Protein Analysis of Royal Jelly Bee Apis Mellifera Carpatica as Candidates for Immunotherapy in Reproductive Disorders

Background: The honey bee of the Apis mellifera species is the main type of bee cultivated in almost all countries in the world, including Indonesia. Honey has benefits in various aspects, including food, health, and beauty. Royal jelly is a daily food menu as a food supplement to maintain and increase health and vitality. Purpose: Analyzing the immunogenic and toxinogenic proteins of Royal jelly Apis mellifera carpatica as immunotherapy. Method: Conversion of nucleotides into amino acids. Royal jelly Apis mellifera carpatica, analysis of the three-dimensional structure of Protein of Royal jelly Apis mellifera carpatica, Analysis of Ramachandran Plots of Royal jelly Apis mellifera carpatica, Analysis of epitope and allergen proteins, Analysis of antigens and toxins. Research result: The research results found that six proteins of Royal jelly Apis mellifera carpatica were epitope, antigenic, and non-toxic. Allergens and three Royal jelly proteins, Apis mellifera carpatica, are non-allergenic. In this study, the three-dimensional structure has not been found and opens opportunities for proteomic studies of the Royal jelly Apis mellifera carpatica protein, including protein isolation. Conclusion: The findings of this study can be used as a basis for the use of immunotherapy materials against the protein Royal jelly Apis mellifera carpatica against reproductive disorders.