Evidence Based Evaluation of Pharmacological Activity and Herb-Herb interaction of Organic Extracts of Certain Natural Anti- Diabetic Mixtures

Objective: This work aims to find a new treatment based on the development of safe natural anti-diabetic mixtures. It assesses the hypoglycemic activity of natural mixtures and determines whether there are any negative side effects from the interaction of the herbs and the herbs. Methods: Six natural mixtures were tested for anti-diabetic activity, which was confirmed by a pathological histological examination. We performed a GC-MS analysis on active mixtures 1 and 2, yielding 54 and 38 compounds, respectively. The major compounds were Gingerol, Butan-2-one and 4-(3-hydroxy-2-methoxyphenyl) (97 and 64.02 per cent). Results: Among the six samples, the organic extract of mixture 1 and 2 showed a significant reduction in BGL compared to the standard drug glimepiride at a dose of 10 mg/kg ip and demonstrated a beneficial effect on renal function in alloxan-induced diabetic mice. These results were confirmed by a histopathological study which revealed that both mixture 1 and mixture 2 had decreased morphological and ultrastructural changes in the triggered liver. Docking of cuminaldehyde, Gingerol and α-Copaenin at the active site of human pancreatic enzymes α-amylase and aldol reductase revealed that these compounds had binding affinity at the active site of the enzymes. Conclusion: Our results revealed the anti-diabetic activity of non-polar mixtures consisting of long chain hydrocarbons, oils and non-polar components, thus suggesting that the herbal formulation is safe and effective for the treatment and complication of type 2 diabetes.