ArticleViewAbstractPharmacognosy Journal,2020,12,4,857-863.DOI:10.5530/pj.2020.12.123Published:June 2020Type:Research ArticleDetermination of the Chemical Composition of Lady’s Bedstraw (Galium verum L.) Herb Extract by GC-MSZaichikova SG, Bokov DO, Kiselevskii MV, Antsyshkina AM, Bondar AA, Prostodusheva TV, Shchepochkina OYu, and Gegechkori VI Zaichikova SG1, Bokov DO1,2,*, Kiselevskii MV3, Antsyshkina AM1, Bondar AA1, Prostodusheva TV1, Shchepochkina OYu1, Gegechkori VI1 1A.P. Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University, 8 Trubetskaya St., bldg. 2, Moscow, 119991, RUSSIAN FEDERATION. 2Laboratory of Food Chemistry, Federal Research Center of Nutrition, Biotechnology and Food Safety, 2/14 Ustyinsky pr., Moscow, 109240, RUSSIAN FEDERATION. 3Laboratory of Cell-Mediated Immunity, Blokhin National Medical Research Center of Oncology, 24, Kashirskoye sh., Moscow, 115478, RUSSIAN FEDERATION. Abstract:Background: The research is devoted to the study of the Lady’s bedstraw (Galium verum L.) herb ethanol extract composition using the method of gas chromatography with mass spectrometric detection. Materials and Methods: Air-dried G. verum L. herb was used for ethanol extract preparation. Hewlett Packard 6890, 5973A GC/MSD System equipped with an HP-5MS quartz capillary column with geometric dimensions of 30 m×0.25 mm×0.25 μm was used for GC-MS analysis. Results: It was established that the Lady’s bedstraw ethanol extract is rich in biologically active substances that determine its wide spectrum of pharmacological action. 15 compounds were identified by comparing with library mass spectra. Conclusions: G. verum L. is a promising source of crude herbal drugs. In the future, after additional pharmacognostic studies, G. verum L. herb can be recommended for implementation in the State Pharmacopeia of the Russian Federation. Keywords:Ethanol Extract, Galium verum L., Gas chromatography-mass spectrometry, Lady’s bedstrawView:PDF (3.94 MB) PDF Images Lady’s bedstraw (Galium verum L.): flowering plant (1), crude herbal drug (2) ‹ Simultaneous Quantification of Lupeol, Stigmasterol and β- Sitosterol in Extracts of Adhatoda vasica Nees Leaves and its Marketed Formulations by a Validated RP-HPLC Method up Drug of Action Cassia Alata Leaves Extract as Antiviral to Dengue Virus Serotype-2 in vitro ›