ArticleViewAbstractPharmacognosy Journal,2021,13,4,965-970.DOI:10.5530/pj.2021.13.124Published:July 2021Type:Research ArticlePhytochemicals and Antioxidant Activities Evaluation of Origanum vulgare (L.) Stem Bark ExtractsRosmalena, Putu Ayu Widyastuti, Fatmawaty Yazid, Neneng Siti Silfi Ambarwati, and Islamudin Ahmad Rosmalena1, Putu Ayu Widyastuti2, Fatmawaty Yazid1, Neneng Siti Silfi Ambarwati3,*, Islamudin Ahmad4 1Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, South Jakarta, 10430 Jakarta, INDONESIA. 2Medical Student, Faculty of Medicine, Universitas Indonesia, South Jakarta, 10430 Jakarta, INDONESIA 3Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, East Jakarta, 13220 Jakarta, INDONESIA. 4Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, 75119 East Kalimantan, INDONESIA. Abstract:The present study aimed to evaluate phytochemical and antioxidant activity (in vitro and in vivo) of Origanum vulgare (L.) ethanolic extract. The phytochemical test was assessed using the Clule method in ethanol, ethyl acetate, and hexane. In vitro evaluation of antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) as an artificial free radical activity. In vivo test was conducted to evaluate the effect of malondialdehyde (MDA) level in blood plasma during maximum physical activity treatment. In vivo test was done using 25 male Sprague Dawley rats in pre and post-test control group design. The phytochemical test of O. vulgare ethanol extract was showed some compounds, such as a flavonoid, alkaloid, triterpenoid/steroid, essential oil, and tannin, then in ethyl acetate and hexane. In vitro assay showed that O. vulgare extract has strong antioxidant activity with an IC50 value of 133.47 μg/mL. While in the in vivo test, the most effective dosage is 20 mg/200 gr B.W., represented by a significant decrease of MDA level (0.509 nmol/mL) before and after treatment. So, the ethanolic extract of clove has potency as an herbal antioxidant because of the low level of IC50 and can decrease the MDA level. Keywords:2, 2-diphenyl-1-picrylhydrazyl, antioxidant activity, Malondialdehyde, Origanum vulgare (L.), PhytochemicalView:PDF (667.85 KB) PDF Images Graphical Abstract ‹ Repairing of Renal Tubules in Diabetic Rats (Rattus norvegicus) Diabetes After Administration of Golden Sea Cucumber (Stichopus hermanii) up Evaluation of Xanthine Oxidase Inhibitory and Antioxidant Activities of Three Organs of Idat (Cratoxylum glaucum Korth.) and Correlation with Phytochemical Cont ›