ArticleViewAbstractPharmacognosy Journal,202,12,6,1223-1231.DOI:10.5530/pj.2020.12.171Published:September 2020Type:Original ArticleIn vitro Evaluation of Acetylcholinesterase Inhibitory and Neuroprotective Activity in Commiphora species: A Comparative StudyPS Dhivya, P Selvamani, S Latha, Vasudevan Mani, and Nur Syamimi Mohd Azahan PS Dhivya1,*, P Selvamani2, S Latha3, Vasudevan Mani4, Nur Syamimi Mohd Azahan5 1Department of Pharmaceutical Technology, Anna University, Bharathidasan Institute of Technology Campus, Tiruchirappalli, Tamil Nadu, INDIA. 2Department of Pharmaceutical Technology, Centre for Excellence in Nanobio Translational Research (CENTRE), Anna University, Bharathidasan Institute of Technology Campus, Tiruchirappalli, Tamil Nadu, INDIA. 3Department of Pharmaceutical Technology, Centre for Excellence in Nanobio Translational Research (CENTRE), Anna University, Bharathidasan Institute of Technology Campus, Tiruchirappalli, Tamil Nadu, INDIA. 4Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraydah, KINGDOM OF SAUDI ARABIA. 5Faculty of Pharmacy, UniversitiTeknologi MARA (UiTM), 42300 Bandar PuncakAlam, Selangor, MALAYSIA. Abstract:Introduction: Herbal medicines are widely used in the therapeutic intervention that could delay the onset of Neurodegenerative diseases like, Alzheimer’s disease. Despite intensive advancements in the field of research currently available therapeutic strategies are often limited due to their adverse effects. Hence there is a need for the search for novel compounds for effective medications to treat neurocognitive deficits. Objective: The present study focused on acetylcholinesterase inhibitory and neuroprotective activity of Commiphora species plant for their possible use in Alzheimer’s disease. Methods: Phytochemical screening, TLC bioautographic and colourimetric assay was performed to quantify their acetylcholinesterase inhibitory activity of ethanolic extracts of the Commiphora species. The MTT assay was carried to evaluate the neuroprotective effect against the Aβ-induced cytotoxicity in SHSY5Y cell lines. Results: The bark of Commiphora berryi, leaves of Commiphora caudata and Commiphora pubescens were found abundant in flavonoids, glycosides, steroids and terpenoids. Further, the ethanol extract of Commiphora berryi (65.48% ± 0.10) showed the highest acetylcholinesterase inhibitory activity. The Aβ25-35 induced cell damage was as evidenced at a concentration of 20μM. The neuroprotective effect of the ethanol extracts was examined in neurodegenerative cells induced by Aβ25-35. On pretreatment with plant extracts, significant improvement in cell viability was observed as 63.42 ± 2.02% (25μg/ml) for Commiphora berryi. The maximum percentage of in-vitro AChE inhibition and neuroprotective effect was effective in the ethanol bark extract of Commiphora berryi. Conclusion: The results are very rousing to continue the screening of more unexplored plant species could be used for the development of novel bioactive compounds to treat the many diseases, especially for Alzheimer's disease. Keywords:Acetylcholinesterase, Aβ25-35, Commiphiora pubescens, Commiphora berryi, Commiphora caudata, Neuroprotective, SH-SY5Y Cell lineView:PDF (2.01 MB) PDF Images Phase contrast photomicrograph (10x) of SH-SY5Y cell viability by MTT assay ‹ Effect of Afghan Senjed (Elaeagnus Angustifolia L.) Leaves Aqueous alcoholic Extract on Blood Glucose Level of Diabetic Rats up In vitro Assay and Study Interaction of Uncaria gambir (Hunter) Roxb. as Anti-fibrotic Activity Against A549 Cell Line ›