in over 100 countries are at risk of infection, and approximately 20 million infections were reported annually. Currently, There is no specific antiviral treatment available for DENV infection. Natural products possess a wide range of biological and biochemical potential. Among them, plants are one of the most important sources for discovering new drugs for therapy. Our previous study, showed that Cassia alata has potency as antiviral to DENV, however drug of action still unclear. Materials and Methods: We explore the drug of action of C. alata leaves exract and its fraction through time of addition studies and effect of solvents wtih the dose based on the previous study. Results: The most effective inhibition druf of action was determined by focus assay. Meanwhile the toxicity was measured by MTT assay. These studies demosntrated that ethanol extract of Cassia alata 1 mg/ml showed strong inhibition in both early step (receptor and attachment to host cells) and post infection with inhibition 96.04 % and 99.16 %.Compared with those fractions, Cassia alata ethanol extract has strongest inhibition DENV in every step of virus replication. Conclussion: Cassia alata ethanol extract has strongest inhibition DENV in every step of virus replication with the average of inhibition more than 95 %. Ethyl acetate and hexane has strongest inihibition with the average of inhibition 100 %.